Implantation of embryonic anlagen of the neocortex and spinal cord into an injured adult rat peripheral nerve

1990 ◽  
Vol 110 (2) ◽  
pp. 1113-1116
Author(s):  
E. I. Chumasov ◽  
E. S. Petrova
Keyword(s):  
Spinal Cord ◽  
Peripheral Nerve ◽  
Adult Rat ◽  
Rat Peripheral Nerve

scholarly journals Muscle Reinnervation with Delayed or Immediate Transplant of Embryonic Ventral Spinal Cord Cells into Adult Rat Peripheral Nerve

2002 ◽  
Vol 11 (3) ◽  
pp. 241-250 ◽  
Author(s):  
Robert M. Grumbles ◽  
Patrick Wood ◽  
Michelle Rudinsky ◽  
Anna M. Gomez ◽  
Christine K. Thomas
Keyword(s):  
Spinal Cord ◽  
Peripheral Nerve ◽  
Cell Transplantation ◽  
Muscle Atrophy ◽  
Skeletal Muscle Atrophy ◽  
Nerve Degeneration ◽  
Adult Rat ◽  
Muscle Reinnervation ◽  
The Mean ◽  
Ventral Spinal Cord

Muscle denervation is common in various neuromuscular diseases and after trauma. It induces skeletal muscle atrophy. Only muscle reinnervation leads to functional recovery. In previous studies, denervated adult rat muscles were rescued by transplantation of embryonic day 14–15 (E14–15) ventral spinal cord cells into a nearby peripheral nerve. In the present study, changes were made in the environment into which the cells were placed to test whether reinnervation was improved by: 1) prior nerve degeneration, induced by sciatic nerve transection 1 week before cell transplantation; 2) transplantation of 1 million versus 5 million cells; 3) addition of nerve growth factor (NGF) to the transplant. Ten weeks after cell transplantation, axons had grown from all of the transplants. The numbers of myelinated axons that regenerated into the tibial, medial (MG), and lateral gastrocnemius-soleus (LGS) nerves were similar across treatments. The mean diameters of large LGS axons (>6 μm) were significantly larger with nerve degeneration before transplantation. The mean diameters of MG and LGS axons were significantly larger with transplantation of 1 million versus 5 million cells. Silver-stained experimental and control lateral gastronemius (LG) muscles showed axons that terminated at motor end plates. Nodal and terminal sprouts were more common in reinnervated muscles (45–63% of all end plates) than in control muscles (10%). Electrical stimulation of the transplants induced weak contractions in 39 of 47 MG muscles (83%) and 33 of 46 LG muscles (72%) but at higher voltages than needed to excite control muscles. The threshold for MG contraction was lower with transplantation of 1 million cells, while LG thresholds were lower without NGF. The cross-sectional area of whole LG muscles was significantly larger with cell transplantation (immediate or delayed) than with media alone, but all of these muscle areas were reduced significantly compared with control muscle areas. These data suggest that delayed transplantation of fewer cells without NGF assists regeneration of larger diameter axons and prevents some muscle atrophy.

Co-transplantation of embryonic neural tissue and autologous peripheral nerve segments to severe spinal cord injury of the adult rat. Guided axogenesis from transplanted neurons

1991 ◽  
Vol 2 (4-6) ◽  
pp. 289-298
Author(s):  
Jean-Claude Horvat ◽  
Claude Baillet-Derbin ◽  
Jian Hui Ye ◽  
Fatiha Rhrich ◽  
Fatima Affane
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Peripheral Nerve ◽  
Neural Tissue ◽  
Adult Rat ◽  
Cord Injury

scholarly journals Neurofilament proteins of rat peripheral nerve and spinal cord.

1978 ◽  
Vol 78 (3) ◽  
pp. 653-662 ◽  
Author(s):  
W W Schlaepfer ◽  
L A Freeman
Keyword(s):  
Spinal Cord ◽  
Peripheral Nerve ◽  
Osmotic Shock ◽  
Sodium Dodecyl ◽  
Minor Component ◽  
Tissue Homogenates ◽  
Central Nervous Systems ◽  
Rat Peripheral Nerve ◽  
Staining Techniques ◽  
A Minor

Intact neurofilaments were isolated in parallel from rat peripheral nerve and spinal cord by osmotic shock into hypotonic media containing divalent cation chelators. Isolated neurofilaments were washed and separated by multiple centrifugations in 0.1 M NaCl. Abundant intact neurofilaments were identified in the washed pellets by negative staining techniques. Their origin from neurofilaments was confirmed by immune electron microscopy. Washed neurofilaments were extracted from lipid and membranous components with 8 M urea. Analyses of neurofilament isolates on sodium dodecyl sulfate gels showed that proteins of 200,000, 150,000, and 69,000 mol wt were the major components of intact neurofilaments derived from rat peripheral and central nervous systems. These same proteins were identified in whole tissue homogenates of both sources and became enriched during the isolation of intact neurofilaments. A minor component of 64,000 mol wt arose during isolation. Other proteins were identified as contaminants. Small amounts of proteins with electrophoretic migration of tubulin and actin remain in neurofilament isolates.

Cryo-immunogold ultrastructural localization of laminin in adult rat peripheral nerve

1995 ◽  
Vol 24 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Gerald J. Little ◽  
C. Stephen Robinson ◽  
John W. Heath
Keyword(s):  
Peripheral Nerve ◽  
Ultrastructural Localization ◽  
Adult Rat ◽  
Rat Peripheral Nerve

Long-term delivery of FGF-6 changes the fiber type and fatigability of muscle reinnervated from embryonic neurons transplanted into adult rat peripheral nerve

2007 ◽  
Vol 85 (9) ◽  
pp. 1933-1942 ◽  
Author(s):  
Robert M. Grumbles ◽  
Gizelda T. B. Casella ◽  
Michelle J. Rudinsky ◽  
Patrick M. Wood ◽  
Sanjay Sesodia ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Fiber Type ◽  
Adult Rat ◽  
Term Delivery ◽  
Rat Peripheral Nerve ◽  
Embryonic Neurons
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