Use of the polymerase chain reaction to clone the potato leafroll virus coat protein gene directly from the total RNA of infected plants

1995 ◽  
Vol 38 (2) ◽  
pp. 211-218 ◽  
Author(s):  
G. Faccioli ◽  
A. Rosner ◽  
M. Forni
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Chain Reaction ◽  
Total Rna ◽  
Polymerase Chain ◽  
Virus Coat ◽  
Leafroll Virus

scholarly journals Nucleotide sequence of the potato leafroll virus coat protein gene

1989 ◽  
Vol 17 (4) ◽  
pp. 1768-1768 ◽  
Author(s):  
B. Prill ◽  
E. Maiss ◽  
U. Timpe ◽  
R. Casper
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Leafroll Virus

scholarly journals Reverse-Transcription Polymerase Chain Reaction Detection of Citrus Tristeza Virus in Aphids

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1066-1069 ◽  
Author(s):  
Prem Mehta ◽  
R. H. Brlansky ◽  
S. Gowda ◽  
R. K. Yokomi
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Citrus Tristeza Virus ◽  
Protein Gene ◽  
Aphid Species ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tristeza Virus

A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology.

scholarly journals PENGGUNAAN PELACAK NONRADIOAKTIF (Digoxigenin-DNA Probe) UNTUK MENDETEKSI PEANUT STRIPE VIRUS

2001 ◽  
Vol 1 (2) ◽  
pp. 75-79
Author(s):  
Hasriadi Mat Akin
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Untranslated Region ◽  
Protein Gene ◽  
Dna Probe ◽  
Nuclear Inclusion ◽  
Chain Reaction ◽  
Peanut Stripe Virus ◽  
Polymerase Chain ◽  
Labeled Probe

The use of nonradioactive probe (Digoxigenin-DNA)  for  detection of peanut stripe virus.  The objective of this experiment was to develop the nonradioactive-labeled probe to detect peanut stripe virus (PStV) in peanut leaves and seeds. Digoxigenin labeled cDNA (dig-DNA probe) was synthesized from recombinant plasmid (pHS1.23) using polymerase chain reaction (PCR).  The probe containing 1.195 bp (base pair) corresponding to 3' termini, included part of NIb (nuclear inclusion body) gene, coat protein gene, and 3' untranslated region of PStV genome was used to detect the existence of PStV in peanut leaves and seeds of infected peanut plants.  

scholarly journals Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids.

2005 ◽  
Vol 52 (3) ◽  
pp. 699-702 ◽  
Author(s):  
Elzbieta Sułuja ◽  
Ludmiła Strokowskaja ◽  
Włodzimierz Zagórski-Ostoja ◽  
Andrzej Pałucha
Keyword(s):  
Coat Protein ◽  
Nucleic Acids ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Recombinant Baculovirus ◽  
Potato Leafroll Virus ◽  
Rnase A ◽  
Virus Like Particles ◽  
Leafroll Virus

Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.

Molecular cloning and nucleotide sequence of the coat protein gene of a Cuban isolate of potato leafroll virus and its expression inEscherichia coli

Virus Genes ◽  
1994 ◽  
Vol 9 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Lisset López ◽  
Rita Muller ◽  
Ezequiel Balmori ◽  
Gustavo de la Riva ◽  
Nadia Ramírez ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Molecular Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Inescherichia Coli ◽  
Expression Inescherichia Coli ◽  
Leafroll Virus
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