Immunomagnetic separation as a final purification step of liver endothelial cells

1993 ◽  
Vol 29 (6) ◽  
pp. 451-455 ◽  
Author(s):  
Daniel E. Gomez ◽  
Jacqueline L. Hartzler ◽  
Robert H. Corbitt ◽  
Alexander M. Nason ◽  
Unnur P. Thorgeirsson
Keyword(s):  
Endothelial Cells ◽  
Immunomagnetic Separation ◽  
Purification Step ◽  
Final Purification Step ◽  
Liver Endothelial Cells ◽  
Final Purification

scholarly journals Amino Acid Sequence Studies on Sheep Liver Fructose-Bisphosphatase. I. The S-Peptide

1980 ◽  
Vol 33 (6) ◽  
pp. 665 ◽  
Author(s):  
WK Fisher ◽  
EOP Thompson
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Gel Electrophoresis ◽  
Fructose Bisphosphatase ◽  
Purification Step ◽  
Sheep Liver ◽  
Final Purification Step ◽  
Elution Chromatography ◽  
Final Purification

Fructose-bisphosphatase has been isolated from sheep liver using affinity-elution chromatography from carboxymethykellulose as the final purification step. The purified enzyme was homogeneous by disc gel electrophoresis.

Rat liver endothelial cells isolated by anti-CD31 immunomagnetic separation lack fenestrae and sieve plates

2006 ◽  
Vol 291 (6) ◽  
pp. G1187-G1189 ◽  
Author(s):  
Laurie D. DeLeve ◽  
Xiangdong Wang ◽  
Margaret K. McCuskey ◽  
Robert S. McCuskey
Keyword(s):  
Endothelial Cells ◽  
Gold Standard ◽  
Gradient Centrifugation ◽  
Immunomagnetic Separation ◽  
Centrifugal Elutriation ◽  
Liver Endothelial Cells ◽  
Sieve Plates ◽  
Immunomagnetic Sorting ◽  
Scanning Electron

The gold standard for the identification of sinusoidal endothelial cells (SEC) is the presence of fenestrae organized in sieve plates, which is characteristic of SEC in vivo. One of the methods currently in use to isolate SEC is immunomagnetic sorting for CD31. However, there is evidence to suggest that CD31 is not present on the surface of differentiated SEC. The present study used scanning electron microscopy to image rat hepatic endothelial cells isolated by anti-CD31 and immunomagnetic sorting and cells isolated by gradient centrifugation and centrifugal elutriation. Cells isolated by elutriation had well-developed fenestrae and sieve plates, whereas cells isolated by anti-CD31 and immunomagnetic sorting had significantly fewer fenestrae organized in sieve plates. In conclusion, cells isolated by anti-CD31 and immunomagnetic sorting lacked the hallmark features of SEC.

Optimization purity of isolated primary liver endothelial cells (LSEC) using adenoviral gene transfer

2014 ◽  
Vol 52 (01) ◽  
Author(s):  
V Sterzer ◽  
M Alsamman ◽  
R Weiskirchen ◽  
C Trautwein ◽  
D Scholten
Keyword(s):  
Endothelial Cells ◽  
Gene Transfer ◽  
Adenoviral Gene Transfer ◽  
Liver Endothelial Cells

Identification of Liver Endothelial Cells as the Primary Site of IgM Catabolism in the Rat

1995 ◽  
Vol 319 (1) ◽  
pp. 63-73 ◽  
Author(s):  
Z.C. Chroneos ◽  
J.W. Baynes ◽  
S.R. Thorpe
Keyword(s):  
Endothelial Cells ◽  
Primary Site ◽  
Liver Endothelial Cells

scholarly journals Chronic ethanol consumption impairs receptor-mediated endocytosis of MAA-modified albumin by liver endothelial cells

2003 ◽  
Vol 66 (6) ◽  
pp. 1045-1054 ◽  
Author(s):  
Michael J Duryee ◽  
Lynell W Klassen ◽  
Thomas L Freeman ◽  
Monte S Willis ◽  
Dean J Tuma ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ethanol Consumption ◽  
Chronic Ethanol ◽  
Receptor Mediated Endocytosis ◽  
Chronic Ethanol Consumption ◽  
Liver Endothelial Cells

794 TLR1/2 LIGAND STIMULATED MOUSE LIVER ENDOTHELIAL CELLS PRODUCE IL-12 AND TRIGGER CD8+ T-CELL IMMUNITY

2012 ◽  
Vol 56 ◽  
pp. S311
Author(s):  
J. Liu ◽  
M. Jiang ◽  
Z. Ma ◽  
J. Schlaak ◽  
M. Roggendorf ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
T Cell ◽  
Mouse Liver ◽  
Cd8 T Cell ◽  
T Cell Immunity ◽  
Cell Immunity ◽  
Liver Endothelial Cells

Endothelin-1 production by hepatic endothelial cells: characterization and augmentation by endotoxin exposure

1997 ◽  
Vol 272 (3) ◽  
pp. G605-G611 ◽  
Author(s):  
A. T. Eakes ◽  
K. M. Howard ◽  
J. E. Miller ◽  
M. S. Olson
Keyword(s):  
Endothelial Cells ◽  
Significant Contribution ◽  
Gradual Increase ◽  
Glucose Production ◽  
Cell Types ◽  
Exposure Model ◽  
Mrna Levels ◽  
Endotoxin Challenge ◽  
Endotoxin Exposure ◽  
Liver Endothelial Cells

Activation of endothelin (ET) receptors in the liver causes vasoconstriction, glucose production, and lipid and peptide mediator synthesis. In the intact rat, a bolus infusion of endotoxin into a mesenteric vein served as an acute exposure model of endotoxemia. In response to this challenge, a ninefold increase in hepatic ET-1 mRNA occurred within 3 h. The plasma level of immunoreactive ET-1 (irET-1) increased correspondingly by 8.5-fold within 6 h. ET-1 mRNA levels in liver endothelial cells (EC) isolated from livers of endotoxin-treated rats at various times after endotoxin challenge showed a more gradual increase. Northern blot analyses of the major liver cell types demonstrated that ET-1 mRNA was most abundant in the EC. The present results document a significant increase in the circulating level of irET-1 during episodes of endotoxemia. The increased hepatic ET-1 production in response to endotoxin infusion suggests that ET-1 produced in the liver could make a significant contribution to the plasma irET-1 and may be an important component in the hepatic responses to systemic trauma.

Morphological studies on endocytosis of chondroitin sulphate proteoglycan by rat liver endothelial cells

1988 ◽  
Vol 253 (1) ◽  
Author(s):  
B�rd Smedsr�d ◽  
Marie Malmgren ◽  
Jan Ericsson ◽  
TorvardC. Laurent
Keyword(s):  
Endothelial Cells ◽  
Rat Liver ◽  
Chondroitin Sulphate ◽  
Sulphate Proteoglycan ◽  
Morphological Studies ◽  
Chondroitin Sulphate Proteoglycan ◽  
Liver Endothelial Cells
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