Nerve growth factor accelerates regeneration of cultured adult sympathetic ganglion cells

AGE ◽  
1985 ◽  
Vol 8 (1) ◽  
pp. 19-20 ◽  
Author(s):  
Y. Uchida ◽  
M. Tomonaga
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sympathetic Ganglion ◽  
Ganglion Cells ◽  
Nerve Growth

scholarly journals Effect of nerve growth factor on the release of inflammatory mediators by mature human basophils

Blood ◽  
1992 ◽  
Vol 79 (10) ◽  
pp. 2662-2669 ◽  
Author(s):  
SC Bischoff ◽  
CA Dahinden
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Inflammatory Mediators ◽  
Ganglion Cells ◽  
Neuronal Cell ◽  
Cell Types ◽  
Cell Fractionation ◽  
Hematopoietic Growth Factors ◽  
Nerve Growth ◽  
Human Basophils

Abstract Nerve growth factor (NGF) is a neurotrophic cytokine known to regulate the survival and function of peripheral and central neuronal cells. Recently, the spectrum of action could be extended to non-neuronal cell types such as rat mast cells and human B lymphocytes. The present study shows that NGF affects the function of mature human basophils isolated from the peripheral blood of healthy donors. Both murine NGF 7S and recombinant human NGF beta enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. This priming effect of NGF on basophils occurs rapidly within 10 to 15 minutes of preincubation, is dose-dependent, and requires similarly low concentrations (1 to 40 pmol/L) of human NGF beta as the induction of neurite outgrowth in ganglion cells. Cell fractionation studies indicate that NGF acts directly on human basophils without an involvement of other cell types, suggesting the presence of high-affinity NGF receptors on basophils. NGF by itself (up to 4 nmol/L of human NGF beta) does not induce the release of inflammatory mediators directly. The effect of human NGF on basophil mediator release is similar to that of the hematopoietic growth factors interleukin-3, interleukin-5, and granulocyte-macrophage colony- stimulating factor. The present study further demonstrates that NGF acts as a pleiotropic cytokine at the interface between the nervous and the immune system, and that NGF may be involved in inflammatory processes and hypersensitivity reactions.

Some characteristics of chick embryo sympathetic ganglion fragments in a simple culture system with and without nerve growth factor

1972 ◽  
Vol 138 (2) ◽  
pp. 127-133 ◽  
Author(s):  
D. R. Mottram ◽  
R. Presley ◽  
J. D. Lever ◽  
C. Ivens
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Chick Embryo ◽  
Culture System ◽  
Sympathetic Ganglion ◽  
Nerve Growth

Changes in nerve growth factor content in superior cervical sympathetic ganglion after denervation or axotomy

1986 ◽  
Vol 3 ◽  
pp. S38
Author(s):  
Kazuya Takahama ◽  
Masato Ando ◽  
Mitsuyoshi Iwata ◽  
Shinichiro Hori ◽  
Kanefusa Kato ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sympathetic Ganglion ◽  
Factor Content ◽  
Nerve Growth ◽  
Cervical Sympathetic Ganglion ◽  
Superior Cervical Sympathetic Ganglion ◽  
Cervical Sympathetic

Immunology of nerve growth factor (NGF). The effect of NGF-antiserum on sensory ganglia in vitro

Development ◽  
1970 ◽  
Vol 23 (2) ◽  
pp. 273-287
Author(s):  
H. Hoffman
Keyword(s):  
Molecular Weight ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sympathetic Ganglion ◽  
Sympathetic Ganglia ◽  
Sensory Ganglia ◽  
Nerve Growth ◽  
Adult Organism

The properties of the ‘Nerve Growth Factor’ (NGF) have been described extensively (Levi-Montalcini & Booker, 1960; Levi-Montalcini, 1965) and reviewed recently (Levi-Montalcini, 1966). This factor is a protein of molecular weight about 130000 in its aggregated form (Varon, Nomura & Shooter, 1967, 1968) but may be active in lower molecular weight forms (Cohen, 1959, 1960; Banks et al. 1968). It is widely distributed in the adult organism (Bueker, Schenkein & Bane, 1960) and exerts a controlling influence on the differentiation of sensory and sympathetic ganglia in developing chick embryos. In newborn mammals its administration influences sympathetic ganglion growth only. A possible role in the adult nervous system is suggested by Scott, Gutmann & Horsky (1966), who showed that injected NGF will increase protein synthesis in regenerating sensory neurons in vivo. Active proteins in complex biological systems may be removed in a highly selective fashion by specific antibodies which thus provide a valuable means of studying their action.

Expression of nerve growth factor (NGF) receptors in the developing inner ear of chick and rat

Development ◽  
1991 ◽  
Vol 113 (2) ◽  
pp. 455-470 ◽  
Author(s):  
C.S. von Bartheld ◽  
S.L. Patterson ◽  
J.G. Heuer ◽  
E.F. Wheeler ◽  
M. Bothwell ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mrna Expression ◽  
Inner Ear ◽  
Ganglion Cells ◽  
Afferent Fiber ◽  
Secretory Cells ◽  
Nerve Growth ◽  
The Third ◽  
Pillar Cells

The expression of nerve growth factor receptors (NGFRs) was studied in the developing inner ear with in situ hybridization in chick embryos and with immunocytochemistry in rat embryos to determine sites of possible functions of NGF or NGF-like molecules in inner ear development. NGFR expression in the chick otocyst and acoustic ganglion is compared with epithelial differentiation and the onset of afferent innervation as determined with fluorescent carbocyanine tracers. In the inner ear of the chick embryo, NGFR mRNA expression shows an alternating pattern in mesenchymal and epithelial tissues. NGFR mRNA is heavily expressed in the mesenchyme surrounding the otocyst (E2-3), ceases at E3-5, and reappears in a thin layer of mesenchymal cells surrounding the membraneous epithelia (E5-13). In the otocyst epithelium, NGFR mRNA expression develops in one anterior and one posterior focus at E3-4.5. NGFR mRNA is expressed in the primordia of the ampullary cristae (E5-7) and possibly the anlage of the utricle; label transiently concentrates in the planum semilunatum of the cristae ampullares and in superior portions of the semicircular canals at E9, but is not seen in differentiating hair cells. In the acoustic ganglion, NGFR mRNA expression begins at E4; at the same time, the first peripheral acoustic nerve processes penetrate the otic epithelium (E4-4.5). The acoustic ganglia remain weakly NGFR mRNA-labeled in the posthatch animal. In the rat embryo, NGFR immunoreactivity is present in the auditory placode at E9, in the periotic mesenchyme at E9-10, and in the medial half of the otocyst at E10-11. At E12, epithelial NGFR expression becomes restricted anteriorly and posteriorly in a pattern similar to that of the chick otocyst and ceases at E13. NGFR immunoreactivity appears transiently in pillar cells of the cochlea in the third week of gestation. NGFR and NGFR mRNA is expressed after E11 in the acoustic ganglia. While NGFR transcripts are expressed in the cochlear ganglion cell bodies, NGFR protein becomes restricted to neuronal processes by the third week of gestation. The vestibular, but not the cochlear (spiral) ganglia remain NGFR-labeled in the adult rat. Onset of NGFR mRNA expression in the acoustic ganglion during the period of afferent fiber ingrowth into the otocyst epithelium is consistent with the hypothesis that NGF-like molecules may have a neurotrophic function for acoustic ganglion cells. Transient expression of NGFRs in secretory cells of the vestibular endorgan and pillar cells in the organ of Corti implicate a role for neurotrophins in the differentiation of these epithelial cell types.

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