Cholesterol in amniotic fluid, maternal and umbilical cord blood

1978 ◽  
Vol 226 (4) ◽  
pp. 289-296 ◽  
Author(s):  
A. E. Schindler ◽  
M. Anderer ◽  
H. M. Liebich
1989 ◽  
Vol 27 (3) ◽  
pp. 137-142 ◽  
Author(s):  
Ralph J. Lellé ◽  
Eberhard Henkel ◽  
Dieter Leinemann ◽  
Klaus Goeschen

Reproduction ◽  
2012 ◽  
Vol 143 (4) ◽  
pp. 455-468 ◽  
Author(s):  
Eleonora Iacono ◽  
Lara Brunori ◽  
Alessandro Pirrone ◽  
Pasquale Paolo Pagliaro ◽  
Francesca Ricci ◽  
...  

Mesenchymal stem cells (MSCs) have been derived from multiple sources of the horse including umbilical cord blood (UCB) and amnion. This work aimed to identify and characterize stem cells from equine amniotic fluid (AF), CB and Wharton's Jelly (WJ). Samples were obtained from 13 mares at labour. AF and CB cells were isolated by centrifugation, while WJ was prepared by incubating with an enzymatic solution for 2 h. All cell lines were cultured in DMEM/TCM199 plus fetal bovine serum. Fibroblast-like cells were observed in 7/10 (70%) AF, 6/8 (75%) CB and 8/12 (66.7%) WJ samples. Statistically significant differences were found between cell-doubling times (DTs): cells isolated from WJ expanded more rapidly (2.0±0.6 days) than those isolated from CB (2.6±1.3 days) and AF (2.3±1.0 days) (P<0.05). Positive von Kossa and Alizarin Red S staining confirmed osteogenesis. Alcian Blue staining of matrix glycosaminoglycans illustrated chondrogenesis and positive Oil Red O lipid droplets staining suggested adipogenesis. All cell lines isolated were positive for CD90, CD44, CD105; and negative for CD34, CD14 and CD45. These findings suggest that equine MSCs from AF, UCB and WJ appeared to be a readily obtainable and highly proliferative cell lines from a uninvasive source that may represent a good model system for stem cell biology and cellular therapy applications in horses. However, to assess their use as an allogenic cell source, further studies are needed for evaluating the expression of markers related to cell immunogenicity.


2007 ◽  
Vol 23 (5) ◽  
pp. 295-299 ◽  
Author(s):  
Jacek Zamłyński ◽  
Jerzy Chudek ◽  
Anita Olejek ◽  
Barbara Kobylec-Zamłyńska ◽  
Grzegorz Mańka ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document