Effect of levamisole on lysosomal enzyme release from polymorphonuclear leukocytes and intracellular levels of cAMP and cGMP after phagocytosis of monosodium urate crystals

Vatarakta is more distressing and common metabolic disorder prevalent in present era. It is Vatapradhana Tridoshaja Vatavyadhi where Rakta is main Dushya. Vata is a predominant Dosha in Vatarakta, when it is excessively aggravated with vitiated Rakta is called Vatarakta. Vatarakta is correlated with gout in modern science. Gout is an abnormality of purine metabolism causes hyperuricemia and deposition of monosodium urate crystals in joints. Pain is predominant symptom of gout, which disturbs day-today life of the patients. The Panchakarma is not only a important component of Ayurvedic treatment but it is also forms the fundamental basis of Ayurveda therapy. The different procedures like Swedana, Vamana, Virechana, Basti, Sirovirechana, Raktamokshana focus on the purification which detoxifies the human body, i.e. the correction of the metabolism at molecular level. These detoxification procedures are essential components of the management in various metabolic disorders. So Panchakarma is an unique approach in the management of Vatarakta.

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Anti-Hyperuricemic Effect of Iron Oxide Nanoparticles against Monosodium Urate Crystals Induced Gouty Arthritis in BALB/c Mice

Biological Trace Element Research ◽

10.1007/s12011-021-02769-0 ◽

2021 ◽

Author(s):

Mubin Mustafa Kiyani ◽

Nurain Baig Moghul ◽

Maisra Azhar Butt ◽

Hamza Rehman ◽

Ramsha Masood ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Gouty Arthritis ◽

Oxide Nanoparticles ◽

Monosodium Urate ◽

Monosodium Urate Crystals ◽

Urate Crystals

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Pertussis toxin inhibition of chemotactic factor-induced calcium mobilization and function in human polymorphonuclear leukocytes.

Journal of Experimental Medicine ◽

10.1084/jem.162.1.145 ◽

1985 ◽

Vol 162 (1) ◽

pp. 145-156 ◽

Cited By ~ 119

Author(s):

D W Goldman ◽

F H Chang ◽

L A Gifford ◽

E J Goetzl ◽

H R Bourne

Keyword(s):

Pertussis Toxin ◽

Lysosomal Enzyme ◽

Polymorphonuclear Leukocytes ◽

Regulatory Protein ◽

Leukotriene B4 ◽

Ionophore A23187 ◽

Enzyme Release ◽

Adp Ribosylation ◽

Chemotactic Factors ◽

Human Polymorphonuclear Leukocytes

Chemotactic factors stimulate a rapid increase in the cytosolic concentration of intracellular calcium ions ([Ca2+]in) in human polymorphonuclear leukocytes (PMNL), which may be an event that is critical to the expression of chemotaxis and other PMNL functions. Treatment of PMNL with pertussis toxin catalyzes ADP-ribosylation of a protein similar or identical to the inhibiting regulatory protein of adenylate cyclase, Gi, and suppresses the increase in [Ca2+]in elicited by leukotriene B4(LTB4) and formyl-methionyl-leucyl-phenylalanine. Chemotactic migration and lysosomal enzyme release elicited by chemotactic factors were inhibited by pertussis toxin with a concentration-dependence similar to that for inhibition of the increase in [Ca2+]in, without an effect on lysosomal enzyme release induced by the ionophore A23187 and phorbol myristate acetate. Activated pertussis toxin catalyzed the [32P]ADP-ribosylation of a 41 kD protein in homogenates of PMNL. The extent of [32P]ADP-ribosylation of this protein was reduced 59% by pretreatment of intact PMNL with pertussis toxin. Pertussis toxin selectively decreased the number of high-affinity receptors for LTB4 on PMNL by 60% without altering the number or binding properties of the low-affinity subset of receptors. Pertussis toxin modification of a membrane protein of PMNL analogous to Gi thus simultaneously alters chemotactic receptors and attenuates the changes in cytosolic calcium concentration and PMNL function caused by chemotactic factors.

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