Reducing the spread of potato leaf roll virus, alfalfa mosaic virus and potato virus Y in seed potatoes by trapping aphids on sticky yellow polyethylene sheets

1979 ◽  
Vol 22 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Sara Zimmerman-Gries
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Leaf Roll ◽  
Alfalfa Mosaic Virus ◽  
Potato Leaf Roll Virus ◽  
Potato Leaf ◽  
Seed Potatoes

Biodiversity and full genome sequence of potato viruses Alfalfa mosaic virus and potato leaf roll virus in Egypt

2018 ◽  
Vol 73 (11-12) ◽  
pp. 423-438 ◽  
Author(s):  
Engy E. Abdel Aleem ◽  
Radwa M. Taha ◽  
Faiza A. Fattouh
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Leaf Roll ◽  
Alfalfa Mosaic Virus ◽  
Potato Leaf Roll Virus ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Potato Viruses

Abstract Solanum tuberosum (potato) is the second most important vegetable crop in Egypt. It is locally consumed, manufactured or supplied for export to Europe and other Arab countries. Potato is subject to infection by a number of plant viruses, which affect its yield and quality. Potato virus Y (PVY), potato leaf roll virus (PLRV), and Alfalfa mosaic virus (AMV) were detected in major potato-growing areas surveyed. Multiplex-RT-PCR assay was used for the detection of these three viruses in one reaction using three specific primer pairs designed to amplify genomic parts of each virus (1594 bp for PLRV, 795 bp for AMV, 801 bp for PVY). All three viruses were detected in a single reaction mixture in naturally infected field-grown potatoes. Multiplex RT-PCR improved sensitivity necessary for the early detection of infection. Incidence of single, double, or triple infection has been recorded in some locations. Full-length sequencing has been performed for an Egyptian FER isolate of PLRV. Through phylogenetic analysis, it was shown to occupy the same clade with isolate JokerMV10 from Germany. Complete nucleotide sequence of an Egyptian FER isolate of AMV and phylogenetic analysis was also performed; we propose that it is a new distinct strain of AMV belonging to a new subgroup IIC. This is the first complete nucleotide sequence of an Egyptian isolate of AMV. Genetic biodiversity of devastating potato viruses necessitates continuous monitoring of new genetic variants of such viruses.

scholarly journals Eliminating Potato Virus Y (PVY) and Potato Leaf Roll Virus (PLRV) Using Cryotherapy of in vitro-grown Potato Shoot Tips

2014 ◽  
Vol 59 (4) ◽  
pp. 498-504 ◽  
Author(s):  
Jung-Yoon Yi ◽  
◽  
Gi-An Lee ◽  
Jong-Wook Jeong ◽  
Sok-Young Lee ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Leaf Roll ◽  
Shoot Tips ◽  
Potato Leaf Roll Virus ◽  
Potato Leaf

scholarly journals Field Evaluation and Enzyme-Linked Immunosorbent Assay Detection of Potato Leaf Roll Virus, Potato Virus X and Potato Virus Y in Potato Germplasm

2017 ◽  
Vol 9 (7) ◽  
pp. 229 ◽  
Author(s):  
Romana Anjum ◽  
M. Aslam Khan ◽  
Kolawole Oluwaseun Olawale ◽  
Raheel Baber
Keyword(s):  
Immunosorbent Assay ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Rating Scale ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Leaf Roll Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Potato Varieties

Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.

scholarly journals Yield and starch value of potatoes infected with potato leaf roll virus and potato virus Y.

1984 ◽  
Vol 30 ◽  
pp. 15-17
Author(s):  
Shingo ORITA ◽  
Takao TOMIO ◽  
Hiroshi HIROTANI
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Leaf Roll ◽  
Potato Leaf Roll Virus ◽  
Potato Leaf

scholarly journals Ocorrência de vírus em batata em sete estados do Brasil

2009 ◽  
Vol 27 (4) ◽  
pp. 490-497 ◽  
Author(s):  
Antônio Carlos de Ávila ◽  
Paulo Eduardo de Melo ◽  
Lindolfo R Leite ◽  
Alice K Inoue-Nagata
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Leaf Roll Virus ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Das Elisa

As viroses causam rápida degenerescência dos tubérculos-sementes de batata. Em condições tropicais, em que a presença de afídeos vetores é constante e a estrutura das populações de vírus é dinâmica, a pressão das doenças é enorme. Conhecer essa dinâmica é uma ferramenta importante para a sustentabilidade da produção de batata. Realizou-se um levantamento abrangente da ocorrência de viroses em batata no Brasil, além de estudar-se a distribuição das estirpes de Potato virus Y (PVY) associadas ao mosaico da batata. Em 2005 e 2006 foram visitadas lavouras em sete estados brasileiros, coletando-se folíolos com sintomas de viroses (1.256 amostras) e amostras aleatórias (360 amostras). Foi feita também uma estimativa visual da incidência de mosaico e enrolamento-das-folhas em vários dos campos visitados. Das 1.256 amostras suspeitas, 840 apresentaram reação positiva em teste sorológico para PVY (66,9%), 128 para Potato leaf roll virus (PLRV) (10,2%), 79 para Potato virus S (PVS) (6,3%) e nenhuma para Potato virus X (PVX). Os resultados dos testes de detecção por DAS-ELISA, biológico e RT-PCR mostraram a presença quase absoluta do subgrupo necrótico de PVY, em sua maioria PVY NTN. A análise de uma sub-amostragem em todos os municípios visitados confirmou que essa variante está hoje presente nos sete estados visitados. Amostras de PVY NTN foram obtidas das cultivares Asterix, Atlantic, Agata, Achat, Baronesa, Baraka, Bintje, Caesar, Cupido, Marijke, Monalisa, Panda e Vivaldi, que apresentaram diferentes níveis de suscetibilidade. As amostras aleatórias revelaram um quadro muito similar ao encontrado com as amostras sintomáticas. PLRV foi identificado em MG, BA, PR e SC, em várias lavouras de forma muito freqüente. PVS foi identificado nesses mesmos estados e também em SP. PVX foi detectado em apenas uma amostra tomada ao acaso em Serra do Salitre (MG). O contraste entre a avaliação visual dos sintomas e os resultados do teste de detecção por ELISA revelou a possibilidade de infecção latente por PVY em níveis relevantes na cultivar Asterix.

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