Surface heterogeneity of rat sperm during maturation

1992 ◽  
Vol 12 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Elizabeth J. Cartwright ◽  
Pauline Harrington ◽  
Louise Norbury ◽  
Gareth Leeming ◽  
Paul T. Sharpe
Keyword(s):  
Surface Properties ◽  
Vas Deferens ◽  
Surface Heterogeneity ◽  
Cell Populations ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partition Surface ◽  
Sperm Population

Rat sperm isolated from the caput and caudal epididymis and the vas deferens were subjected to multiple partition in aqueous two-phase systems. The technique was used to reveal heterogeneity of a sperm population with respect to particular surface properties. Sperm from all three regions gave broad distributions indicative of heterogeneous cell populations. Greatest heterogeneity was observed for cauda sperm with caput and was sperm producing similar distributions. Following multiple partition sperm from different regions of the distribution profiles were immunostained with three antibodies known to recognise maturation antigens. The results show that some antigens are acquired during epididymal transit whilst others are present throughout. The partition (surface heterogeneity) seen cannot therefore be explained solely by the distribution of the antigens recognised by 2D6, 6B2 and 3D5.

Surface heterogeneity of bovine sperm revealed by aqueous two-phase partition

1991 ◽  
Vol 11 (5) ◽  
pp. 265-273 ◽  
Author(s):  
Elizabeth J. Cartwright ◽  
Allison Cowin ◽  
Paul T. Sharpe
Keyword(s):  
Surface Properties ◽  
Seminal Fluid ◽  
Surface Heterogeneity ◽  
Two Phase ◽  
Surface Molecules ◽  
Affinity Ligand ◽  
Bovine Sperm ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Two Phase Partition

Ejaculated, bovine sperm have been subjected to multiple partition in aqueous two-phase systems. This partition, carried out in a countercurrent fashion, reveals heterogeneity of the sperm population with respect to surface properties. The sperm, when partitioned in phase systems that detect non-change associated surface properties (change-insensitive) are largely distributed as two distinct populations. In charge-sensitive phase systems (which principally detect cell surface molecules carrying charge) the sperm do not show any obvious surface heterogeneity. Considerable heterogeneity is revealed in affinity-ligand phase systems containing palmitic acid coupled to one of the phase components-poly(ethylene glycol). There is a difference in surface heterogeneity between sperm which have been washed in buffer or left unwashed, direct from the ejaculate. This is indicative of weak adsorption of proteins to the sperm surface in seminal fluid. These results show that bovine ejaculated sperm is a heterogeneous cell population having unequal distributions of a number of different surface molecules.

Analysis by partitioning in aqueous two-phase systems of the loss of transferrin-binding capacity during maturation of rat reticulocytes

1989 ◽  
Vol 9 (5) ◽  
pp. 541-548 ◽  
Author(s):  
J. Mendieta ◽  
A. Herráez ◽  
P. Sancho ◽  
J. Luque
Keyword(s):  
Binding Capacity ◽  
Cell Populations ◽  
Red Cell ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Number Of Binding Sites ◽  
Reticulocyte Maturation ◽  
Phase Systems ◽  
The Relationship ◽  
Rat Reticulocytes

A decrease in the number of binding sites for125I-transferrin, without an apparent modification of the association constant, has been observed during the maturation of reticulocytes into erythrocytes. As an experimental model, different red cell populations from phenylhydrazinic anaemic rates (95% to 12% reticulocyte-rich) have been used. The fractionation by multiple partition in two-phase systems of these red cell populations has been applied here to show the relationship between number of transferrin receptors and rate of reticulocyte maturation.

scholarly journals Characterization of Surface Properties of Heat Shock Proteins for the Separation Using Aqueous Two-Phase Systems.

1994 ◽  
Vol 27 (6) ◽  
pp. 808-814 ◽  
Author(s):  
Koji Yano ◽  
Tetsuhiro Hasegawa ◽  
Ryoichi Kuboi ◽  
Isao Komasawa ◽  
Tetsuaki Tsuchido
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Surface Properties ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Shock Proteins

scholarly journals Differentiation of surface properties of chlorococcalean algae by means of aqueous two phase systems

2014 ◽  
Vol 58 (1) ◽  
pp. 59-64
Author(s):  
Jan Burczyk ◽  
Dorota Szymakowska
Keyword(s):  
Surface Properties ◽  
Two Phase ◽  
Ph Values ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Algal cells belonging to various strains of <em>Chlorococcales (Chlorophyta) </em>have been partitioned in aqueous two-phase systems containing ionogenic polymers, DEAE-dextran or SDS-dextran, at various pH values. Strain-specific differences of partition type which have been found in the phase systems used can be useful for distinguishing of algal cells.

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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