Anti-cancer, antiproliferative activity of active anionic H2O8 oxygen solution on HCT-116 cancer cell

HCT116 cells are adherent epithelial cells derived from the human colorectal carcinoma cell line commonly used to study inflammatory responses in colonic epithelial cells. In this study, it was aimed to evaluate the effects of active anionic H2O8 oxygen solution, which is a very strong antiviral and antimicrobial agent, on HCT-116 human colorectal cancer cell line. Cell viability was determined by MTT analysis. Antiproliferative activity of the anionic H2O8 was investigated on HCT 116 (human colorectal carcinoma) cancer cells. Anionic H2O8 displayed the outstanding activities for MTT test, IC50= 9.44 for 24th hour was calculated as IC50= 11.73 for 48th hour on HCT 116 cell line. It is thought that it can serve as an agent with strong potential to be used in treatment.

Biosynthesis of Fe nanoparticles using Alhagi sparsifolia extract for the treatment of colorectal carcinoma in the in vitro condition: A pre-clinical trial study

IntroductionThe preparation and formulation of new chemotherapeutic supplements and drugs with remarkable effects for the treatment of cancer are in the priority of both developing and developed countries. Recently, iron nanoparticles have been used as modern chemotherapeutic drugs for the treatment of several cancers such as leukemia, lung cancer, breast cancer, prostate cancer, etc. In the present study, iron nanoparticles were green-synthesized using the aqueous extract of Alhagi sparsifolia leaf aqueous extract.Material and methodsThe synthesized FeNPs were characterized by analytical techniques including SEM, TEM, UV-Vis., and FT-IR. The anti-human colorectal carcinoma activity of FeNPs was evaluated using MTT assay. In the cellular and molecular part of the recent study, the treated cells with FeNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10.ResultsThe nanoparticles were formed in a spherical shape in the average size of 47.24 nm. In the antioxidant test, the IC50 of FeNPs and BHT against DPPH free radicals were 161 and 134 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of FeNPs. The IC50 of FeNPs were 250, 293, 276, and 344 µg/mL against HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10 cell lines, respectively.ConclusionsAfter clinical study, iron nanoparticles containing Alhagi sparsifolia leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

Calendula officinalis green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities

IntroductionThe biosynthesis of metal nanoparticles using medicinal plants is not only economical but also environmentally friendly as well as having miscellaneous biomedical applications. In the present study, silver nanoparticles were green-synthesized using the aqueous extract of Calendula officinalis.Material and methodsThe synthesized AgNPs@Calendula officinalis were characterized by analytical techniques including EDX, FE-SEM, XRD, UV-Vis., and FT-IR. The anti-human colorectal cancer activities of AgNPs@Calendula officinalis were evaluated using MTT assay.ResultsThe nanoparticles were formed in a spherical shape in the range of 38.05 to 75.41 nm for the particle size. On the other hand, the MTT assay was run to evaluate anti colorectal cancer activity of AgNPs@Calendula officinalis. In the cellular and molecular part of the recent study, the treated cells with AgNPs@Calendula officinalis were assessed by MTT assay for 48 h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. WiDr, SW1417 [SW-1417], and DLD-1. In the antioxidant test, the IC50 of AgNPs@Calendula officinalis and BHT against DPPH free radicals were 222 and 124 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of AgNPs@Calendula officinalis. The IC50 of AgNPs@Calendula officinalis were 430, 326, and 392 µg/mL against WiDr, SW1417 [SW-1417], and DLD-1 cell lines, respectively.ConclusionsAfter the clinical study, silver nanoparticles containing Calendula officinalis leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

Di-2-pyridylketone 4, 4-dimethyl-3-thiosemicarbazone effectively induces human colorectal carcinoma cell apoptosis via mTOR pathway

Background: To investigate the anticancer mechanisms of di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) in human colon cancer cells. Human colorectal carcinoma (HCC) is one of the most commonly diagnosed cancers in both males and females. Current studies have found that iron chelators can be used as novel anticancer drugs; however, the anticancer activity of iron chelators and their target genes in HCC has been rarely reported. Methods: Dp44mT was used to treat two colorectal tumor cell lines, SW480 and HT-29. The proapoptotic effects of different concentrations of Dp44mt were measured using flow cytometry and Hoechst 33258 staining. Ferric ammonium citrate (FAC) was used as an additional iron donor to inhibit the effects of Dp44mT. Apoptosis and DNA damage-related proteins were examined by Western blot analysis. Results: In this study, we found that the iron chelators Dp44mT could induce the apoptosis in two colorectal tumor cell lines SW480 and HT-29, upregulate the expression level of p-histone H2A.X, and inhibit the phosphorylation level of mTOR in a dose-dependent way. Those effects could be reversed by the additional iron donor FAC. Conclusion: These data indicate that iron depletion and/or the presence of iron can modulate the HCC apoptosis progression in vitro, which may be a potential target for future HCC therapy.

Antitumor Mechanism of Hydroxycamptothecin via the Metabolic Perturbation of Ribonucleotide and Deoxyribonucleotide in Human Colorectal Carcinoma Cells

Hydroxycamptothecin (SN38) is a natural plant extract isolated from Camptotheca acuminate. It has a broad spectrum of anticancer activity through inhibition of DNA topoisomerase I, which could affect DNA synthesis and lead to DNA damage. Thus, the action of SN38 against cancers could inevitably affect endogenous levels of ribonucleotide (RNs) and deoxyribonucleotide (dRNs) that play critical roles in many biological processes, especially in DNA synthesis and repair. However, the exact impact of SN38 on RNs and dRNs is yet to be fully elucidated. In this study, we evaluated the anticancer effect and associated mechanism of SN38 in human colorectal carcinoma HCT 116 cells. As a result, SN38 could decrease the cell viability and induce DNA damage in a concentration-dependent manner. Furthermore, cell cycle arrest and intracellular nucleotide metabolism were perturbed due to DNA damage response, of which ATP, UTP, dATP, and TTP may be the critical metabolites during the whole process. Combined with the expression of deoxyribonucleoside triphosphates synthesis enzymes, our results demonstrated that the alteration and imbalance of deoxyribonucleoside triphosphates caused by SN38 was mainly due to the de novo nucleotide synthesis at 24 h, and subsequently the salvage pathways at 48 h. The unique features of SN38 suggested that it might be recommended as an effective supplementary drug with an anticancer effect.