Importance of actin cytoskeleton behaviour during preservation of carrot cell suspensions in liquid nitrogen

PROTOPLASMA ◽  
1993 ◽  
Vol 173 (1-2) ◽  
pp. 35-47 ◽  
Author(s):  
Christiane Morisset ◽  
C. Gazeau ◽  
J. Hansz ◽  
J. Dereuddre
Keyword(s):  
Actin Cytoskeleton ◽  
Liquid Nitrogen ◽  
Cell Suspensions ◽  
Carrot Cell

Xylose transfer to lipids by membranes of carrot cell suspensions

1993 ◽  
Vol 33 (6) ◽  
pp. 1347-1352 ◽  
Author(s):  
Luis A. Quesada-Allué ◽  
Karin Hagelin ◽  
Fiorella LoSchiavo
Keyword(s):  
Cell Suspensions ◽  
Carrot Cell

scholarly journals Effects of preculture with sucrose and aba on cell suspensions water status and its relation with vitrification resistance

2000 ◽  
Vol 12 (2) ◽  
pp. 166-180 ◽  
Author(s):  
GUILLERMO SEIJO
Keyword(s):  
Cell Viability ◽  
Cell Survival ◽  
Water Potential ◽  
Liquid Nitrogen ◽  
Osmotic Shock ◽  
Water Status ◽  
Cell Suspensions ◽  
Cell Water ◽  
Solute Accumulation ◽  
Low Water Potential

Changes in cell water relations during precultures were followed in an attempt to understand the mechanism of cell hardening for cryopreservation by vitrification. Medium containing 0.4 M sucrose (psiw=-1.45 MPa) and containing 5 mg L-1 of ABA (MT psiw=-0.73 MPa and MS psiw=-0.48 MPa) were used to harden cell suspensions of orange and carrot. Preculture in these medium did not cause a significant decrease of cell viability, however, it improved the cell survival to PVS2 and liquid nitrogen expositions. When cells were inoculated into medium containing 0.4 M sucrose, the cell psiw decreased rapidly until reach the medium psiw , and turgor was also severely reduced or disappeared. Subsequently, cell psis began to decrease and after 48 h of treatment in low water potential medium, turgor was recovered by osmoregulation to almost the same values that cells had at the beginning of the experiment. Preculture in medium containing ABA also caused a decrease of cell psis but in much less extent than the preculture in low water potential medium. Since both precultures improved significantly the survival to PVS2 pretreatment and to LN exposure, this increment of survival to osmotic shock and vitrification can not be attribute solely to quantitative solute accumulation. The results suggest that qualitative accumulation of determined solutes would be the events which contributed for the improvement of tolerance to PVS2 exposure and thence tolerance to liquid nitrogen.

Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions

2007 ◽  
Vol 44 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Natalia Loukanina ◽  
Trevor A. Thorpe
Keyword(s):  
Cell Suspensions ◽  
Carrot Cell

scholarly journals Effect of Enzymes on Serum and Particle Properties of Carrot Cell Suspensions

2015 ◽  
Vol 10 (4) ◽  
pp. 428-438
Author(s):  
Ashwin K Sankaran ◽  
Jaap Nijsse ◽  
Ruth Cardinaels ◽  
Lucy Bialek ◽  
Avi Shpigelman ◽  
...  
Keyword(s):  
Cell Suspensions ◽  
Particle Properties ◽  
Carrot Cell

Embryogenesis and ribosomal DNA in carrot cell suspensions cultured in vitro

1984 ◽  
Vol 33 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Kiyoshi Masuda ◽  
Yoshio Kikuta ◽  
Yozo Okazawa
Keyword(s):  
Ribosomal Dna ◽  
Cell Suspensions ◽  
Carrot Cell

Gene expression during induction of somatic embryogenesis in carrot cell suspensions

Planta ◽  
1991 ◽  
Vol 183 (1) ◽  
Author(s):  
F. Aleith ◽  
G. Richter
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Cell Suspensions ◽  
Carrot Cell

Utilization of irradiated carrot cell suspensions as feeder layer for cultured Nicotiana cells and protoplasts

1980 ◽  
Vol 19 (3) ◽  
pp. 243-252 ◽  
Author(s):  
Rino Cella ◽  
Esra Galun
Keyword(s):  
Cell Suspensions ◽  
Feeder Layer ◽  
Carrot Cell

scholarly journals Ammonium Assimilation and the Role of [gamma]-Aminobutyric Acid in pH Homeostasis in Carrot Cell Suspensions

1994 ◽  
Vol 106 (2) ◽  
pp. 513-520 ◽  
Author(s):  
A. D. Carroll ◽  
G. G. Fox ◽  
S. Laurie ◽  
R. Phillips ◽  
R. G. Ratcliffe ◽  
...  
Keyword(s):  
Ammonium Assimilation ◽  
Cell Suspensions ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Ph Homeostasis ◽  
Carrot Cell

Rapid isolation and purification of plastid and mitochondrial DNA from carrot cell suspensions

1984 ◽  
Vol 2 (3) ◽  
pp. 32-36 ◽  
Author(s):  
Lorin R. DeBonte ◽  
Benjamin F. Matthews
Keyword(s):  
Mitochondrial Dna ◽  
Cell Suspensions ◽  
Isolation And Purification ◽  
Rapid Isolation ◽  
Carrot Cell
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