Epidemiologic Typing of Methicillin-Resistant Staphylococcus aureus by Macrorestriction Analysis of Genomic DNA Using Two Different Restriction Enzymes

1998 ◽  
Vol 4 (2) ◽  
pp. 56-63
Author(s):  
Yoko Tabe ◽  
Katsu Saionji ◽  
Aayako Nakamura ◽  
Jun Igari
Keyword(s):  
Staphylococcus Aureus ◽  
Genomic Dna ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Restriction Enzymes ◽  
Methicillin Resistant

scholarly journals Impact of target site distribution for Type I restriction enzymes on the evolution of methicillin-resistant Staphylococcus aureus (MRSA) populations

2013 ◽  
Vol 41 (15) ◽  
pp. 7472-7484 ◽  
Author(s):  
Gareth A. Roberts ◽  
Patrick J. Houston ◽  
John H. White ◽  
Kai Chen ◽  
Augoustinos S. Stephanou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Restriction Enzymes ◽  
Target Site ◽  
Type I ◽  
Methicillin Resistant ◽  
Site Distribution

scholarly journals Methicillin-resistant Staphylococcus aureus from China characterized by digestion of total DNA with restriction enzymes

1989 ◽  
Vol 103 (1) ◽  
pp. 183-192 ◽  
Author(s):  
L. M. C. Hall ◽  
J. Z. Jordens ◽  
F. Wang
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Restriction Enzymes ◽  
Plasmid Profile ◽  
Single Strain ◽  
Methicillin Resistant ◽  
Additional Information ◽  
Antibiotic Resistance Profile ◽  
Total Dna ◽  
Cellular Dna

SUMMARYA series of clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) from two hospitals in China was examined. Fragment patterns obtained by digestion of total cellular DNA with restriction enzymes were used to characterize the isolates, in combination with phage-typing, antibiotic resistance profile, and plasmid profile. Digestion of total cellular DNA with restriction enzymes was most useful in discriminating between isolates and yielded additional information on the relatedness of non-identical isolates. In one hospital a single strain, resistant to a large number of antibiotics, had apparently become endemic. In the second hospital a number of distinct but related strains were present. The isolates were also related but not identical to the strain of MRSA endemic at the London Hospital.

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