Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment

The implantation of the embryo into the uterus requires hatching from its zona pellucida (ZP). The inability of the embryo to break its zona pellucida is considered as a factor for implantation failure. Assisted hatching (AH) is performed to make it easier for natural hatching to occur, also providing early embryoendometrium contact, which favors the embryos implantation into the uterus. To evaluate the effect of laser assisted hatching (LAH) on pregnancy rate in frozen embryo transfer cycle.  Materials & methods: In a prospective observational study a total of 80 patients who underwent frozen embryo transfer(FET) cycles were included in the study. Patients were divided into Laser assisted hatching group (LAH) and no LAH group. In the LAH group, zona thinning was done with the help of laser just prior to the embryo transfer. In the control group no hatching was done before transfer. The main outcome measures were pregnancy rate & implantation rate. The baseline characteristics of the 80 patients included in the study ie.,LAH group (n=40) vs no LAH group (n=40),the mean age ( 30.855.4 vs 333.9),mean BMI (25.64.1 vs 27.94.5),duration of infertility ( 6.184.1 vs 8.534.7) , number of embryos transferred (2.750.8 vs 2.70.72) respectively. Younger women < 30 years in no LAH group had higher pregnancy rate compared to the LAH group. Between the two groups, patients between 31-35 years in LAH group had higher pregnancy rate compared to no LAH group. The pregnancy rate (42.85% vs 54.5%) in LAH group of women >35years found lower than the no LAH group. LAH seems to be beneficial in women between 31- 35 years of age group, but LAH does not seem to be beneficial in women > 35 years of age. There is slight increase in pregnancy rates with laser assisted zona thinning in frozen transfer cycle, but it is not statistically significant.

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USE OF CIDR AND ESTRADIOL CYPIONATE IN SYNCHRONIZATION PROTOCOLS ON ESTRUS PRESENTATION, PREGNANCY AND BIRTH RATE IN CREOLE SHEEP UNDER HIGH ALTITUDE CONDITIONS

SPERMOVA ◽

10.18548/aspe/0009.15 ◽

2021 ◽

Vol 11 (2) ◽

pp. 109-114

Author(s):

Nubia Catacora ◽

◽

Wilber García ◽

Uri Perez ◽

Eloy Condori ◽

...

Keyword(s):

High Altitude ◽

Pregnancy Rate ◽

Birth Rate ◽

Chi Square ◽

High Pregnancy Rate ◽

Chi Square Test ◽

The Difference ◽

Intravaginal Device ◽

Fresh Semen ◽

Pregnancy And Birth

The objective of this research was to evaluate the use of the intravaginal device (CIDR) and estradiol cypionate in synchronization protocols, on oestrus presentation, pregnancy and birth rate in Creole sheep under high altitude conditions. The study was carried out in a non-reproductive season, in the peruvian highlands, using 57 ewes. Four experimental groups were formed: group T1 (n=14) and T3 (n=14), CIDR progesterone device for 7 days and 12 days, respectively; group T2 (n=15) and T4 (n=14), CIDR progesterone device for 7 days and 12 days plus 1 mg of estradiol cypionate, 24 hours after removal of the device in both groups, respectively. Estrous presentation was observed from CIDR removal to 48 hours using vasectomized rams and IATF was performed with fresh semen 48 hours after device removal. The pregnancy rate was determined by ultrasonography at 46 and 90 days after FTAI and the birth rate was recorded. The chi-square test was used for statistical analysis. There was no difference (P>0.05) between groups, for estrous presentation, but there was difference (P<0.05) for pregnancy rate at 46 days between groups with: T1 (42.9%), T3 (38.5%), T4 (21.4%), with respect to T2 (0%) (The difference in results are shown in Table 2). There was a difference (P<0.05) for the pregnancy rate at 90 days: T1 (35.7%) and T3 (30.8%), with respect to T4 (7.1%) and T2 (0 %). The birth rate showed differences (P<0.05) for T1 (28.6%) and T3 (28.6%), with respect to T2 (0%) and T4 (0%). In conclusion, a high pregnancy rate was obtained with CIDR for 7 and 12 days, compared to the use of CIDR plus estradiol cypionate. However, no births were obtained with progesterone plus estradiol cypionate treatment.

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