Thermodesulforhabdus norvegicus gen. nov., sp. nov., a novel thermophilic sulfate-reducing bacterium from oil field water

1995 ◽  
Vol 164 (5) ◽  
pp. 331-336 ◽  
Author(s):  
Janiche Beeder ◽  
Terje Torsvik ◽  
Torleiv Lien
Keyword(s):  
Oil Field ◽  
Sulfate Reducing ◽  
Oil Field Water

Molecular- and cultivation-based analyses of microbial communities in oil field water and in microcosms amended with nitrate to control H2S production

2010 ◽  
Vol 89 (6) ◽  
pp. 2027-2038 ◽  
Author(s):  
Raji Kumaraswamy ◽  
Sara Ebert ◽  
Murray R. Gray ◽  
Phillip M. Fedorak ◽  
Julia M. Foght
Keyword(s):  
Microbial Communities ◽  
Oil Field ◽  
H2s Production ◽  
Oil Field Water

Characterization of the diversity of sulfate-reducing bacteria in soil and mining waste water environments by nucleic acid hybridization techniques

1994 ◽  
Vol 40 (11) ◽  
pp. 955-964 ◽  
Author(s):  
Anita J. Telang ◽  
Gerrit Voordouw ◽  
Sara Ebert ◽  
Neili Sifeldeen ◽  
Julia M. Foght ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Bacterial Communities ◽  
Genomic Dna ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Nucleic Acid Hybridization ◽  
Gene Probe ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Field Production

Nucleic acid hybridization techniques were used to characterize the sulfate-reducing bacterial communities at seven waste water and two soil sites in Canada. Genomic DNA was obtained from liquid enrichment cultures of samples taken from these nine sites. The liquid enrichment protocol favored growth of the sulfate-reducing bacterial component of the communities at these sites. The genomic DNA preparations were analyzed with (i) a specific gene probe aimed at a single genus (Desulfovibrio), (ii) a general 16S rRNA gene probe aimed at all genera of sulfate-reducing bacteria and other bacteria, and (iii) whole genome probes aimed at specific bacteria. This three-pronged approach provided information on the sulfate-reducing bacterial community structures for the nine sites. These were compared with each other and with the sulfate-reducing bacterial communities of western Canadian oil field production waters, studied previously. It was found that there is considerable diversity in the sulfate-reducing bacterial community at each site. Most sulfate-reducing bacteria isolated from distinct sites are genomically different and differ also from sulfate-reducing bacteria found in oil field production waters.Key words: sulfate-reducing bacteria, genomic diversity, nucleic acid hybridization, microbial community.

Applying a most probable number method for enumerating planktonic, dissimilatory, ammonium-producing, nitrate-reducing bacteria in oil field waters

2005 ◽  
Vol 51 (8) ◽  
pp. 725-729 ◽  
Author(s):  
Ruth E Eckford ◽  
Phillip M Fedorak
Keyword(s):  
Hydrogen Sulfide ◽  
Water Samples ◽  
Oil Field ◽  
Most Probable Number ◽  
Minor Role ◽  
Probable Number ◽  
A Minor ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria ◽  
Oil Field Water

A most probable number (MPN) method was used to enumerate dissimilatory ammonium-producing, nitrate-reducing bacteria (DAP-NRB) in oil field waters and to determine whether they were stimulated by nitrate addition used to control hydrogen sulfide production. An ammonium production medium with 5 carbon and energy sources (acetate, glucose, glycerol, pyruvate, and succinate) and nitrate was used in a 3-tube MPN procedure to enumerate DAP-NRB. These bacteria were detected in 12 of 18 oil field water samples, but they were seldom detected in wellhead samples. Three oil field water samples were amended with nitrate in serum bottles and the numbers of different NRB were determined over a 38-day incubation time. This amendment stimulated increases in the numbers of heterotrophic NRB and autotrophic nitrate-reducing, sulfide-oxidizing bacteria, but DAP-NRB remained a minor portion of these communities. Overall, DAP-NRB were present in many of the oil field waters that were examined but their numbers were low. It appears that DAP-NRB would play a minor role in the consumption of nitrate injected into oil field waters for the control of hydrogen sulfide production.Key words: heterotroph, nitrate-reducing bacteria, dissimilatory nitrate reduction, ammonium, petroleum.

The Study on the Inhibiting Effect of Denitrifying Bacteria on Sulfate-Reducing Process

2012 ◽  
Vol 260-261 ◽  
pp. 1210-1214
Author(s):  
Yan Li ◽  
Xuan Zhang ◽  
Li Hui Zhou ◽  
Jian Ke Ren ◽  
Nan Zhao
Keyword(s):  
Reduction Process ◽  
Denitrifying Bacteria ◽  
Average Concentration ◽  
Oil Field ◽  
Oil Fields ◽  
Uasb Reactor ◽  
Sulfate Reducing ◽  
Inhibiting Effect ◽  
Uasb Reactors ◽  
Limiting Value

The average concentration of H2S in Changqing oil Field is higher than 100mg/m3,which is much higher than the demand of “Operational Site Harm Factor Occupation Contact Limiting Value” (GBZ2-2002). Taking this factor into consideration, the experiment aimed at reducing H2S concentration in crude oil gatering and transferring systems by using the denitrifying bacteria. The experiment has two parts: First sulphat reduction process and then denitrification inhibit process. Therefore, two UASB reactors are used in a series to achieve these two processes. The first UASB reactor are mainly used to create SRB bacteria and H2S gas .After the first UASB reactor successfully starting, sodium nitrite are added in the second UASB to achieve denitrification inhibit process. The experiment showed that after the first UASB reactor start, the S2- can achieve 120mg/L and SO42- concentration is about 200 mg/L. So the Sulfate conversion rate can achieve 75%. Then, when the second UASB reactor started successfully, the S2- concentration sharply droped into 20 mg/L and SO42- concentration increased to 400mg/L, the sulphat reduction can achieve 83%. Also, advanced research showed that both SO42-concentration ratio and ORP value can contribute to restraining the SRB bacteria.Finally，through DGGE technology,many kinds of bacteria take into this process, for example pseudomonas aeruginosa. The results of this experiment has a great value to refrain the concentration of H2S in oil fields.

scholarly journals The Self Ignition of Fiberglass Lines

2014 ◽  
Vol 39 ◽  
pp. 1-9
Author(s):  
Moussa Bounoughaz ◽  
Noura Touabi
Keyword(s):  
Microbial Population ◽  
Bacterial Species ◽  
Methanogenic Bacteria ◽  
Oil Field ◽  
Microbiological Analysis ◽  
High Concentration ◽  
Sulfate Reducing ◽  
Maintenance Work ◽  
Reducing Bacteria

The fiberglass pipes were used for transportation of water in order to maintain the pressure in the oil field of Zarzaïtine (Region of In Amenas located in the south of Algeria). During the maintenance work in summer, a season well known for its extensive heat, the deposits contained in the pipe, and after its contact with the atmosphere, have caused a smoke and it was followed by a fire that ignited a portion of the pipe. To give an answer to the causes of this phenomenon, we have first incriminated the role of specific bacterial species and therefore we have made a microbiological analysis of the deposits collected from the site of the incident. The obtained results revealed the presence of a heterogeneous microbial population with a high concentration level of sulfate-reducing bacteria (SRB), methanogenic bacteria (MB), yeasts and fungi. The interaction between the different species of bacteria and the organic matter contained in the deposits has generated the formation of methane which under the influence of the great heat burnt and the fire caused the ignition of the fiberglass line.

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