Determination of lipid conjugated dienes with tetracyano-ethylene-14C: Significance for study of the pathology of lipid peroxidation

Lipids ◽  
1977 ◽  
Vol 12 (11) ◽  
pp. 914-921 ◽  
Author(s):  
Robert L. Waller ◽  
Richard O. Recknagel
Keyword(s):  
Lipid Peroxidation ◽  
Conjugated Dienes

scholarly journals Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease

2021 ◽  
Vol 709 ◽  
pp. 108941
Author(s):  
Cristina Mas-Bargues ◽  
Consuelo Escrivá ◽  
Mar Dromant ◽  
Consuelo Borrás ◽  
José Viña
Keyword(s):  
Lipid Peroxidation ◽  
Human Plasma ◽  
Reference Values ◽  
Chromatographic Determination ◽  
Health And Disease

Determination of conjugated dienes in gasoline by differential pulse polarography

1989 ◽  
Vol 61 (14) ◽  
pp. 1502-1504 ◽  
Author(s):  
Stephen J. Swarin ◽  
Kevin L. Perry
Keyword(s):  
Differential Pulse ◽  
Conjugated Dienes ◽  
Differential Pulse Polarography ◽  
Pulse Polarography

scholarly journals Elevated levels of erythrocyte-conjugated dienes indicate increased lipid peroxidation in schistosomiasis mansoni patients

2004 ◽  
Vol 37 (7) ◽  
pp. 957-962 ◽  
Author(s):  
H.T.F. Facundo ◽  
C.T. Brandt ◽  
J.S. Owen ◽  
V.L.M. Lima
Keyword(s):  
Lipid Peroxidation ◽  
Conjugated Dienes ◽  
Schistosomiasis Mansoni

Simultaneous measurements of conjugated dienes and free malondialdehyde, used as a micromethod for the evaluation of lipid peroxidation in rat hepatocyte cultures

1993 ◽  
Vol 65 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Odile Sergent ◽  
Isabelle Morel ◽  
Pascale Cogrel ◽  
Martine Chevanne ◽  
Nicole Pasdeloup ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Conjugated Dienes ◽  
Rat Hepatocyte ◽  
Hepatocyte Cultures ◽  
Simultaneous Measurements

scholarly journals Concentration of hepatic vitamins A and E in rats exposed to chlorpyrifos and/or enrofloxacin

2016 ◽  
Vol 19 (2) ◽  
pp. 371-378 ◽  
Author(s):  
A. Spodniewska ◽  
D. Barski
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Gastric Tube ◽  
Initial Period ◽  
Hplc Method ◽  
Antioxidant Vitamin ◽  
Exposure Group ◽  
Group Iii ◽  
Group I

Abstract The aim of the study was to determine the level of antioxidant vitamins A and E in the liver of rats exposed to chlorpyrifos and/or enrofloxacin. Chlorpyrifos (Group I) was administered at a dose of 0.04 LD50 (6 mg/kg b.w.) for 28 days, and enrofloxacin (Group II) at a dose of 5 mg/kg b.w. for 5 consecutive days. The animals of group III were given both of the mentioned above compounds at the same manner as groups I and II, but enrofloxacin was applied to rats for the last 5 days of chlorpyrifos exposure (i.e. on day 24, 25, 26, 27 and 28). Chlorpyrifos and enrofloxacin were administered to rats intragastrically via a gastric tube. The quantitative determination of vitamins was made by the HPLC method. The results of this study indicated a reduction in the hepatic concentrations of vitamins A and E, compared to the control, which sustained for the entire period of the experiment. The four-week administration of chlorpyrifos to rats resulted in a significant decrease of vitamins in the initial period of the experiment, i.e. up to 24 hours after exposure. For vitamin A the maximum drop was observed after 24 hours (19.24%) and for vitamin E after 6 hours (23.19%). Enrofloxacin caused a slight (3-9%) reduction in the level of the analysed vitamins. In the chlorpyrifos-enrofloxacin co-exposure group reduced vitamins A and E levels were also noted, but changes in this group were less pronounced in comparison to the animals intoxicated with chlorpyrifos only. The decrease in the antioxidant vitamin levels, particularly noticeable in the chlorpyrifos- and the chlorpyrifos combined with enrofloxacin-treated groups, may result not only from the increase in the concentration of free radicals, but also from the intensification of the secondary stages of lipid peroxidation.

Advances in methods for the determination of biologically relevant lipid peroxidation products

2010 ◽  
Vol 44 (10) ◽  
pp. 1172-1202 ◽  
Author(s):  
Corinne M. Spickett ◽  
Ingrid Wiswedel ◽  
Werner Siems ◽  
Kamelija Zarkovic ◽  
Neven Zarkovic
Keyword(s):  
Lipid Peroxidation ◽  
Biologically Relevant ◽  
Lipid Peroxidation Products

Determination of serum and organ malondialdehyde (MDA) concentration, a lipid peroxidation index, in Trypanosoma brucei-infected rats

2008 ◽  
Vol 17 (2) ◽  
pp. 67-72 ◽  
Author(s):  
J. I. Eze ◽  
B. M. Anene ◽  
C. C. Chukwu
Keyword(s):  
Lipid Peroxidation ◽  
Trypanosoma Brucei ◽  
Peroxidation Index

Lipid peroxidation and antielastase activity in the lung under oxidant stress: role of antioxidant defenses

1991 ◽  
Vol 70 (4) ◽  
pp. 1456-1462 ◽  
Author(s):  
V. Mohsenin
Keyword(s):  
Lipid Peroxidation ◽  
Oxidant Stress ◽  
Lavage Fluid ◽  
Conjugated Dienes ◽  
Antioxidant Defenses ◽  
Vitamin Supplementation ◽  
Control Group ◽  
Early Event ◽  
Lipid Peroxidation Products

The role of lipid peroxidation in the inactivation of alpha 1-protease inhibitor (alpha 1-PI) in the alveolar lining fluid of human subjects has been examined under oxidant stress. Exposure to nitrogen dioxide (NO2) at 4 ppm for 3 h resulted in a significant increase in the amount of lipid peroxidation products in the alveolar lining fluid, with conjugated dienes the predominant species. Four-week supplementation with vitamins C and E before NO2 exposure markedly decreased the levels of conjugated dienes (control 804 +/- 103 pmol/micrograms total phospholipids vs. vitamin-supplemented 369 +/- 58, P = 0.003). Malondialdehydes, although detectable in the lavage fluid, contributed little to the total amount of lipid peroxidation products, and the levels were comparable in both groups. NO2 exposure in the absence of vitamin supplementation caused a significant decrease in the elastase inhibitory capacity (EIC) of the alveolar lining fluid in the control group but not in the vitamin-supplemented group [control 3.67 +/- 0.32 micrograms alpha 1-PI/micrograms porcine pancreatic elastase (PPE) vs. vitamin-supplemented 2.75 +/- 0.17, P less than 0.03]. The vitamin-supplemented group had a lower level of conjugated dienes and a higher EIC. Conversely, the control group had higher levels of conjugated dienes and a lower EIC in their lavage fluid. These studies demonstrate that lipid peroxidation occurs as an early event during oxidant exposure in the lungs of normal subjects. The appearance of lipid peroxidation products in the lavage fluid is associated with a decrease in the EIC of the alveolar lining fluid. Vitamins C and E diminish lipid peroxidation and preserve the EIC of the lower respiratory tract fluid during oxidant stress.

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