scholarly journals Expression of a bovine κ-CN cDNA in the mammary gland of transgenic mice utilizing a genomic milk protein gene as an expression cassette

1996 ◽  
Vol 5 (4) ◽  
pp. 271-279 ◽  
Author(s):  
Alfonso Gutiérrez ◽  
Harry M. Meade ◽  
Paul Ditullio ◽  
Daniel Pollock ◽  
Merry Harvey ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Transgenic Mice ◽  
Milk Protein ◽  
Protein Gene ◽  
Expression Cassette ◽  
Milk Protein Gene

scholarly journals Nuclear Factor 1-C2 Contributes to the Tissue-specific Activation of a Milk Protein Gene in the Differentiating Mammary Gland

2002 ◽  
Vol 277 (20) ◽  
pp. 17589-17596 ◽  
Author(s):  
Marie Kannius-Janson ◽  
Eva M. Johansson ◽  
Gunnar Bjursell ◽  
Jeanette Nilsson
Keyword(s):  
Mammary Gland ◽  
Milk Protein ◽  
Protein Gene ◽  
Nuclear Factor ◽  
Milk Protein Gene ◽  
Tissue Specific ◽  
Nuclear Factor 1

scholarly journals Targeted c-myc gene expression in mammary glands of transgenic mice induces mammary tumours with constitutive milk protein gene transcription.

1988 ◽  
Vol 7 (1) ◽  
pp. 169-175 ◽  
Author(s):  
C. A. Schoenenberger ◽  
A. C. Andres ◽  
B. Groner ◽  
M. van der Valk ◽  
M. LeMeur ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Gene Transcription ◽  
Milk Protein ◽  
Protein Gene ◽  
Mammary Glands ◽  
Milk Protein Gene ◽  
Mammary Tumours ◽  
Myc Gene

scholarly journals Suppression of lactation and acceleration of involution in the bovine mammary gland by a selective serotonin reuptake inhibitor

2011 ◽  
Vol 209 (1) ◽  
pp. 45-54 ◽  
Author(s):  
L L Hernandez ◽  
J L Collier ◽  
A J Vomachka ◽  
R J Collier ◽  
N D Horseman
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Milk Yield ◽  
Milk Protein ◽  
Protein Gene ◽  
Holstein Cows ◽  
Dependent Manner ◽  
Bovine Mammary Gland ◽  
Milk Protein Gene ◽  
Milk Protein Gene Expression

Serotonin (5-HT) is a homeostatic regulator of lactation. Selective 5-HT reuptake inhibitors (SSRI) are commonly prescribed pharmaceuticals that inhibit activity of the 5-HT reuptake transporter, increasing cellular exposure to 5-HT. Use of SSRIs has been shown to alter lactation performance in humans and 5-HT has been shown to reduce milk yield in cattle. However, it has not been determined how SSRI treatments affect the bovine mammary gland. We evaluated the effects of SSRI (fluoxetine (FLX)) administration on tight junctions (TJs) and milk protein gene expression in a lactogenic culture model, using primary bovine mammary epithelial cells (pBMEC). Additionally, we evaluated the effects of intramammary infusions of FLX and 5-hydroxytryptophan on milk production and TJ status in multiparous Holstein cows at dry-off. Treatment of pBMEC cultured on permeable membranes disrupted TJs, as measured by transepithelial resistance and immunostaining for zona occludens 1. Correspondingly, treatment of ‘3D’, collagen-embedded lactogenic cultures of pBMEC with FLX suppressed milk protein gene expression (α-lactalbumin and β-casein) in a concentration-dependent manner. Finally, intramammary treatment of Holstein cows with FLX resulted in an accelerated rate of milk decline. Additionally, TJ permeability increased in FLX-treated animals, as measured by plasma lactose and milk Na+ and K+ levels. Results of these experiments imply that SSRI administration accelerates the rate of mammary gland involution through disassembly of TJs and inhibition of milk protein gene expression in vitro and in vivo, leading to reduction of milk yield.

scholarly journals The effect of growth hormone on milk protein gene expression in the bovine mammary gland

2004 ◽  
Vol 13 (Suppl. 1) ◽  
pp. 437-440 ◽  
Author(s):  
S. McCoard ◽  
N. Roy ◽  
B. Sinclair ◽  
M. Deighton ◽  
W. McNabb
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Mammary Gland ◽  
Milk Protein ◽  
Protein Gene ◽  
Bovine Mammary Gland ◽  
Milk Protein Gene ◽  
Milk Protein Gene Expression
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