Candidate reference method: Determination of valproic acid in serum by isotope-dilution mass spectrometry

1995 ◽  
Vol 41 (7-8) ◽  
pp. 407-413 ◽  
Author(s):  
F. Susanto ◽  
H. Reinauer
Keyword(s):  
Mass Spectrometry ◽  
Valproic Acid ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Reference Method ◽  
Valproic Acid In Serum

Effect of detergent on kinetic Jaffé-method assay of creatinine.

1980 ◽  
Vol 26 (12) ◽  
pp. 1729-1732 ◽  
Author(s):  
A Bergman ◽  
G Ohman
Keyword(s):  
Mass Spectrometry ◽  
Serum Creatinine ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Reference Method

Abstract Using isotope dilution-mass spectrometry as reference method, we show that the kinetic Jaffé method for determination of serum creatinine sometimes produces results that are much too high. Such falsely increased values were obtained in about 10% of the sera collected for routine determination of creatinine, and were most common when certain types of collecting tubes were used. The interferences could be overcome by use of detergent in the reaction mixture.

Determination of serum cholesterol by isotope dilution mass spectrometry with a benchtop capillary gas chromatograph/mass spectrometer: comparison with the National Reference System's Definitive and Reference Methods

1991 ◽  
Vol 37 (7) ◽  
pp. 1161-1165 ◽  
Author(s):  
John H Eckfeldt ◽  
Linda A Lewis ◽  
John D Belcher ◽  
Jasbir Singh ◽  
Ivan D Frantz
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometer ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Internal Standard ◽  
Reference Method ◽  
Gas Chromatograph ◽  
National Reference ◽  
Definitive Methods

Abstract We developed an isotope dilution mass spectrometric cholesterol method with [25,26,27-13C]cholesterol as internal standard and a benchtop gas chromatograph/mass spectrometer (GC/MS) that is much easier and less time consuming than previously described Reference and Definitive Methods for cholesterol. The internal standard, cholesterol standards, and unknown specimen are delivered volumetrically with an automated dilutor and the saponifying reagent. After saponification, extraction, and derivatization, specimens are injected into a benchtop quadrupole MS with an autosampler. Unknown cholesterol concentrations are calculated automatically by comparing the peak area ratio of the m/z = 368, 371 ion pair with the ratios for the cholesterol standards (0 to 12.93 mmol/L). We found within-run and day-to-day (overall) imprecision of 0.44% and 0.95%, respectively, when specimens were assayed singly. In several lyophilized and frozen Standard Reference Material (SRM) pools, cholesterol results with our GC/MS method averaged 0.4% less than the National Institute for Standards and Technology definitive GC/MS result performed about three years earlier. Our GC/MS results averaged 1.3% and 2.0% less than results by the National Reference System (NRS) Abell-Levy-Brodie-Kendall (ALBK) results from clinical specimens and the SRM pools, respectively. These results are consistent with the previously reported bias between the NRS Reference and Definitive Methods and the 0.1% per year decrease in cholesterol concentrations in SRM pools as determined by GC/MS analysis. These results further emphasize the small but consistent bias between cholesterol results by isotope dilution mass spectrometry and the ALBK Reference Method, the latter being the basis for the National Cholesterol Education Program guidelines and population reference values.

Quantification of ferritin bound iron in human serum using species-specific isotope dilution mass spectrometry

Metallomics ◽  
2014 ◽  
Vol 6 (9) ◽  
pp. 1709-1717 ◽  
Author(s):  
Yao Ren ◽  
Thomas Walczyk
Keyword(s):  
Mass Spectrometry ◽  
Human Serum ◽  
Isotope Dilution ◽  
Iron Status ◽  
Isotope Dilution Mass Spectrometry ◽  
Reference Method ◽  
Precise Determination ◽  
Body Iron ◽  
Species Specific

A potential reference method for the accurate and precise determination of ferritin bound iron as a new biomarker for body iron status.

Determination of cyanuric acid using exact matching isotope dilution mass spectrometry

2013 ◽  
Vol 36 (6) ◽  
pp. 1054-1060
Author(s):  
Guiwan Koh ◽  
Ee-mei Gui ◽  
Tang-Lin Teo ◽  
Tong-Kooi Lee
Keyword(s):  
Mass Spectrometry ◽  
Isotope Dilution ◽  
Cyanuric Acid ◽  
Isotope Dilution Mass Spectrometry ◽  
Exact Matching
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