Characterization of the somatostatin-like immunoreactivity extracted from an adrenal medullary tumour

1982 ◽  
Vol 2 (3) ◽  
pp. 147-154 ◽  
Author(s):  
R. Corder ◽  
J. E. C. Sykes ◽  
P. J. Lowry
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Gel Filtration ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Identical Composition ◽  
Salt Fractionation ◽  
Somatostatin 14

Significant amounts of somatostatin-like immunor reactivity (SLI) were detected in the extract of a human catecholamine-secreting adrenal medullary tumour. After salt fractionation and reconstitution the major portion of SLI was purified by gel filtration and two HPLC steps; in all three systems it eluted in the position of somatostatin-14. The purified somatostatin-like peptide inhibited, in a dose-related manner, growth hormone release from stimulated perfused rat anterior pituitary ceils in vitro. Amino acid analysis showed the purified peptide to have an identical composition to somatostatin found in other species.

GRF increases release of growth hormone and arachidonate from anterior pituitary cells

1985 ◽  
Vol 248 (4) ◽  
pp. E438-E442
Author(s):  
A. M. Judd ◽  
K. Koike ◽  
R. M. MacLeod
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Dependent Manner ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Concentration Dependent Manner ◽  
Anterior Pituitary Cells ◽  
Arachidonate Release

Arachidonate and its metabolites increase growth hormone release in vitro. A study was designed to determine whether arachidonate release from anterior pituitary cells is modified by growth hormone-releasing factor (GRF) or somatostatin (SRIF). Cultured pituitary cells were incubated with [3H]arachidonate to esterify the long-chain fatty acid into cellular lipids. The cells were extensively washed with medium containing no [3H]arachidonate and then incubated with GRF and/or SRIF for 30 min. The incubation medium was then extracted with ethyl acetate, and following thin-layer chromatographic separation, the radioactivity in the [3H]arachidonate band was measured. GRF in a concentration-dependent manner (1-30 nM) stimulated growth hormone and arachidonate release, whereas SRIF (100 nM) blocked the GRF-induced increase of growth hormone and arachidonate release. The effects of GRF on growth hormone and arachidonate were evident at time intervals as brief as 5 min. These findings support the hypothesis that arachidonate may play a role in the GRF-induced growth hormone release.

Characterization of the stimulatory effect of galanin on growth hormone release from the rat anterior pituitary

Life Sciences ◽  
1991 ◽  
Vol 48 (17) ◽  
pp. 1639-1644 ◽  
Author(s):  
Makoto Sato ◽  
Jiro Takahara ◽  
Michio Niimi ◽  
Reiko Tagawa ◽  
Shozo Irino
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Hormone Release ◽  
Growth Hormone Release

Demonstration of biological activity of a growth hormone-releasing hormone-like substance produced by a pheochromocytoma

1993 ◽  
Vol 129 (3) ◽  
pp. 246-250 ◽  
Author(s):  
Haruhiko Saito ◽  
Toshiaki Sano ◽  
Ryuichi Yamasaki ◽  
Shinji Mitsuhashi ◽  
Eiji Hosoi ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Biological Activity ◽  
Gel Filtration ◽  
Molecular Heterogeneity ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone

The biological characteristics of a growth hormone-releasing hormone (GHRH)-like substance produced by a pheochromocytoma were studied. Analysis by gel filtration chromatography combined with the use of two distinct GHRH antisera that recognize the N- and C-termini of authentic GHRH(1–44)NH2 indicated molecular heterogeneity of the immunoreactive GHRH in the tumor extract, but a component corresponding to GHRH(1–44)NH2 was the predominant form. The biological activity of this immunoreactive component was assessed in vitro by measuring its ability to induce growth hormone release from dispersed rat anterior pituitary cells. At concentrations of 0.125–2.0 nmol/l, the test materials induced a dose-related increase in growth hormone release from the cells into the incubation medium (range 992±68–1872±32 ng·1.7×105 cells−1·3 h−1), similar to that observed with synthetic GHRH(1–44)NH2 (control value 640±30 ng·1.7× 105 cells−1·3 h−1). This suggests that immunoreactive GHRH in the tumor has almost the same biological activity as the synthetic product and that a combination of pheochromocytoma and acromegaly is not always fortuitous because both diseases may be caused by a single neoplasm.

Differential Effects of Ketoconazole on Prolactin and Growth Hormone Release by Normal and Tumoral Rat Anterior Pituitary Cells in vitro

1989 ◽  
Vol 50 (5) ◽  
pp. 513-518 ◽  
Author(s):  
Robert Drouhault ◽  
Pierre Vacher ◽  
Jean P. David ◽  
Anne M. Courtes ◽  
Nidaravone Vilayleck ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Differential Effects ◽  
Anterior Pituitary Cells

Biphasic Effects of Dexamethsone on Growth Hormone Release in vitro.

1987 ◽  
Vol 34 (6) ◽  
pp. 947-953 ◽  
Author(s):  
KOJI NAKAGAWA ◽  
KAZUMASA AKIKAWA ◽  
MIYAO MATSUBARA ◽  
MITSUMASA KUBO ◽  
TATSUYA ISHIZUKA ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Release In Vitro
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