Primary culture of antvenom gland cells

In Vitro ◽  
1985 ◽  
Vol 21 (6) ◽  
pp. 333-339 ◽  
Author(s):  
W. F. Hink ◽  
J. J. Butz
Keyword(s):  
Primary Culture ◽  
Gland Cells

Control of acid secretion in cultured gas gland cells of the European eel Anguilla anguilla

1996 ◽  
Vol 270 (3) ◽  
pp. R578-R584
Author(s):  
B. Pelster ◽  
L. Pott
Keyword(s):  
Acid Secretion ◽  
Primary Culture ◽  
Single Cells ◽  
Calf Serum ◽  
Anguilla Anguilla ◽  
Beta Agonist ◽  
European Eel ◽  
Adrenergic Stimulation ◽  
Gland Cells ◽  
Gas Gland

Single cells and cell clusters isolated from the swimbladder epithelium of the European eel Anguilla anguilla attached to collagen S-coated petri dishes and proliferated in a modified Dulbecco's modified Eagle's medium, supplemented with 0.5% fetal calf serum. At a temperature of 20-22 degrees C, the growing colonies reached confluence typically within 6-8 days. Activities of glycolytic and pentose phosphate shunt enzymes remained stable or increased only slightly during the first 10 days of primary culture. Incubated in a defined medium providing glucose as a fuel, gas gland cells in primary culture produced and released lactic acid. The rate of acid secretion of cultured gas gland cells measured with a cytosensor microphysiometer was not influenced by cholinergic stimulation. Similarly, the Ca2+ ionophore A-23187 had no effect. Adrenergic stimulation with epinephrine or the beta-agonist isoproterenol also did not increase the rate of acid secretion, indicating that in gas gland cells the metabolic activity cannot be stimulated via beta-adrenergic stimulation followed by an increase in adenosine 3',5'-cyclic monophosphate (cAMP). Artificially increasing the intracellular concentration of cAMP by incubation with forskolin or the cAMP analogue 8-(4-chlorophenylthio)-cAMP even resulted in a marked reduction in the rate of acid secretion. The results demonstrate that primary cell culture provides a useful means for the analysis of metabolic control and of ion transfer processes in swimbladder gas gland cells.

scholarly journals The effects of isoproterenol on the growth of human salivary gland cells in primary culture.

1987 ◽  
Vol 33 (12) ◽  
pp. 2358-2365
Author(s):  
Kazuyuki MIYATA ◽  
Koshin IWAGAMI ◽  
Takeshi WADA ◽  
Masakazu HARADA ◽  
Kenji TAKAGI ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Primary Culture ◽  
Human Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells

Primary culture of venom gland cells from the South American rattlesnake (Crotalus durissus terrificus)

Toxicon ◽  
1999 ◽  
Vol 37 (12) ◽  
pp. 1673-1682 ◽  
Author(s):  
M.M. Duarte ◽  
H. Montes De Oca ◽  
C.R. Diniz ◽  
C.L. Fortes-Dias
Keyword(s):  
Primary Culture ◽  
Venom Gland ◽  
South American ◽  
The South ◽  
Crotalus Durissus Terrificus ◽  
Gland Cells ◽  
Crotalus Durissus

scholarly journals Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells

2010 ◽  
Vol 105 (2) ◽  
pp. 132-136 ◽  
Author(s):  
Fernanda F Rocha ◽  
Ricardo N Araujo ◽  
Luciana M Silva ◽  
Nelder F Gontijo ◽  
Marcos H Pereira
Keyword(s):  
Salivary Gland ◽  
Primary Culture ◽  
Rhodnius Prolixus ◽  
Gland Cells ◽  
Salivary Gland Cells

Cat tracheal gland cells in primary culture

1992 ◽  
Vol 263 (2) ◽  
pp. L264-L275 ◽  
Author(s):  
D. J. Culp ◽  
D. K. Lee ◽  
D. P. Penney ◽  
M. G. Marin
Keyword(s):  
Molecular Weight ◽  
Primary Culture ◽  
High Molecular Weight ◽  
Cultured Cells ◽  
Short Circuit ◽  
Light And Electron Microscopy ◽  
Collagen Gels ◽  
Culture Dish ◽  
Gland Cells ◽  
Secretion Granules

Conditions for the primary culture of isolated cat tracheal gland cells were established. Cells plated onto glutaraldehyde-fixed gels of rat tail collagen grew to confluency after 8 days of culture forming a monolayer of cuboidal cells with ultrastructural characteristics of epithelial cells and immunoreactivity to antikeratins. Cultured cells synthesized and released radiolabeled high-molecular-weight glycoconjugates. Glycoconjugate secretion was increased approximately 10% in response to the cholinergic agonist, carbachol. Secretion of glycoconjugates was unrelated to regulated exocrine secretion, since these cells were devoid of secretion granules as assessed by light and electron microscopy. Confluent cultures also generated a spontaneous potential difference and short-circuit current, which were both inhibited by ouabain and increased by carbachol. This suggested gland cells contribute to fluid secretion by active ion-transport mechanisms. We also plated cells onto unfixed collagen gels that were released from the culture dish at confluency. Cells were columnar with apically oriented secretion granules that stained with alcian blue and for blood group A immunoreactivity. Secretion of radiolabeled high-molecular-weight glycoconjugates was increased 27% by carbachol. These cell culture systems may serve as models to investigate glandular secretory mechanisms and their regulation.

scholarly journals Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis

2004 ◽  
Vol 25 (3-4) ◽  
pp. 177-184 ◽  
Author(s):  
J�r�me Faucet ◽  
Manuelle Maurice ◽  
B�atrice Gagnaire ◽  
Tristan Renault ◽  
Thierry Burgeot
Keyword(s):  
Primary Culture ◽  
Mytilus Edulis ◽  
Digestive Gland ◽  
Gland Cells ◽  
The Common

Primary culture of rat ependymal cells in serum-free defined medium

1986 ◽  
Vol 390 (2) ◽  
pp. 199-209 ◽  
Author(s):  
M WEIBEL ◽  
B PETTMANN ◽  
J ARTAULT ◽  
M SENSENBRENNER ◽  
G LABOURDETTE
Keyword(s):  
Primary Culture ◽  
Defined Medium ◽  
Ependymal Cells ◽  
Serum Free
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