Molecular cloning and nucleotide sequence of the coat protein gene of a Cuban isolate of potato leafroll virus and its expression inEscherichia coli

Virus Genes ◽  
1994 ◽  
Vol 9 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Lisset López ◽  
Rita Muller ◽  
Ezequiel Balmori ◽  
Gustavo de la Riva ◽  
Nadia Ramírez ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Molecular Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Inescherichia Coli ◽  
Expression Inescherichia Coli ◽  
Leafroll Virus

scholarly journals Nucleotide sequence of the potato leafroll virus coat protein gene

1989 ◽  
Vol 17 (4) ◽  
pp. 1768-1768 ◽  
Author(s):  
B. Prill ◽  
E. Maiss ◽  
U. Timpe ◽  
R. Casper
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Leafroll Virus

scholarly journals Molecular cloning and nucleotide sequence of coat protein gene of turnip mosaic virus

1990 ◽  
Vol 18 (18) ◽  
pp. 5555-5555 ◽  
Author(s):  
Ling-Jie Kong ◽  
Rong-Xiang Fang ◽  
Zheng-Hua Chen ◽  
Ke-Qiang Mang
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Turnip Mosaic Virus

scholarly journals Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids.

2005 ◽  
Vol 52 (3) ◽  
pp. 699-702 ◽  
Author(s):  
Elzbieta Sułuja ◽  
Ludmiła Strokowskaja ◽  
Włodzimierz Zagórski-Ostoja ◽  
Andrzej Pałucha
Keyword(s):  
Coat Protein ◽  
Nucleic Acids ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Recombinant Baculovirus ◽  
Potato Leafroll Virus ◽  
Rnase A ◽  
Virus Like Particles ◽  
Leafroll Virus

Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.

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