The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population

1993 ◽  
Vol 13 (1) ◽  
pp. 29-39 ◽  
Author(s):  
J. Th�mmes ◽  
J. G�tgens ◽  
M. Biselli ◽  
P. W. Runstadler ◽  
C. Wandrey
2011 ◽  
Vol 236-238 ◽  
pp. 1005-1013
Author(s):  
Zhi Xi Hang ◽  
Qing Long Rao ◽  
Shi Yuan Yu

The influence of pH and dissolved oxygen tension (DOT) on mycelium growth and cellulase production by Trichoderma reesei was studied in this paper. The experiments were carried out with a cellulose of 10 g/l in a 10 L steam sterilizable bioreactor. The results have shown that H+ concentration was highly fluctuated in the growing and metabolizing periods of mycelium, which went against mycelium growth and cellulase production. Controlling pH to 4.8 was favorable to mycelium growth and cellulase production; the maximum mycelium mass concentration was increased from 2.60 g/l to 2.77 g/l; the maximum filter paper activity was raised from 1.87 IU/ml to 2.79 IU/ml. Meanwhile, the growth and metabolism of mycelium demand an appropriate dissolved oxygen tension (DOT). When the velocity of aeration was increased from 0.4 to 0.5vvm to improve the condition of dissolving oxygen, the mycelium mass concentration was increased from 2.77 g/l to 2.98g/l, and the filter paper activity was raised from 2.79 IU/ml to 2.98 IU/ml.


1991 ◽  
Vol 13 (4) ◽  
pp. 241-245 ◽  
Author(s):  
Loraine H. Anderson ◽  
Frank J. Roberts ◽  
Bernard Wilson ◽  
William J. Mehm

2005 ◽  
Vol 40 (2) ◽  
pp. 773-778 ◽  
Author(s):  
Gabriela Cortés ◽  
Mauricio A. Trujillo-Roldán ◽  
Octavio T. Ramı́rez ◽  
Enrique Galindo

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