Microbial populations in trifluralin-treated soil

A study was carried out in six beet sugar factories in France during the 2012/13 sugar campaign. The objective was to assess the optimal dosage of formaldehyde solutions at specific process stages and in different existing factory set-ups in order to obtain the desired effect on microbial populations, without interference with the quality of the products. In addition harmlessness regarding consumer health was to be demonstrated. A series of experiments was conducted resulting in new data allowing refreshment of common knowledge and references existing regarding the use of formaldehyde solutions in the sugar industry. The effectiveness and convenience for controlling microbiological activity in beet sugar manufacture was assessed. Formaldehyde reduces sugar losses and protects in-process products without harming their further use, such as for ethanol production.

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Isolation of Epigallocatechin Gallate from Green Tea and its Effects on Probiotics and Pathogenic Bacteria

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.17:69-77 ◽

2017 ◽

Vol 17 (1) ◽

pp. 69-77

Author(s):

Tu Lijun ◽

Sun Hanju ◽

He Shudong ◽

Zhu Yongsheng ◽

Yu Ming ◽

...

Keyword(s):

Mass Spectrometry ◽

Escherichia Coli ◽

Green Tea ◽

Lactobacillus Acidophilus ◽

Pathogenic Bacteria ◽

Epigallocatechin Gallate ◽

Microbial Populations ◽

Medium Ph ◽

Bioactive Substance ◽

Tea Extract

The aim of this study was to investigate epigallocatechin gallate (EGCG) prebiotics activities systematically which was reported as a bioactive substance. Therefore, EGCG was separated by water extraction, resin purification and prep-HPLC. Then the production of EGCG was confirmed by HPLC and mass spectrometry (MS) analysis and its purify was 97.23%. EGCG extractive and green tea extract (GTE) were further incubated with Bifidobacterium infantis, B. adolescentis, B. bifidum and Lactobacillus acidophilus to study its effect on microbial populations and medium pH. Finally, Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans were employed as pathogenic bacteria to explore the antimicrobial activity of EGCG and GTE. The results demonstrated that EGCG extractive could be beneficial for the proliferation of Bifidobacterium and L. acidophilus and also inhibit some pathogenic bacteria. In conclusion, both EGCG extractive and GTE had prebiotics activities and the effects of EGCG extractive were superior to those of GTE.

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Using microbial fatty acids to quantify, characterize and compare biofilm and suspended microbial populations in wastewater treatment systems

Water Science & Technology ◽

10.2166/wst.1998.0644 ◽

1998 ◽

Vol 38 (4-5) ◽

pp. 273-280 ◽

Cited By ~ 3

Author(s):

A. G. Werker ◽

E. R. Hall

Keyword(s):

Fatty Acid ◽

Methyl Esters ◽

Ph Control ◽

Microbial Populations ◽

Total Organic Carbon Removal ◽

Operating Limits ◽

Suspended Biomass ◽

Kraft Mill Effluent ◽

Microbial Fatty Acid ◽

Influence Of Ph

Spectra of microbial fatty acid methyl esters were used to assess the influence of pH on the biofilm and suspended biomass populations within bench scale moving bed bioreactors treating bleached kraft mill effluent. The fatty acid spectral data were reduced by logcontrast canonical component analysis. The first canonical component differentiated biofilm from suspended populations and the second two appeared to indicate an influence of pH on microbial ecology. Thus, the biofilm and suspended microbial communities were distinct, as were the populations acclimated at pH 6 and pH 8. Experimental data for total organic carbon removal suggested that the more recalcitrant fraction of the influent was consumed by the slower growing, high SRT biofilm fraction of the biomass. Since the biofilm ecology was sensitive to pH within typical pH operating limits of full-scale systems, tighter pH control might improve treatment reliability in the event of process transients.

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Bio-P and non-bio-P bacteria identification by a novel microbial approach

Water Science & Technology ◽

10.2166/wst.1999.0249 ◽

1999 ◽

Vol 39 (6) ◽

pp. 13-20 ◽

Cited By ~ 6

Author(s):

Philip L. Bond ◽

Jürg Keller ◽

Linda L. Blackall

Keyword(s):

In Situ Hybridisation ◽

Microbial Populations ◽

Biological Phosphorus Removal ◽

Gram Positive Bacteria ◽

P Content ◽

Identification Of Bacteria ◽

Widespread Belief ◽

Biological Phosphorus ◽

P Removal

Culturing bacteria from activated sludge with enhanced biological phosphorus removal (EBPR) has strongly implicated Acinetobacter with the process. However, using fluorescent in-situ hybridisation (FISH) probing to analyse microbial populations, we have shown evidence opposing this widespread belief. We describe the phosphorus (P) removing performance and microbial population analyses of sludges obtained in a laboratory scale EBPR reactor. Two sludges with extremely high P removing capabilities were examined, the P content of these sludges was 8.6% (P sludge) and 12.3% (S sludge) of the MLSS. Identification of bacteria using FISH probing indicated both sludges were dominated by microbes from the beta proteobacteria and high mol% G+C Gram positive bacteria. Acinetobacter could make up only a small proportion of the cells in these sludges. Sludge with extremely poor P removal (P content of 1.5%, referred to as T sludge) was then generated by reducing the P in the influent. Bacteria resembling the G-bacteria became abundant in this sludge and these were identified using FISH probing. The anaerobic transformations of the T and P sludges correlated well with that of the non-EBPR and EBPR biological models respectively, indicating that bacteria in the T sludge have the potential to inhibit P removal in EBPR systems.

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