Polymerase chain-reaction-mediated cloning and expression of the coat protein gene of potato virus Y inEscherichia coli

Virus Genes ◽  
1990 ◽  
Vol 4 (4) ◽  
pp. 339-350 ◽  
Author(s):  
Tatsuji Hataya ◽  
Teruo Sano ◽  
Kazusato Ohshima ◽  
Eishiro Shikata
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Potato Virus ◽  
Coat Protein Gene ◽  
Potato Virus Y ◽  
Protein Gene ◽  
Cloning And Expression ◽  
Inescherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Reverse-Transcription Polymerase Chain Reaction Detection of Citrus Tristeza Virus in Aphids

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1066-1069 ◽  
Author(s):  
Prem Mehta ◽  
R. H. Brlansky ◽  
S. Gowda ◽  
R. K. Yokomi
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Citrus Tristeza Virus ◽  
Protein Gene ◽  
Aphid Species ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tristeza Virus

A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology.

scholarly journals PENGGUNAAN PELACAK NONRADIOAKTIF (Digoxigenin-DNA Probe) UNTUK MENDETEKSI PEANUT STRIPE VIRUS

2001 ◽  
Vol 1 (2) ◽  
pp. 75-79
Author(s):  
Hasriadi Mat Akin
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Untranslated Region ◽  
Protein Gene ◽  
Dna Probe ◽  
Nuclear Inclusion ◽  
Chain Reaction ◽  
Peanut Stripe Virus ◽  
Polymerase Chain ◽  
Labeled Probe

The use of nonradioactive probe (Digoxigenin-DNA)  for  detection of peanut stripe virus.  The objective of this experiment was to develop the nonradioactive-labeled probe to detect peanut stripe virus (PStV) in peanut leaves and seeds. Digoxigenin labeled cDNA (dig-DNA probe) was synthesized from recombinant plasmid (pHS1.23) using polymerase chain reaction (PCR).  The probe containing 1.195 bp (base pair) corresponding to 3' termini, included part of NIb (nuclear inclusion body) gene, coat protein gene, and 3' untranslated region of PStV genome was used to detect the existence of PStV in peanut leaves and seeds of infected peanut plants.  

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