Detection of bovine viruses in fetal bovine serum used in cell culture

In Vitro ◽  
1975 ◽  
Vol 11 (6) ◽  
pp. 400-403 ◽  
Author(s):  
A. J. Kniazeff ◽  
L. J. Wopschall ◽  
H. E. Hopps ◽  
C. S. Morris
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
Bovine Viruses

241 9.22 Performance of Gamma-irradiated Fetal Bovine Serum in Cell Culture

1994 ◽  
pp. 99-101
Author(s):  
J.D. Keathley ◽  
D. Wyatt ◽  
C.M. Williams ◽  
R. Festen ◽  
C. Maben
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
Gamma Irradiated

scholarly journals Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
In Vitro Cytotoxicity ◽  
Binding Energies ◽  
Docking Simulations ◽  
Fetal Bovine

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

scholarly journals Human Platelet Lysate as a Functional Substitute for Fetal Bovine Serum in the Culture of Human Adipose Derived Stromal/Stem Cells

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 724 ◽  
Author(s):  
Mathew Cowper ◽  
Trivia Frazier ◽  
Xiying Wu ◽  
J. Curley ◽  
Michelle Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Human Platelets ◽  
Dependent Manner ◽  
Wide Range ◽  
Human Ascs ◽  
Fetal Bovine ◽  
Stromal Stem Cells

Introduction: Adipose derived stromal/stem cells (ASCs) hold potential as cell therapeutics for a wide range of disease states; however, many expansion protocols rely on the use of fetal bovine serum (FBS) as a cell culture nutrient supplement. The current study explores the substitution of lysates from expired human platelets (HPLs) as an FBS substitute. Methods: Expired human platelets from an authorized blood center were lysed by freeze/thawing and used to examine human ASCs with respect to proliferation using hematocytometer cell counts, colony forming unit-fibroblast (CFU-F) frequency, surface immunophenotype by flow cytometry, and tri-lineage (adipocyte, chondrocyte, osteoblast) differentiation potential by histochemical staining. Results: The proliferation assays demonstrated that HPLs supported ASC proliferation in a concentration dependent manner, reaching levels that exceeded that observed in the presence of 10% FBS. The concentration of 0.75% HPLs was equivalent to 10% FBS when utilized in cell culture media with respect to proliferation, immunophenotype, and CFU-F frequency. When added to osteogenic, adipogenic, and chondrogenic differentiation media, both supplements showed appropriate differentiation by staining. Conclusion: HPLs is an effective substitute for FBS in the culture, expansion and differentiation of human ASCs suitable for pre-clinical studies; however, additional assays and analyses will be necessary to validate HPLs for clinical applications and regulatory approval.

Human Platelet Lysates Successfully Replace Fetal Bovine Serum in Adipose-Derived Adult Stem Cell Culture

2014 ◽  
Vol 2 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Gerhard Gstraunthaler ◽  
◽  
Caroline Rauch ◽  
Jaqueline Wechselberger ◽  
Elisabeth Feifel
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Adult Stem Cell ◽  
Stem Cell Culture ◽  
Fetal Bovine

Unbiased analysis by high throughput sequencing of the viral diversity in fetal bovine serum and trypsin used in cell culture

Biologicals ◽  
2014 ◽  
Vol 42 (3) ◽  
pp. 145-152 ◽  
Author(s):  
Léa Gagnieur ◽  
Justine Cheval ◽  
Marlène Gratigny ◽  
Charles Hébert ◽  
Erika Muth ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Bovine Serum ◽  
High Throughput Sequencing ◽  
Fetal Bovine Serum ◽  
Viral Diversity ◽  
Fetal Bovine

scholarly journals Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Liyuan Liu ◽  
Jinhuan Wang ◽  
Shengchang Duan ◽  
Lei Chen ◽  
Hui Xiang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Systematic Evaluation ◽  
Fetal Bovine

scholarly journals Human-Derived Alternatives to Fetal Bovine Serum in Cell Culture

2013 ◽  
Vol 40 (6) ◽  
pp. 417-423 ◽  
Author(s):  
Karin Witzeneder ◽  
Andrea Lindenmair ◽  
Christian Gabriel ◽  
Katharina Höller ◽  
Denise Theiß ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine
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