D-14 Monoclonal antibody to carcinoembryonic antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded human colorectal carcinoma, tumors of non-colorectal origin and normal tissues

1990 ◽  
Vol 116 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Zlatko P. Pavelic ◽  
Nicholas J. Petrelli ◽  
Lemuel Herrera ◽  
Mary M. Vaughan ◽  
Jeffrey S. Paecock ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Colorectal Carcinoma ◽  
Carcinoembryonic Antigen ◽  
Immunohistochemical Analysis ◽  
Normal Tissues ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Colorectal Origin ◽  
Human Colorectal Carcinoma ◽  
Formalin Fixed

Antigen Expression in Normal and Neoplastic Canine Tissues Defined by a Monoclonal Antibody Generated against Canine Mesothelioma Cells

1994 ◽  
Vol 31 (6) ◽  
pp. 663-673 ◽  
Author(s):  
K. X. Liu ◽  
A. E. Church Bird ◽  
S. D. Lenz ◽  
S. P. McDonough ◽  
L. G. Wolfe
Keyword(s):  
Monoclonal Antibody ◽  
Smooth Muscle ◽  
Soft Tissues ◽  
Mesothelial Cell ◽  
Bladder Smooth Muscle ◽  
Normal Tissues ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Abc Method ◽  
Formalin Fixed

Monoclonal antibody (MAb) 3B5 generated against canine mesothelioma cells was applied to canine tumors and normal tissues via immunohistochemical and immunoblotting techniques to evaluate antigen binding. By use of an avidin-biotin immunoperoxidase complex (ABC) method, immunoreactivity was noted in reactive mesothelial cells and in normal tissues was observed primarily in mesothelial cell linings, endothelial cells, and smooth muscle of blood vessels and soft tissues; the reactivity was nearly equivalent in frozen or formalin-fixed, paraffin-embedded tissue sections. Use of the ABC method on formalin-fixed, paraffin-embedded tumors yielded moderate to strong cytoplasmic immunostaining of neoplastic cells in 10/11 (91%) mesotheliomas, 18/23 (78%) hemangiosarcomas, 4/10 (40%) intestinal and lung carcinomas, and ≤20% of hemangiomas, leiomyosarcomas, leiomyomas, mammary carcinomas, and squamous cell carcinomas. No immunostaining of tumor cells was observed in fibrosarcomas, hemangiopericytomas, perianal gland carcinomas, and melanomas. Immunoblotting was performed on samples that demonstrated strong immunoreactivity with MAb 3B5 by the ABC method: mesothelioma, hemangiosarcoma, urinary bladder (smooth muscle), and lung (alveolar capillaries). These analyses showed that MAb 3B5 bound a major antigen of 78 kilodaltons (kd) and minor antigens at 56 and 54 kd in normal and neoplastic tissues. The preliminary immunohistochemical results suggest that MAb 3B5 may possess utility in diagnosis of mesotheliomas and hemangiosarcomas, discrimination of cell types in proliferative serosal lesions, and demonstration of vascularity or angiogenesis in neoplastic and inflammatory lesions.

scholarly journals Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue.

1982 ◽  
Vol 30 (4) ◽  
pp. 371-377 ◽  
Author(s):  
R D McComb ◽  
T R Jones ◽  
S V Pizzo ◽  
D D Bigner
Keyword(s):  
Endothelial Cells ◽  
Von Willebrand Factor ◽  
Immunohistochemical Detection ◽  
Normal Tissues ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Factor Antigen ◽  
Formalin Fixed

The immunohistochemical detection of factor VIII/von Willebrand factor antigen (FVIII/vWF-AG) in formalin-fixed paraffin-embedded tissues was investigated using the peroxidase-antiperoxidase (PAP) method. Highly purified human FVIII/vWF was used to raise rabbit anti-FVIII/vWF-AG serum. In addition to anti-FVIII/vWF-AG activity, the unabsorbed antiserum had anti-IgG, anti-IgM, and anti-alpha2-macroglobulin specificities. Following exhaustive absorption with these proteins, the antiserum reacted monospecifically for FVIII/vWF-AG in immunodiffusion, immunoelectrophoresis, and PAP immunohistochemistry. Sections of normal tissues from six patients and a total of 43 neoplasms were examined. Treatment of the tissue sections with trypsin prior to application of the antiserum markedly increased the sensitivity of FVIII/vWF-AG detection. The positive staining for FVIII/vWF-AG was restricted to endothelial cells in both neoplastic and nonneoplastic tissue. In general, the hyperplastic endothelia in neoplastic and reactive tissues stained more intensely than those in normal tissues. Expression of FVIII/vWF-AG by nonendothelial neoplastic cells was not observed. FVIII/vWF-AG is a reliable marker for endothelial cells.

scholarly journals The Expression of hsp-miRNA-200b-3p and -200c-3p in Human Cytomegalovirus-infected Formalin-Fixed, Paraffin-Embedded Tissues

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S358-S358
Author(s):  
Kyoung Hwa Lee ◽  
Seoyeon Min ◽  
Seul Gi Yoo ◽  
Beom Jin Lim ◽  
Jeong-Hyeon Jo ◽  
...  
Keyword(s):  
Real Time ◽  
Human Cytomegalovirus ◽  
Gene Activation ◽  
Invasive Disease ◽  
Lytic Replication ◽  
Normal Tissues ◽  
Early Protein ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Abstract Background Human cytomegalovirus (HCMV), which exist as asymptomatic latent status, can cause the tissue invasive disease through reactivation in various immunocompromised conditions. Hsp-microRNA has a specific function of post transcriptional suppression through binding with 3’ untranslated region (UTR) of mRNA. In previous study, hsp-miR-200b-3p and -200c-3p had high probability of conjugation with 3’UTR of mRNA encoded by HCMV UL 122–123 region, which translate the immediate early protein 2 (IE2) protein. IE2 (pp86) plays an essential role to initiate and regulate viral early (E) gene activation as well as propagate the subsequent steps of HCMV lytic replication. This study was aimed to evaluate whether HCMV-infected tissue had a lower expression level of hsp-miR-200b-3p and -200c-3p. Methods We had collected the formalin-fixed, paraffin-embedded tissues (FFPEs) with cytopathic pathologic findings as well as positive immunohistochemical stain (IHC) test for HCMV (N = 111). The HCMV-uninfected normal tissues (N = 77) were selected among FFPEs with neither infection nor inflammation as well as negative HCMV IHC test. We performed TaqMan® MicroRNA real-time RT-PCR to measure the expression levels of hsp-miR-200b-3p and -200c-3p and TaqMan® real-time PCR for HCMV UL83 region to measure HCMV viral load in each FFPE. We utilized the standard curves consisting of mirVanaTM miRNA mimics corresponding to each of two miRNAs, ranging from 106 to 101 copies/µL and HCMV NIBSC 09/162 strain, ranging from 5 X 106 to 5 X 101 IU/mL. Results The levels of hsp-miR-200b-3p and -200c-3p were strongly correlated with r=0.844 (P < 0.001). The expressions levels of hsp-miR-200b-3p in HCMV-infected FFPEs (log10 3.50 ± 0.13 copies/µL) were significantly lower than normal tissues (log10 5.24 ± 0.12 copies/µL of input RNA, P < 0.001). Also, HCMV-infected FFPEs were significantly lower levels of hsp-miR-200c-3p compared than normal tissues (log10 5.28 ± 0.18 vs. 7.81 ± 0.11 copies/µL of input RNA, P = 0.025). The levels of miR-200b-3p and -200c-3p had the significant inverse correlation with HCMV VL (200b-3p, spearman r=-0.392, P < 0.001 and 200c-3p, spearman r=-0.355, P < 0.001). Conclusion The low expression of hsp-miRNA-200b-3p and -200c-3p could play a pathophysiologic role of development of HCMV tissue-invasive disease. Disclosures All authors: No reported disclosures.

A Monoclonal Antibody to Human Leukocyte Common Antigen, SHL-1, and its Use for Formalin-Fixed, Paraffin-Embedded Tissues

1991 ◽  
Vol 187 (1) ◽  
pp. 96-102
Author(s):  
Seong Hoe Park ◽  
Geon Kook Lee ◽  
Young Mee Bae ◽  
Chong Jai Kim ◽  
Hyung Geun Song ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Leukocyte ◽  
Common Antigen ◽  
Leukocyte Common Antigen ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Reactivity of anti-AraC-resistant cell monoclonal antibody, YU-311, in formalin-fixed paraffin-embedded specimens of various hematopoietic disorders

1996 ◽  
Vol 20 (11-12) ◽  
pp. 953-961 ◽  
Author(s):  
Takeaki Fukuda ◽  
Toshio Kakihara ◽  
Tomoko Kamishima ◽  
Toshiyuki Yamada ◽  
Makoto Uchiyama ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Resistant Cell ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Hematopoietic Disorders ◽  
Formalin Fixed

Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues

2010 ◽  
Vol 110 (5) ◽  
pp. 1255-1261
Author(s):  
P. Scott Donover ◽  
Brian S. Wojciechowski ◽  
Rajesh Thirumaran ◽  
Vlasta Zemba-Palko ◽  
George C. Prendergast ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Human Tissues ◽  
Tissue Inhibitor ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
Sign in / Sign up

Export Citation Format

Share Document