Oxygen radicals in postischaemic damages in the kidney

M. Wolgast; A. Bayati; O. Hellberg; Ö. Källskog; K. Nygren; G. Öjteg

doi:10.1007/bf01645162

Oxygen radicals participate in alloxan-induced pulmonary EDEMA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159047 ◽

1990 ◽

Vol 48 (3) ◽

pp. 298-299

Author(s):

Tomoo Kawada ◽

Michio Arakawa ◽

Kenjiro Kambara ◽

Takashi Segawa ◽

Fumio Ando ◽

...

Keyword(s):

Pulmonary Edema ◽

Oxygen Radicals ◽

Bolus Injection ◽

Physiological Saline ◽

Baseline Period ◽

Alveolar Epithelial Cells ◽

Control Group ◽

Intervention Period ◽

Lung Water ◽

Electron Microscopic

We know that alloxan causes increased-permeability pulmonary edema and that alloxan generates oxygen radicals (H2O2, O2−, ·OH) in blood. Therefore, we hypothesize that alloxan-generated oxygen radicals damage pulmonary capillary endothelial cells, and, possibly, alveolar epithelial cells as well. We examined whether oxygen radical scavengers, such as catalase or dimethylsulfoxide (DMSO), protected against alloxaninduced pulmonary edema.Five dogs in each following group were anesthetized: control group: physiological saline (20ml/kg/h); alloxan group: physiological saline + alloxan (75mg/kg) bolus injection at the beginning of the experiment; catalase group: physiological saline + catalase (150,000u/kg) bolus injection before injection of alloxan; DMSO group: physiological saline + DMSO (0.4mg/kg) bolus injection before alloxan. All dogs had 30-min baseline period and 3-h intervention period. Hemodynamics and circulating substances were measured at the specific points of time. At the end of intervention period, the dogs were killed and had the lungs removed for electron microscopic study and lung water measurement with direct destructive method.

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The Dinitrophenol and Potassium Iodate Influence on Hordeum Vulgare Seedlings Viability\

Revista de Chimie ◽

10.37358/rc.18.8.6491 ◽

2018 ◽

Vol 69 (8) ◽

pp. 2160-2166

Author(s):

Elena Todirascu Ciornea ◽

Gabriela Dumitru ◽

Ion Sandu

Keyword(s):

Oxidative Stress ◽

Hordeum Vulgare ◽

Antioxidant Enzyme ◽

Oxygen Radicals ◽

Free Oxygen Radicals ◽

Potassium Iodate ◽

Free Oxygen ◽

Oxidative Stress Enzymes ◽

Stress Enzymes ◽

Seeds Germination

The using of the pesticides of dinitrophenol type in agriculture has as consequence the major pollution of the environment, the plants taking these substances from the soil and once with these ones they reach in the human and animal organism where they product disequilibrium that are interpreted through the accumulation of free oxygen radicals with direct repercussions on the antioxidant enzyme�s synthesis intensification and on their activity�s increase. The apply of treatments on the barley seeds had significant effects regarding the seeds� germination, the young plants� growth, the oxidative stress enzymes� activity, but also regarding the content of photoassimilators and carotenoids pigments.

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Oxygen radicals trigger activation of NF-κB and AP-1 and upregulation of ICAM-1 in reperfused canine heart

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00796.2000 ◽

2002 ◽

Vol 282 (5) ◽

pp. H1778-H1786 ◽

Cited By ~ 79

Author(s):

Haiying Fan ◽

Baogui Sun ◽

Qiuping Gu ◽

Anne Lafond-Walker ◽

Suyi Cao ◽

...

Keyword(s):

Dna Binding ◽

Protein Expression ◽

Vascular Endothelium ◽

Oxygen Radicals ◽

Nuclear Dna ◽

Ischemia Reperfusion ◽

Intercellular Adhesion Molecule ◽

Radical Scavenger ◽

Myeloperoxidase Activity ◽

Canine Heart

We investigated whether oxygen radicals generated during ischemia-reperfusion trigger postischemic inflammation in the heart. Closed-chest dogs underwent 90-min coronary artery occlusion, followed by 1- or 3-h reperfusion: 10 dogs received the cell-permeant oxygen radical scavenger N-(2-mercaptopropionyl)-glycine (MPG; 8 mg · kg−1 · h−1intracoronary) beginning 5 min before reperfusion, and 9 dogs received vehicle. Blood flow (microspheres), intercellular adhesion molecule (ICAM)-1 protein expression (immunohistochemistry), ICAM-1 gene activation (Northern blotting), nuclear DNA binding activity of nuclear factor (NF)-κb and AP-1 (electrophoretic mobility shift assays), and neutrophil (PMN) accumulation (myeloperoxidase activity) were assessed in myocardial tissue samples. ICAM-1 protein expression was high in vascular endothelium after ischemia-reperfusion but was markedly reduced by MPG. MPG treatment also markedly decreased expression of ICAM-1 mRNA and tissue PMN accumulation. Nuclear DNA binding activities of NF-κB and AP-1, increased by ischemia-reperfusion, were both markedly decreased by MPG at 1 h of reperfusion. However, by 3 h, AP-1 activity was only modestly reduced by MPG and NF-κB activity was not significantly different from ischemic-reperfused controls. These results suggest that oxygen radicals generated in vivo during reperfusion trigger early activation of NF-κb and AP-1, resulting in upregulation of the ICAM-1 gene in vascular endothelium and subsequent tissue accumulation of activated PMNs.

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The participation of hydroperoxides and oxygen radicals in the control of prostaglandin synthesis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)32301-9 ◽

1983 ◽

Vol 258 (11) ◽

pp. 6855-6857 ◽

Cited By ~ 4

Author(s):

L Taylor ◽

M J Menconi ◽

P Polgar

Keyword(s):

Oxygen Radicals ◽

Prostaglandin Synthesis

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Modulation of the fibrinolytic response of cultured human vascular endothelium by extracellularly generated oxygen radicals.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)48536-5 ◽

1992 ◽

Vol 267 (1) ◽

pp. 597-601

Author(s):

M A Shatos ◽

J M Doherty ◽

T Orfeo ◽

J C Hoak ◽

D Collen ◽

...

Keyword(s):

Vascular Endothelium ◽

Oxygen Radicals

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Role of Oxidative Stress in the Pathogenesis of Amyotrophic Lateral Sclerosis: Antioxidant Metalloenzymes and Therapeutic Strategies

Biomolecules ◽

10.3390/biom11030437 ◽

2021 ◽

Vol 11 (3) ◽

pp. 437

Author(s):

Pavlína Hemerková ◽

Martin Vališ

Keyword(s):

Oxidative Stress ◽

Amyotrophic Lateral Sclerosis ◽

Antioxidant Enzymes ◽

Oxygen Radicals ◽

Clinical Symptoms ◽

Free Oxygen Radicals ◽

Free Oxygen ◽

Sporadic Als ◽

Familial Form ◽

Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) affects motor neurons in the cerebral cortex, brainstem and spinal cord and leads to death due to respiratory failure within three to five years. Although the clinical symptoms of this disease were first described in 1869 and it is the most common motor neuron disease and the most common neurodegenerative disease in middle-aged individuals, the exact etiopathogenesis of ALS remains unclear and it remains incurable. However, free oxygen radicals (i.e., molecules containing one or more free electrons) are known to contribute to the pathogenesis of this disease as they very readily bind intracellular structures, leading to functional impairment. Antioxidant enzymes, which are often metalloenzymes, inactivate free oxygen radicals by converting them into a less harmful substance. One of the most important antioxidant enzymes is Cu2+Zn2+ superoxide dismutase (SOD1), which is mutated in 20% of cases of the familial form of ALS (fALS) and up to 7% of sporadic ALS (sALS) cases. In addition, the proper functioning of catalase and glutathione peroxidase (GPx) is essential for antioxidant protection. In this review article, we focus on the mechanisms through which these enzymes are involved in the antioxidant response to oxidative stress and thus the pathogenesis of ALS and their potential as therapeutic targets.

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