Development of a highly sensitive and specific monoclonal antibody-based enzyme-linked immunosorbent assay for determination of doxycycline in chicken muscle, liver and egg

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

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Determination of Urinary Acetylpolyamines by a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay (ELISA)

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a125009 ◽

1995 ◽

Vol 118 (6) ◽

pp. 1211-1215 ◽

Cited By ~ 6

Author(s):

Kunio Fujiwara ◽

Hiroshi Kanetake ◽

Koichi Furukawa ◽

Yukinobu Masuyama ◽

Gang Bai ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay

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Comparison of a monoclonal antibody-based enzyme-linked immunosorbent assay and gas chromatography for the determination of nicotine in cigarette smoke condensates

Analytical Chemistry ◽

10.1021/ac00070a010 ◽

1993 ◽

Vol 65 (22) ◽

pp. 3227-3231 ◽

Cited By ~ 4

Author(s):

Antonio. Abad ◽

Juan J. Manclus ◽

Carmen. March ◽

Angel. Montoya

Keyword(s):

Monoclonal Antibody ◽

Gas Chromatography ◽

Cigarette Smoke ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay

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A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Determination of Homoharringtonine

Planta Medica ◽

10.1055/a-0578-8689 ◽

2018 ◽

Vol 84 (14) ◽

pp. 1038-1044 ◽

Cited By ~ 3

Author(s):

Benyakan Pongkitwitoon ◽

Seiichi Sakamoto ◽

Rika Nagamitsu ◽

Waraporn Putalun ◽

Hiroyuki Tanaka ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Myeloid Leukemia ◽

High Performance ◽

Sodium Periodate ◽

Limit Of Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Study Support ◽

Mediated Oxidation

AbstractHomoharringtonine (HHT), also known as omacetaxine, is a natural compound found in the genus Cephalotaxus and is a promising pharmaceutical drug used for the treatment of chronic or accelerated phase chronic myeloid leukemia. As a tool for the quantitative determination of HHT, a specific monoclonal antibody against HHT (MAb 6A1) was generated by conjugates prepared via sodium periodate-mediated oxidation. The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be highly specific and sensitive with a limit of detection for HHT of 48.8 ng/mL. Validation assays to evaluate precision and accuracy of the method were conducted by the use of intra- and inter-assay analysis, recovery test, and comparison analysis between the amounts of HHT determined by ELISA and high-performance liquid chromatography. These results revealed that the established icELISA using MAb 6A1 is specific, sensitive, and reliable enough to be applied to the qualitative analysis for HHT. Furthermore, the results of this study support the usefulness of sodium periodate as a reagent for the conjugation between Cephalotaxus alkaloids and proteins for producing specific antibodies.

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