scholarly journals Effects of Laser Assisted Hatching on Pregnancy Outcomes of Frozen Embryo Transfer Cycles

2020 ◽  
Vol 5 (9) ◽  
pp. 1223-1223
Author(s):  
Dr. Narmadha. R ◽  
Dr . Manjula ◽  
Dr. N.Sanjeeva Reddy ◽  
Dr. Sindhuja. N.S
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Zona Pellucida ◽  
Implantation Rate ◽  
Frozen Embryo Transfer ◽  
Control Group ◽  
Assisted Hatching ◽  
Younger Women ◽  
The Mean ◽  
Baseline Characteristics

The implantation of the embryo into the uterus requires hatching from its zona pellucida (ZP). The inability of the embryo to break its zona pellucida is considered as a factor for implantation failure. Assisted hatching (AH) is performed to make it easier for natural hatching to occur, also providing early embryoendometrium contact, which favors the embryos implantation into the uterus. To evaluate the effect of laser assisted hatching (LAH) on pregnancy rate in frozen embryo transfer cycle.  Materials & methods: In a prospective observational study a total of 80 patients who underwent frozen embryo transfer(FET) cycles were included in the study. Patients were divided into Laser assisted hatching group (LAH) and no LAH group. In the LAH group, zona thinning was done with the help of laser just prior to the embryo transfer. In the control group no hatching was done before transfer. The main outcome measures were pregnancy rate & implantation rate. The baseline characteristics of the 80 patients included in the study ie.,LAH group (n=40) vs no LAH group (n=40),the mean age ( 30.855.4 vs 333.9),mean BMI (25.64.1 vs 27.94.5),duration of infertility ( 6.184.1 vs 8.534.7) , number of embryos transferred (2.750.8 vs 2.70.72) respectively. Younger women < 30 years in no LAH group had higher pregnancy rate compared to the LAH group. Between the two groups, patients between 31-35 years in LAH group had higher pregnancy rate compared to no LAH group. The pregnancy rate (42.85% vs 54.5%) in LAH group of women >35years found lower than the no LAH group. LAH seems to be beneficial in women between 31- 35 years of age group, but LAH does not seem to be beneficial in women > 35 years of age. There is slight increase in pregnancy rates with laser assisted zona thinning in frozen transfer cycle, but it is not statistically significant.

A Randomized Trial of Partial Versus Complete Versus Not Using Laser-Assisted Hatching in Vitrified-Warmed Cleavage Embryo Transfer: A Preliminary Report

2021 ◽  
Vol 104 (1) ◽  
pp. 18-23
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Live Birth ◽  
Birth Rate ◽  
Implantation Rate ◽  
Live Birth Rate ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Assisted Hatching ◽  
Cleavage Stage

Background: Currently, the effect of laser-assisted hatching (LAH) on the outcome of cryopreserved embryo remains controversial and unclear, especially on the cryopreserved embryos using a novel vitrification method. Objective: To compare the pregnancy outcomes of vitrified-warmed cleavage stage embryos transfer using LAH breaching or LAH thinning versus those not using LAH. Materials and Methods: Sixty patients with vitrified-warmed cleavage embryo transfer were randomly assigned to a control group without LAH treatment, LAH-breeching group, and LAH-thinning group. The outcome measurements were clinical pregnancy rate, implantation rate, and live birth rate. Results: The clinical pregnancy rate (35% versus 20% versus 25%) and implantation rate (17.3% versus 11.5% versus 11.3%) were lower in both LAH-breaching and LAH-thinning group than the control group, but not statistically significant (p>0.05). The live birth rate (30% versus 5% versus 5%) was significantly lower in both the LAH-breaching and LAH-thinning group than the control group (p=0.026). Conclusion: LAH regardless of breaching or thinning methods significantly decreases live birth rate in vitrified-warmed cleavage-stage embryo transfer. Keywords: Laser-assisted hatching, Vitrified-warmed, Cleavage embryo

scholarly journals Should rescue ICSI be re-evaluated considering the deferred transfer of cryopreserved embryos in in-vitro fertilization cycles? A systematic review and meta-analysis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Alessio Paffoni ◽  
Marco Reschini ◽  
Valerio Pisaturo ◽  
Cristina Guarneri ◽  
Simone Palini ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Implantation Rate ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Rescue Icsi

Abstract Background Total fertilization failure represents a particularly frustrating condition for couples undergoing in vitro fertilization. With the aim of reducing the occurrence of total fertilization failure, intracytoplasmic sperm injection (ICSI) has become the first choice over conventional in vitro fertilization (IVF) procedures although evidence of improved results is still debated and its use in couples without male factor infertility is not recommended. Among the strategies potentially useful to promote the use of conventional IVF, we herein call attention to the late rescue ICSI, which consists in performing ICSI after 18–24 h from conventional insemination on oocytes that show no signs of fertilization. This treatment has however been reported to be associated with a low success rate until recent observations that embryos derived from late rescue ICSI may be transferred after cryopreservation in a frozen-thawed cycle with improved results. The aim of the present study was to assess whether frozen embryos deriving from rescue ICSI performed about 24 h after conventional IVF may represent a valuable option for couples experiencing fertilization failure. Methods A systematic review on the efficacy of late rescue ICSI was performed consulting PUBMED and EMBASE. Results Including twenty-two original studies, we showed that clinical pregnancy rate per embryo transfer and implantation rate obtainable with fresh embryo transfers after rescue ICSI are not satisfactory being equal to 10 and 5%, respectively. The transfer of cryopreserved rescue ICSI embryos seems to offer a substantial improvement of success rates, with pregnancy rate per embryo transfer and implantation rate equal to 36 and 18%, respectively. Coupling rescue ICSI with frozen embryo transfer may ameliorate the clinical pregnancy rate for embryo transfer with an Odds Ratio = 4.7 (95% CI:2.6–8.6). Conclusion Results of the present review support the idea that r-ICSI coupled with frozen embryo transfer may overcome most of the technical and biological issues associated with fresh transfer after late r-ICSI, thus possibly representing an efficient procedure for couples experiencing fertilization failure following conventional IVF cycles. Trial registration Prospero registration ID: CRD42021239026.

Combined treatment of prednisone and hydroxychloroquine may improve outcomes of frozen embryo transfer in antinuclear antibody-positive patients undergoing IVF/ICSI treatment

Lupus ◽  
2021 ◽  
pp. 096120332110558
Author(s):  
Rui Gao ◽  
Wei Deng ◽  
Cheng Meng ◽  
Kemin Cheng ◽  
Xun Zeng ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Pregnancy Loss ◽  
Loss Rate ◽  
Implantation Rate ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Clinical Pregnancy Rate ◽  
High Quality ◽  
Biochemical Pregnancy

Background The influence of anti-nuclear antibody (ANA) on induced ovulation was controversial, and the effect of prednisone plus hydroxychloroquine (HCQ) treatment on frozen embryo transfer outcomes of in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) for ANA-positive women was unclear. Methods Fifty ANA-positive women and one-hundred ANA-negative women matched for age and anti-Mullerian hormone (AMH) were included from a Reproductive Medical Central of a University Hospital. Sixty-one oocytes pick-up (OPU) cycles in ANA+ group and one-hundred OPU cycles in ANA− group were compared; 30 frozen embryo transfer cycles without treatment and 66 with prednisone plus HCQ treatment among ANA-positive women were compared. Results There was no statistical difference in number of retrieved oocytes (13.66 ± 7.71 vs 13.72 ± 7.23, p = .445), available embryos (5.23 ± 3.37 vs 5.47 ± 3.26, p = .347), high-quality embryos (3.64 ± 3.25 vs 3.70 ± 3.52, p = .832), and proportion of high-quality embryos (26.5% vs. 26.7%, p = .940). Biochemical pregnancy rate (33.3% vs. 68.2%, p < .05), clinical pregnancy rate (20.0% vs. 50.1%, p < .05), and implantation rate (5.6% vs. 31.8%, p < .05) were lower, and pregnancy loss rate (83.3% vs. 23.1%, p < .05) was higher in patients with treatment than no treatment. Conclusion The influence of ANA on number of retrieved oocytes, available embryos, high-quality embryos, and proration of high-quality embryos was not found. The treatment of prednisone plus HCQ may improve implantation rate, biochemical pregnancy rate, and clinical pregnancy rate, and reduce pregnancy loss rate in frozen embryo transfer outcomes for ANA-positive women.

scholarly journals Effect of Human Chorionic Gonadotropin Injection Before Frozen Embryo Transfer on Pregnancy Outcomes in Endometriosis-Associated Infertility

2020 ◽  
Vol 7 ◽  
Author(s):  
Yanbo Du ◽  
Lei Yan ◽  
Mei Sun ◽  
Yan Sheng ◽  
Xiufang Li ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Birth Rate ◽  
Live Birth Rate ◽  
Abortion Rate ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Control Group

Purpose: The aim of this study was to investigate the effect of human chorionic gonadotropin (hCG) in hormone replacement (HT) regime for frozen thawed embryo transfer in women with endometriosis (EM).Methods: We performed a retrospective, database-search, cohort study and included data on EM patients who underwent frozen embryo transfer (FET) between January 1, 2009 and August 31, 2018. According to the protocols for FET cycle, the patients were divided into two groups: control group (n = 296) and hCG group (n = 355). Clinical pregnancy rate, live birth rate, early abortion rate, late abortion rate, and ectopic pregnancy rate were compared between the two groups.Results: There was a significant increase in clinical pregnancy rate in the hCG group (57.7 vs. 49%, p = 0.027) compared with the control group. The live birth rate in the hCG group (45.6 vs. 38.5%, p = 0.080) was also elevated, but this difference was not statistically significant.Conclusion: hCG administration in HT regime for FET increases the pregnancy rate in women with EM.

scholarly journals Effects of pelvic floor bioelectrical stimulation on frozen embryo transfer patients with thin endometrium

2019 ◽  
Vol 17 ◽  
pp. 205873921882286
Author(s):  
Bing Yuan ◽  
Hong Zheng ◽  
Minling Xu ◽  
Xiaoli Mu ◽  
Fengcai Huang
Keyword(s):  
Pelvic Floor ◽  
Embryo Transfer ◽  
Embryo Implantation ◽  
Implantation Rate ◽  
Frozen Embryo Transfer ◽  
Control Group ◽  
Observation Group ◽  
Thin Endometrium ◽  
Significant Difference ◽  
Artificial Cycle

This study was to observe the effects of pelvic floor bioelectrical stimulation on pregnancy outcome and serum estradiol (E2) and progesterone (P) levels in frozen embryo transfer patients with thin endometrium. 120 cases frozen embryo transfer patients with thin endometrium in our hospital from March 2016 to April 2017 were selected. These patients received artificial cycle replacement plan before embryo transfer. According to whether pelvic floor bioelectrical stimulation therapy was accepted, 120 cases of thin endometrial frozen embryo transfer were divided into control group and observation group. 50 cases of the control group received artificial cycle to prepare for embryo transplantation, while 70 cases of observation group received bioelectrical stimulation as intervention treatment on the basis of artificial cycle. The intima thickness, subintimal hemodynamic parameters, embryo implantation rate, and clinical pregnancy rate were compared between the two groups before and after treatment. There was no significant difference in endometrial thickness between the two groups on the 10th day of menstruation ( P > 0.05). The thickness of endometrium and the index of intima growth in the observation group were significantly higher than those in the control group ( P < 0.05). The pulsatility index, resistance index, and systolic and diastolic blood flow velocity ratio of subintimal blood flow in the observation group were significantly higher than those in the control group ( P < 0.05). Before treatment, there was no significant difference in serum E2 and P between the two groups ( P > 0.05). After treatment, the improvement of serum E2 and P in the observation group was significantly better than that of the control group ( P < 0.05). The embryo implantation rate (25.7%) in the observation group was significantly higher than that in the control group (17.0%) ( P < 0.05). The clinical pregnancy rate (47.1%) in the observation group was significantly higher than that in the control group (30%) ( P < 0.05). In conclusion, the intervention of pelvic floor bioelectrical stimulation can improve the blood perfusion of thin endometrium, improve the endometrial receptivity, increase the implantation rate of frozen embryo transfer in patients with thin endometrium, and improve the level of serum estradiol and progesterone.

scholarly journals Effect of human chorionic gonadotropin injection before frozen embryo transfer on pregnancy outcomes in endometriosis infertility

2020 ◽  
Author(s):  
Yanbo Du ◽  
Lei Yan ◽  
Mei Sun ◽  
Yan Sheng ◽  
Xiufang Li ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Live Birth ◽  
Birth Rate ◽  
Hormone Replacement ◽  
Live Birth Rate ◽  
Abortion Rate ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Control Group

Abstract Purpose To investigate the effect of hCG in hormone replacement regime for frozen thawed embryo transfer in women with endometriosis. Methods We performed a retrospective, database-searched cohort study. The data of endometriosis patients who underwent frozen embryo transfer between 1/1/2009-31/8/2018 were collected. According to the protocols for frozen embryo transfer cycle, these patients were divided into two groups: Control group(n=305), and hCG group(n=362). And clinical pregnancy rate, live birth rate, early abortion rate, late abortion rate and ectopic pregnancy rate were compared between the two groups. Results There was a significant increase in clinical pregnancy rate in hCG group (56.6% vs. 48.2%, p=0.035) compared to the control group. And the live birth rate in hCG group (43.5% vs. 37.4%, p=0.113) also elevated, but the difference is statistically insignificant. Conclusion hCG administration in hormone replacement regime for FET increase the pregnancy rate in women with endometriosis.

scholarly journals Frozen embryo transfer: Endometrial preparation by letrozole versus hormone replacement cycle: A randomized clinical trial

2019 ◽  
Author(s):  
Alamtaj Samsami ◽  
Leila Ghasmpour ◽  
Sara Davoodi ◽  
Shaghayegh Moradi Alamdarloo ◽  
Jamshid Rahmati ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Hormone Replacement ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Estradiol Valerate ◽  
Normal Cycle ◽  
Endometrial Preparation ◽  
Significant Difference ◽  
The Mean

Background: The endometrial preparation with stimulating natural cycles for frozen embryo transfer (FET) have benefits like lower cost and ease of use. Objective: Comparing the clinical outcome of letrozole versus hormone replacement (HR) for endometrial preparation in women with normal menstrual cycles for FET in artificial reproduction techniques. Materials and Methods: A total of 167 participants who had frozen embryos and regular ovulatory cycles were randomly divided into two groups for endometrial preparation. One group (82 women) was stimulated with letrozole 5mg/day and the other group (85 women) was hormonally stimulated by oral estradiol valerate (2 mg three times a day). All participants were followed serially by ultrasonography. Any patient who did not reach optimal endometrial thickness was excluded from the study. Implantation, biochemical and clinical pregnancy and abortion rate were reported. Results: There was no significant difference in the mean age, duration, and primary or secondary infertility, cause of the infertility, number, and quality of transferred embryos between the groups. The mean estradiol level on the day of transfer was 643 ± 217 in the HR group and 547 ± 212 in the letrozole group (P = 0.01), which was significantly different. The clinical pregnancy rate was 38.7 in the letrozole group, higher than the HR group (25.3) but not significantly different (P=0.06). Conclusion: For endometrial preparation in women with a normal cycle, letrozole yields higher pregnancy rate although it is not significant; due to its cost, ease in use, and lower side effects, letrozole is a good choice. Key words: Letrozole, Hormone replacement, Endometrial, Preparation, Frozen, Embryo.

scholarly journals The effect of catheter rotation during its withdrawal on frozen thawed embryo-transfer cycles outcomes: A Case-control study

2019 ◽  
Author(s):  
Maryam Eftekhar ◽  
Lida Saeed ◽  
Masrooreh Hoseini
Keyword(s):  
Embryo Transfer ◽  
Case Control Study ◽  
Implantation Rate ◽  
Case Control ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Control Group ◽  
Significant Difference ◽  
Control Study ◽  
Frozen Thawed Embryo Transfer

Background: Embryo transfer (ET) is the last and the most clinical process in assisted reproductive technology cycle. It has been suggested that cervical mucus interacts with an adequate embryo transfer in different ways. A few studies showed that catheter rotation could discharge mucus entrapped in the embryo to neutralize embryo displacement. Objective: The aim of this present study was to compare the outcome of frozen embryo transfer (FET) based on catheter rotation during withdrawal. Materials and Methods: In this case-control study, the clinical documents of 240 women who experienced frozen embryo transfer cycles were reviewed. The subjects were divided into two groups (n = 120/each), including A) the rotation treatment group (360°) that underwent ET using catheter rotation and B) the control group including the subjects who experienced ET with no catheter rotation. Clinical and chemical pregnancies and implantation rates were compared between two groups. Results: Results showed that there is no significant difference between the basic clinical and demographic features of both groups (p > 0.05). A significant difference was observed in terms of the rate of chemical pregnancy between groups (21.7% vs 43.3%, p = 0.001 respectively). In addition, the rate of clinical pregnancy was significantly higher in study group than the control (33.35% vs 14.2%, p = 0.002, respectively). Conclusion: Our results demonstrated that catheter rotation during withdrawal increased the implantation rate and clinical pregnancy.

scholarly journals Evaluating of the Pregnancy Rate of Freezing Embryo Transfer in the Presence or Absence of GnRH Agonist

2021 ◽  
Vol 23 (6) ◽  
pp. 818-827
Author(s):  
Leila Naserpoor ◽  
◽  
Katayoun Berjis ◽  
Rahil Jannatifar ◽  
◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Gnrh Agonist ◽  
Assisted Reproductive Technologies ◽  
Implantation Rate ◽  
Study Groups ◽  
Reproductive Technologies ◽  
Abortion Rate ◽  
Frozen Embryo Transfer ◽  
Significant Difference

Background and Aim: Applying Assisted Reproductive Technologies (ARTs) is increasing. A critical step in ART is the frozen embryo transfer, in which the endometrium thickness has great significance in the outcome. In this case, the frozen embryo will be transferred during the next cycle. There are several ways to prepare an endometrium for transmitting embryos; however, choosing the best method remains debated. The present study aimed to evaluate the pregnancy rate of frozen embryo transfer in the presence or absence of GnRH agonists. Methods & Materials: A retrospective analysis was conducted on 146 consecutive patients attending Qom’s infertility treatment center from 2015 to 2017; these subjects were candidates for the transfer cycle of the frozen-thawed embryo and randomly assigned to receive either protocol with or without GnRH agonist. Clinical features, implantation rate, pregnancy rate (chemical & clinical), and abortion rate were assessed. Ethical Considerations: This study was approved by the Research Ethics Committee of the Academic Center for Education, Culture, and Research of Mashhad University (Code: IR.ACECR.JDM.REC.1398.001). Results: There was no significant difference in baseline and clinical characteristics, implantation rate, pregnancy rate (chemical & clinical), and abortion rate between the study groups of endometrial preparations with or without GnRH agonist (P<0.05). Conclusion: In this study, pregnancy outcome was similar in both study groups; thus, this method is recommended as an endometrial preparation without GnRH agonist.

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