Clinical significance of antibodies to double stranded DNA (dsDNA) for systemic lupus erythematosus (SLE)

Objective. To investigate the expression and clinical significance of trans-membrane MerTK (mMer) on circulating CD14+ monocytes/macrophages and soluble MerTK (sMer) levels in plasma in systemic lupus erythematosus (SLE).Method. 108 SLE patients and 42 healthy controls were recruited in this study. The expression of mMer on the surfaces of CD14+ monocytes/macrophages was evaluated by flow cytometry (FCM). The sMer levels were measured by ELISA. Real-time quantitative PCR was applied to evaluate the mRNA levels of MerTK and ADAM17.Results. Both mMer expression on CD14+ monocytes/macrophages and sMer levels in plasma significantly increased in SLE patients compared to healthy subjects. The frequency of anti-inflammatory MerTK expressing CD14+CD16+ monocytes decreased in SLE. mMer expression was positively correlated with CD163 expression on CD14+ cells. Both the mMer expression on CD14+ monocytes/macrophages and sMer levels in plasma were positively correlated with SLEDAI. Furthermore, more elevated mMer and sMer levels were found in patients with higher SLEDAI, presence of anti-SSA, anti-Sm autoantibodies, and lupus nephritis.Conclusion. Both mMer and sMer levels significantly increased in SLE and positively correlated with disease activity and severity. The upregulation of MerTK expression may serve as a biomarker of the disease activity and severity of SLE.

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Clinical Significance of Autoantibodies in Systemic Lupus Erythematosus

Rheumatology ◽

10.1093/rheumatology/33.12.1202 ◽

1994 ◽

Vol 33 (12) ◽

pp. 1202-1203 ◽

Cited By ~ 3

Author(s):

M. HIETARINTA ◽

M. VIANDER ◽

A. TOIVANEN ◽

O. LASSILA

Keyword(s):

Systemic Lupus Erythematosus ◽

Clinical Significance ◽

Lupus Erythematosus ◽

Systemic Lupus

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Inhibition of Adenovirus DNA Synthesis In Vitro by Sera from Patients with Systemic Lupus Erythematosus

Molecular and Cellular Biology ◽

10.1128/mcb.2.12.1492-1500.1982 ◽

1982 ◽

Vol 2 (12) ◽

pp. 1492-1500

Author(s):

Marshall S. Horwitz ◽

Beth R. Friefeld ◽

Harold D. Keiser

Keyword(s):

Systemic Lupus Erythematosus ◽

Dna Synthesis ◽

Lupus Erythematosus ◽

Inhibitory Activity ◽

Antinuclear Antibodies ◽

Dna Binding Protein ◽

Systemic Lupus ◽

Double Stranded Dna ◽

The Individual

Sera containing antinuclear antibodies from patients with systemic lupus erythematosus (SLE) and related disorders were tested for their effect on the synthesis of adenovirus (Ad) DNA in an in vitro replication system. After being heated at 60°C for 1 h, some sera from patients with SLE inhibited Ad DNA synthesis by 60 to 100%. Antibodies to double-stranded DNA were present in 15 of the 16 inhibitory sera, and inhibitory activity copurified with anti-double-stranded DNA in the immunoglobulin G fraction. These SLE sera did not inhibit the DNA polymerases α, β, γ and had no antibody to the 72,000-dalton DNA-binding protein necessary for Ad DNA synthesis. The presence of antibodies to single-stranded DNA and a variety of saline-extractable antigens (Sm, Ha, nRNP, and rRNP) did not correlate with SLE serum inhibitory activity. Methods previously developed for studying the individual steps in Ad DNA replication were used to determine the site of inhibition by the SLE sera that contained antibody to double-stranded DNA. Concentrations of the SLE inhibitor that decreased the elongation of Ad DNA by greater than 85% had no effect on either the initiation of Ad DNA synthesis or the polymerization of the first 26 deoxyribonucleotides.

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AB0567 Clinical significance of the detection of hla-drb1 alleles in patients with systemic lupus erythematosus

10.1136/annrheumdis-2018-eular.4737 ◽

2018 ◽

Author(s):

E. Belolipetskaia ◽

I. Beliaeva ◽

V. Mazurov ◽

S. Lapin ◽

O. Tkachenko ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Clinical Significance ◽

Lupus Erythematosus ◽

Systemic Lupus

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AB0638 Clinical significance of esr in chinese patients with systemic lupus erythematosus

10.1136/annrheumdis-2018-eular.3378 ◽

2018 ◽

Author(s):

Y. Xu ◽

X. Feng ◽

L. Sun ◽

Keyword(s):

Systemic Lupus Erythematosus ◽

Clinical Significance ◽

Lupus Erythematosus ◽

Chinese Patients ◽

Systemic Lupus

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Correlation between Osteoprotegerin Levels and Antiphospholipid Syndrome Parameters

Laboratory Medicine ◽

10.1093/labmed/lmz051 ◽

2019 ◽

Author(s):

Simona Caraiola ◽

Alina Dima ◽

Ciprian Jurcut ◽

Ruxandra Jurcut ◽

Cristian Baicus ◽

...

Keyword(s):

Risk Factors ◽

Systemic Lupus Erythematosus ◽

Antiphospholipid Syndrome ◽

Lupus Erythematosus ◽

Antiphospholipid Antibodies ◽

High Serum ◽

Systemic Lupus ◽

Clinical Events ◽

Double Stranded Dna ◽

Female Data

Abstract Objective To identify the osteoprotegerin (OPG) correlates with antiphospholipid syndrome (APS) parameters. Methods Our cohort included 40 patients with primary APS disease associated with systemic lupus erythematosus (SLE) (mean age, 43.7 years; 87% female). Data on cardiovascular risk factors and specific clinical events in APS were collected. Then we tested OPG and 10 criteria and noncriteria antiphospholipid antibodies (aPLs) on preserved specimens in all cases. Results A total of 26 patients (65%) had high serum OPG levels. Patients with high OPG were mostly overweight. In patients with SLE, the OPG levels were associated with anti–double-stranded DNA (anti-dsDNA) and anti-Sm titers. However, we did not find significant correlations of the OPG with any of the 10 aPLs tested. Also, we found no relationship regarding venous APS events. Conclusion In APS, high OPG levels are not linked to serum aPL expression.

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