An enzyme-linked immunosorbent assay for the determination of chloramphenicol using a monoclonal antibody Application to residues in swine muscle tissue

AbstractHomoharringtonine (HHT), also known as omacetaxine, is a natural compound found in the genus Cephalotaxus and is a promising pharmaceutical drug used for the treatment of chronic or accelerated phase chronic myeloid leukemia. As a tool for the quantitative determination of HHT, a specific monoclonal antibody against HHT (MAb 6A1) was generated by conjugates prepared via sodium periodate-mediated oxidation. The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be highly specific and sensitive with a limit of detection for HHT of 48.8 ng/mL. Validation assays to evaluate precision and accuracy of the method were conducted by the use of intra- and inter-assay analysis, recovery test, and comparison analysis between the amounts of HHT determined by ELISA and high-performance liquid chromatography. These results revealed that the established icELISA using MAb 6A1 is specific, sensitive, and reliable enough to be applied to the qualitative analysis for HHT. Furthermore, the results of this study support the usefulness of sodium periodate as a reagent for the conjugation between Cephalotaxus alkaloids and proteins for producing specific antibodies.

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Development of a highly sensitive and specific monoclonal antibody-based enzyme-linked immunosorbent assay for determination of doxycycline in chicken muscle, liver and egg

Food Chemistry ◽

10.1016/j.foodchem.2012.04.030 ◽

2012 ◽

Vol 134 (4) ◽

pp. 2442-2446 ◽

Cited By ~ 15

Author(s):

Tao Le ◽

Zhengwu Zhao ◽

Wei Wei ◽

Dingren Bi

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Monoclonal Antibody ◽

Chicken Muscle ◽

Highly Sensitive

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A Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Determination of Olaquindox in Animal Feed

Analytical Letters ◽

10.1080/00032719.2013.862627 ◽

2014 ◽

Vol 47 (6) ◽

pp. 1015-1030 ◽

Cited By ~ 6

Author(s):

Lei Wang ◽

Jingyan Zhang ◽

Dongan Cui ◽

Xuezhi Wang ◽

Zhiqiang Yang ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Animal Feed ◽

Enzyme Linked Immunosorbent Assay

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Enzyme-linked immunosorbent assay for chromogranin A.

Clinical Chemistry ◽

10.1093/clinchem/35.9.1934 ◽

1989 ◽

Vol 35 (9) ◽

pp. 1934-1938 ◽

Cited By ~ 27

Author(s):

L Dillen ◽

J De Block ◽

L Van Lear ◽

W De Potter

Keyword(s):

Monoclonal Antibody ◽

Human Plasma ◽

Immunosorbent Assay ◽

Coefficient Of Variation ◽

Chromogranin A ◽

Enzyme Linked Immunosorbent Assay ◽

Chromaffin Granules ◽

Standard Curve

Abstract This is an enzyme-linked immunosorbent assay (ELISA) for determining chromogranin A (CGA) with use of a monoclonal antibody. CGA was isolated from bovine chromaffin granules. The analytical ELISA procedure for bovine CGA was developed and optimized. Typical standard curves ranged from 500 pg to 500 ng of CGA. We then studied human plasma CGA-immunoreactivity as measured by this assay. The curve for dilutions of human plasma paralleled the standard curve for bovine CGA. The intra-assay coefficient of variation for determination of human plasma CGA was 4.56%, indicating that reliable determinations can be performed for human plasma. However, further study revealed the presence of two CGA-immunoreactive substances in human plasma, one of which corresponds to the native CGA. The nature of the second immunoreactive substance still remains unknown. Nevertheless the measured CGA concentrations (ranging from 0.19 to 0.35 mg/L) in plasma are comparable with previously reported values.

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Determination of quinoxaline antibiotics in fish feed by enzyme-linked immunosorbent assay using a monoclonal antibody

Analytical Methods ◽

10.1039/c5ay00953g ◽

2015 ◽

Vol 7 (12) ◽

pp. 5204-5209 ◽

Cited By ~ 23

Author(s):

Juan Peng ◽

Dezhao Kong ◽

Liqiang Liu ◽

Shanshan Song ◽

Hua Kuang ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Fish Feed

Olaquindox (OLA), mequindox (MEQ), and quincetone (QCT) are widely used synthetic antibiotics of the quinoxaline-1,4-dioxide family.

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Development of a sensitive monoclonal antibody-based ELISA for the determination of a β-adrenergic agonist brombuterol in swine meat, liver and feed samples

Analytical Methods ◽

10.1039/c6ay01709f ◽

2016 ◽

Vol 8 (38) ◽

pp. 6941-6948 ◽

Cited By ~ 8

Author(s):

Yingying Zhao ◽

Danni Jiang ◽

Kang Wu ◽

Hong Yang ◽

Haijing Du ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Adrenergic Agonist ◽

Feed Samples

A sensitive enzyme-linked immunosorbent assay (ELISA) based on a newly prepared monoclonal antibody (mAb) for the determination of a β-adrenergic agonist brombuterol in swine meat, liver and feed samples was developed.

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Simultaneous Determination of Florfenicol and Its Metabolite Florfenicol Amine in Swine Muscle Tissue by a Heterologous Enzyme-Linked Immunosorbent Assay

Journal of AOAC International ◽

10.1093/jaoac/92.3.981 ◽

2009 ◽

Vol 92 (3) ◽

pp. 981-988 ◽

Cited By ~ 13

Author(s):

Pengjie Luo ◽

Haiyang Jiang ◽

Zhanhui Wang ◽

Caimao Feng ◽

Fangyang He ◽

...

Keyword(s):

Simultaneous Determination ◽

Muscle Tissue ◽

Immunosorbent Assay ◽

Simultaneous Detection ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

Coefficients Of Variation ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic

Abstract A sensitive and heterologous enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of florfenicol (FF) and its metabolite florfenicol amine (FFA) in swine muscle tissue was developed. FFA was conjugated to bovine serum albumin by a formaldehyde coupling method as an immunogen to immunize rabbits. FFA, thiamphenicol glycinate, and modified FF were conjugated to ovalbumin as coating antigens. The effect of different types of hapten heterology on the sensitivity and specificity of the ELISA was evaluated. Using FF glutaric anhydride ester as a coating hapten and antibody raised against modified FFA, an ELISA was developed that showed an IC50 value of 0.48 ng/mL. The antibody showed a cross-reactivity of 100 with FFA, 97 with FF, 6 with thiamphenicol, and a negligible value with chloramphenicol. From fortified swine muscle samples at levels of 4320 ng/g, the average recoveries of FF and FFA ranged from 58.2 to 96.8, with coefficients of variation less than 14. Analysis of incurred samples by the ELISA gave similar results to those by a previously developed liquid chromatographic method. The ELISA could be used as a rapid method for the simultaneous determination of FF and FFA in swine muscle tissue.

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