Production of ethanol by cultured insect cells

Background: Baculoviruses are insect pathogens with important biotechnological applications that transcend their use as biological controllers of agricultural pests. One species, Autographa californica multiple nucleopolhyedrovirus (AcMNPV) has been extensively exploited as a molecular platform to produce recombinant proteins and as a delivery vector for genes in mammals, because it can transduce a wide range of mammalian cells and tissues without replicating or producing progeny. Objective/Method: To investigate if the budded virions of Anticarsia gemmatalis multiple nucleopolhyedrovirus (AgMNPV) species has the same ability, the viral genome was modified by homologous recombination into susceptible insect cells to integrate reporter genes and then it was evaluated on mammalian cell lines in comparative form with respect to equivalent viruses derived from AcMNPV. Besides, the replicative capacity of AgMNPV´s virions in mammals was determined. Results: The experiments carried out showed that the recombinant variant of AgMNPV transduces and support the expression of delivered genes but not replicates in mammalian cells. Conclusion: Consequently, this insect pathogen is proposed as an alternative of non-infectious viruses in humans to explore new approaches in gene therapy and other applications based on the use of mammalian cells.

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Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector.

Journal of Virology ◽

10.1128/jvi.64.10.4799-4807.1990 ◽

1990 ◽

Vol 64 (10) ◽

pp. 4799-4807 ◽

Cited By ~ 45

Author(s):

A Höss ◽

I Moarefi ◽

K H Scheidtmann ◽

L J Cisek ◽

J L Corden ◽

...

Keyword(s):

Insect Cells ◽

Simian Virus 40 ◽

Simian Virus ◽

T Antigen ◽

Baculovirus Vector ◽

Phosphorylation Pattern

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Comparative Analysis of the Roles of Simian Immunodeficiency and Bovine Leukemia Virus Matrix Proteins in Gag Assembly in Insect Cells

Virology ◽

10.1006/viro.2002.1441 ◽

2002 ◽

Vol 299 (1) ◽

pp. 48-55 ◽

Cited By ~ 2

Author(s):

Naresh K. Kakker ◽

Michael V. Mikhailov ◽

Ian M. Jones ◽

Polly Roy

Keyword(s):

Comparative Analysis ◽

Bovine Leukemia Virus ◽

Insect Cells ◽

Leukemia Virus ◽

Matrix Proteins ◽

Gag Assembly

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A Comparative Perspective on Functionally-Related, Intracellular Calcium Channels: The Insect Ryanodine and Inositol 1,4,5-trisphosphate Receptors

Biomolecules ◽

10.3390/biom11071031 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1031

Author(s):

Umut Toprak ◽

Cansu Doğan ◽

Dwayne Hegedus

Keyword(s):

Pest Control ◽

Structural Organization ◽

Insect Cells ◽

Expression Patterns ◽

Regulatory Mechanisms ◽

Comparative Approach ◽

Insect Development ◽

Current Review ◽

Inositol 1,4,5 Trisphosphate ◽

Target Sites

Calcium (Ca2+) homeostasis is vital for insect development and metabolism, and the endoplasmic reticulum (ER) is a major intracellular reservoir for Ca2+. The inositol 1,4,5- triphosphate receptor (IP3R) and ryanodine receptor (RyR) are large homotetrameric channels associated with the ER and serve as two major actors in ER-derived Ca2+ supply. Most of the knowledge on these receptors derives from mammalian systems that possess three genes for each receptor. These studies have inspired work on synonymous receptors in insects, which encode a single IP3R and RyR. In the current review, we focus on a fundamental, common question: “why do insect cells possess two Ca2+ channel receptors in the ER?”. Through a comparative approach, this review covers the discovery of RyRs and IP3Rs, examines their structures/functions, the pathways that they interact with, and their potential as target sites in pest control. Although insects RyRs and IP3Rs share structural similarities, they are phylogenetically distinct, have their own structural organization, regulatory mechanisms, and expression patterns, which explains their functional distinction. Nevertheless, both have great potential as target sites in pest control, with RyRs currently being targeted by commercial insecticide, the diamides.

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Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and inEscherichia coli

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.62.136 ◽

1998 ◽

Vol 62 (1) ◽

pp. 136-141 ◽

Cited By ~ 16

Author(s):

Kiyotaka HITOMI ◽

Akiko YOKOYAMA ◽

Masatoshi MAKI

Keyword(s):

Insect Cells ◽

Biologically Active ◽

Inescherichia Coli ◽

Infected Insect

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Bacmid Expression of Granulovirus Enhancin En3 Accumulates in Cell Soluble Fraction to Potentiate Nucleopolyhedrovirus Infection

Viruses ◽

10.3390/v13071233 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1233

Author(s):

Adriana Ricarte-Bermejo ◽

Oihane Simón ◽

Ana Beatriz Fernández ◽

Trevor Williams ◽

Primitivo Caballero

Keyword(s):

Trichoplusia Ni ◽

Recombinant Virus ◽

Spodoptera Exigua ◽

Insect Cells ◽

Soluble Fraction ◽

Autographa Californica ◽

Insect Midgut ◽

Baculovirus Infection ◽

Occlusion Bodies ◽

Infected Cells

Enhancins are metalloproteinases that facilitate baculovirus infection in the insect midgut. They are more prevalent in granuloviruses (GVs), constituting up to 5% of the proteins of viral occlusion bodies (OBs). In nucleopolyhedroviruses (NPVs), in contrast, they are present in the envelope of the occlusion-derived virions (ODV). In the present study, we constructed a recombinant Autographa californica NPV (AcMNPV) that expressed the Trichoplusia ni GV (TnGV) enhancin 3 (En3), with the aim of increasing the presence of enhancin in the OBs or ODVs. En3 was successfully produced but did not localize to the OBs or the ODVs and accumulated in the soluble fraction of infected cells. As a result, increased OB pathogenicity was observed when OBs were administered in mixtures with the soluble fraction of infected cells. The mixture of OBs and the soluble fraction of Sf9 cells infected with BacPhEn3 recombinant virus was ~3- and ~4.7-fold more pathogenic than BacPh control OBs in the second and fourth instars of Spodoptera exigua, respectively. In contrast, when purified, recombinant BacPhEn3 OBs were as pathogenic as control BacPh OBs. The expression of En3 in the soluble fraction of insect cells may find applications in the development of virus-based insecticides with increased efficacy.

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