Microvascular endothelial cell cultures from human omental adipose tissue

1989 ◽  
Vol 25 (10) ◽  
pp. 863-864 ◽  
Author(s):  
Ann Knedler ◽  
Philip A. Kern ◽  
Robert H. Eckel ◽  
Richard G. Ham
Keyword(s):  
Adipose Tissue ◽  
Endothelial Cell ◽  
Cell Cultures ◽  
Microvascular Endothelial Cell ◽  
Omental Adipose Tissue ◽  
Endothelial Cell Cultures ◽  
Microvascular Endothelial ◽  
Human Omental Adipose Tissue

scholarly journals Human adipose tissue endothelial cells promote preadipocyte proliferation

2001 ◽  
Vol 281 (5) ◽  
pp. E1037-E1044 ◽  
Author(s):  
L. J. Hutley ◽  
A. C. Herington ◽  
W. Shurety ◽  
C. Cheung ◽  
D. A. Vesey ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Endothelial Cell ◽  
Human Adipose Tissue ◽  
Cell Types ◽  
Microvascular Endothelial Cell ◽  
Optimal Functioning ◽  
Energy Store ◽  
Endothelial Cell Cultures ◽  
Microvascular Endothelial

Adipogenesis is preceded by development of a microvascular network, and optimal functioning of adipose tissue as an energy store and endocrine organ is dependent on extensive vascularization. We have examined the role of endothelial cell-derived factors that influence the proliferation of human preadipocytes. Microvascular endothelial cells and preadipocytes were isolated from human omental and subcutaneous adipose tissue biopsies by use of a developed procedure of collagenase digest, immunoselection, and differential trypsinization. Conditioned medium from microvascular endothelial cell cultures promoted the proliferation of preadipocytes ( P = <0.001) and (to a lesser extent) other cell types. No depot-specific differences in mitogenic capacity of microvascular endothelial cell medium or of preadipocyte response were observed. These results indicate that adipose tissue endothelial cells secrete soluble adipogenic factor(s).

A Porcine Model for Adipose Tissue-Derived Endothelial Cell Transplantation

1992 ◽  
Vol 1 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Carlton Young ◽  
Bruce E. Jarrell ◽  
James B. Hoying ◽  
Stuart K. Williams
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Endothelial Cell ◽  
Cell Transplantation ◽  
Animal Studies ◽  
Porcine Model ◽  
Cell Isolation ◽  
Microvascular Endothelial Cell ◽  
Microvascular Endothelium ◽  
Microvascular Endothelial

The transplantation of endothelial cells represents a technology which has been suggested for applications ranging from improvement in function of implanted vascular devices to genetic therapy. The use of microvascular endothelial cell transplantation has seen increased use both in animal studies as well as clinical use. This report describes our techniques for the isolation and establishment of initial cultures of microvascular endothelial cells derived from porcine fat. A variety of anatomic sites within the pig were evaluated to determine the appropriateness of different sources of fat for endothelial cell isolation. The properitoneal fat was determined to be optimal due to the predominance of endothelium in this tissue and the ease of isolation of microvascular endothelium following collagenase digestion. The study of endothelial cell transplantation in the porcine model is now possible using the methods described for adipose tissue-derived micro vessel endothelial cell isolation.

scholarly journals Stem cells from human cardiac adipose tissue depots show different gene expression and functional capacities

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Carmen Lambert ◽  
Gemma Arderiu ◽  
Maria Teresa Bejar ◽  
Javier Crespo ◽  
Maribel Baldellou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Endothelial Cell ◽  
Epicardial Adipose Tissue ◽  
Microvascular Endothelial Cell ◽  
Ventricular Muscle ◽  
Cellular Analysis ◽  
Adipose Tissue Depots ◽  
Cardiac Ventricular Muscle ◽  
Microvascular Endothelial

Abstract Background The composition and function of the adipose tissue covering the heart are poorly known. In this study, we have investigated the epicardial adipose tissue (EAT) covering the cardiac ventricular muscle and the EAT covering the left anterior descending artery (LAD) on the human heart, to identify their resident stem cell functional activity. Methods EAT covering the cardiac ventricular muscle was isolated from the apex (avoiding areas irrigated by major vessels) of the heart (ventricular myocardium adipose tissue (VMAT)) and from the area covering the epicardial arterial sulcus of the LAD (PVAT) in human hearts excised during heart transplant surgery. Adipose stem cells (ASCs) from both adipose tissue depots were immediately isolated and phenotypically characterized by flow cytometry. The different behavior of these ASCs and their released secretome microvesicles (MVs) were investigated by molecular and cellular analysis. Results ASCs from both VMAT (mASCs) and the PVAT (pASCs) were characterized by the expression of CD105, CD44, CD29, CD90, and CD73. The angiogenic-related genes VEGFA, COL18A1, and TF, as well as the miRNA126-3p and miRNA145-5p, were analyzed in both ASC types. Both ASCs were functionally able to form tube-like structures in three-dimensional basement membrane substrates. Interestingly, pASCs showed a higher level of expression of VEGFA and reduced level of COL18A1 than mASCs. Furthermore, MVs released by mASCs significantly induced human microvascular endothelial cell migration. Conclusion Our study indicates for the first time that the resident ASCs in human epicardial adipose tissue display a depot-specific angiogenic function. Additionally, we have demonstrated that resident stem cells are able to regulate microvascular endothelial cell function by the release of MVs.

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