Measurement of anisotropic vertebral trabecular bone loss during aging by quantitative image analysis

1988 ◽  
Vol 43 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Catherine Bergot ◽  
Anne-Marie Laval-Jeantet ◽  
Françoise Prêteux ◽  
Alain Meunier
2009 ◽  
Vol 42 (16) ◽  
pp. 2789-2796 ◽  
Author(s):  
P. Mc Donnell ◽  
N. Harrison ◽  
M.A.K. Liebschner ◽  
P.E. Mc Hugh

2009 ◽  
Vol 3 (5) ◽  
pp. 583-587 ◽  
Author(s):  
James R. Buchanan ◽  
Cathleen Myers ◽  
Tom Lloyd ◽  
Robert B. Greer

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 503-510 ◽  
Author(s):  
B. Burt-Pichat ◽  
M. H. Lafage-Proust ◽  
F. Duboeuf ◽  
N. Laroche ◽  
C. Itzstein ◽  
...  

Recent studies have demonstrated that bone is highly innervated and contains neuromediators that have functional receptors on bone cells. However, no data exist concerning the quantitative changes of innervation during bone loss associated with estrogen withdrawal. To study the involvement of nerve fibers in the regulation of bone remodeling, we have evaluated the modifications of innervation in a classical in vivo model of osteopenia in rats, ovariectomy (OVX). Skeletal innervation was studied by immunocytochemistry using antibodies directed against specific neuronal markers, neurofilament 200 and synaptophysin, and the neuromediator glutamate. Sciatic neurectomy, another model of bone loss due to limb denervation and paralysis, was used to validate our quantitative image analysis technique of immunostaining for nerve markers. Female Wistar rats at 12 wk of age were sham-operated (SHAM) or ovariectomized (OVX). Bone mineral density measurement and bone histomorphometry analysis of tibiae 14 d after surgery demonstrated a significant bone loss in OVX compared with SHAM. We observed an important reduction of nerve profile density in tibiae of OVX animals compared with SHAM animals, whereas innervation density in skin and muscles was similar for OVX and control rats. Quantitative image analysis of immunostainings demonstrated a significant decrease of the percentage of immunolabeling per total bone volume of neurofilament 200, synaptophysin, and glutamate in both the primary and secondary spongiosa of OVX rats compared with SHAM. These data indicate for the first time that OVX-induced bone loss in rat tibiae is associated with a reduction in nerve profile density, suggesting a functional link between the nervous system and the bone loss after ovariectomy.


Author(s):  
Vinod K. Berry ◽  
Xiao Zhang

In recent years it became apparent that we needed to improve productivity and efficiency in the Microscopy Laboratories in GE Plastics. It was realized that digital image acquisition, archiving, processing, analysis, and transmission over a network would be the best way to achieve this goal. Also, the capabilities of quantitative image analysis, image transmission etc. available with this approach would help us to increase our efficiency. Although the advantages of digital image acquisition, processing, archiving, etc. have been described and are being practiced in many SEM, laboratories, they have not been generally applied in microscopy laboratories (TEM, Optical, SEM and others) and impact on increased productivity has not been yet exploited as well.In order to attain our objective we have acquired a SEMICAPS imaging workstation for each of the GE Plastic sites in the United States. We have integrated the workstation with the microscopes and their peripherals as shown in Figure 1.


Author(s):  
Raimo Hartmann ◽  
Hannah Jeckel ◽  
Eric Jelli ◽  
Praveen K. Singh ◽  
Sanika Vaidya ◽  
...  

AbstractBiofilms are microbial communities that represent a highly abundant form of microbial life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space and time; microscopy and quantitative image analysis are therefore crucial for elucidating their functions. Here, we present BiofilmQ—a comprehensive image cytometry software tool for the automated and high-throughput quantification, analysis and visualization of numerous biofilm-internal and whole-biofilm properties in three-dimensional space and time.


2011 ◽  
Vol 55 (5) ◽  
pp. 455-459 ◽  
Author(s):  
Ryotaro Jingu ◽  
Masafumi Ohki ◽  
Sumiko Watanabe ◽  
Sadafumi Tamiya ◽  
Setsuo Sugishima ◽  
...  

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