Analysis by partitioning in aqueous two-phase systems of the loss of transferrin-binding capacity during maturation of rat reticulocytes

1989 ◽  
Vol 9 (5) ◽  
pp. 541-548 ◽  
Author(s):  
J. Mendieta ◽  
A. Herráez ◽  
P. Sancho ◽  
J. Luque
Keyword(s):  
Binding Capacity ◽  
Cell Populations ◽  
Red Cell ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Number Of Binding Sites ◽  
Reticulocyte Maturation ◽  
Phase Systems ◽  
The Relationship ◽  
Rat Reticulocytes

A decrease in the number of binding sites for125I-transferrin, without an apparent modification of the association constant, has been observed during the maturation of reticulocytes into erythrocytes. As an experimental model, different red cell populations from phenylhydrazinic anaemic rates (95% to 12% reticulocyte-rich) have been used. The fractionation by multiple partition in two-phase systems of these red cell populations has been applied here to show the relationship between number of transferrin receptors and rate of reticulocyte maturation.

Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes

1987 ◽  
Vol 7 (2) ◽  
pp. 113-119 ◽  
Author(s):  
J. Luque ◽  
M. D. Delgado ◽  
P. Rodriguez-Horche ◽  
M. T. Company ◽  
M. Pinilla
Keyword(s):  
Ethylene Glycol ◽  
Pyruvate Kinase ◽  
Significant Variation ◽  
Cell Populations ◽  
Red Cell ◽  
Two Phase ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Bisphosphoglycerate Mutase

An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextrahpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity.

Surface heterogeneity of rat sperm during maturation

1992 ◽  
Vol 12 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Elizabeth J. Cartwright ◽  
Pauline Harrington ◽  
Louise Norbury ◽  
Gareth Leeming ◽  
Paul T. Sharpe
Keyword(s):  
Surface Properties ◽  
Vas Deferens ◽  
Surface Heterogeneity ◽  
Cell Populations ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partition Surface ◽  
Sperm Population

Rat sperm isolated from the caput and caudal epididymis and the vas deferens were subjected to multiple partition in aqueous two-phase systems. The technique was used to reveal heterogeneity of a sperm population with respect to particular surface properties. Sperm from all three regions gave broad distributions indicative of heterogeneous cell populations. Greatest heterogeneity was observed for cauda sperm with caput and was sperm producing similar distributions. Following multiple partition sperm from different regions of the distribution profiles were immunostained with three antibodies known to recognise maturation antigens. The results show that some antigens are acquired during epididymal transit whilst others are present throughout. The partition (surface heterogeneity) seen cannot therefore be explained solely by the distribution of the antigens recognised by 2D6, 6B2 and 3D5.

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

2021 ◽  
Vol 60 (15) ◽  
pp. 5403-5410
Author(s):  
Martha Alicia González-Félix ◽  
Luis Alberto Mejía-Manzano ◽  
Bertha A. Barba-Dávila ◽  
Sergio O. Serna-Saldívar ◽  
José González-Valdez
Keyword(s):  
Two Phase ◽  
Green Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Recovery of value-added products from wastewater using Aqueous Two-Phase Systems – A review

2021 ◽  
Vol 778 ◽  
pp. 146293
Author(s):  
Thivaharan Varadavenkatesan ◽  
Shraddha Pai ◽  
Ramesh Vinayagam ◽  
Arivalagan Pugazhendhi ◽  
Raja Selvaraj
Keyword(s):  
Value Added ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Value Added Products

Fabrication of Microgel Particles with Complex Shape via Selective Polymerization of Aqueous Two-Phase Systems

Small ◽  
2012 ◽  
Vol 8 (15) ◽  
pp. 2356-2360 ◽  
Author(s):  
Shaohua Ma ◽  
Julian Thiele ◽  
Xin Liu ◽  
Yunpeng Bai ◽  
Chris Abell ◽  
...  
Keyword(s):  
Complex Shape ◽  
Two Phase ◽  
Microgel Particles ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Characterization of Aqueous Two-Phase Systems from Volume and Density Measurements

2016 ◽  
Vol 61 (4) ◽  
pp. 1531-1539 ◽  
Author(s):  
Ehsan Atefi ◽  
Darcy Fyffe ◽  
Kerim B. Kaylan ◽  
Hossein Tavana
Keyword(s):  
Two Phase ◽  
Density Measurements ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Phase Behavior in Aqueous Two-Phase Systems Containing Micelle-Forming Block Copolymers

1995 ◽  
Vol 28 (10) ◽  
pp. 3597-3603 ◽  
Author(s):  
Maarten Svensson ◽  
Per Linse ◽  
Folke Tjerneld
Keyword(s):  
Block Copolymers ◽  
Phase Behavior ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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