DNA synthesis —Dependent cell division ofEscherichia coli 15 TAU after arginine and uracil starvation

1975 ◽  
Vol 20 (1) ◽  
pp. 8-16
Author(s):  
M. Lhotská ◽  
V. Vondrejs
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Ofescherichia Coli ◽  
Dependent Cell

scholarly journals Effect of inhibition of DNA synthesis on u.v. sensitive Bs strains ofEscherichia coli

1969 ◽  
Vol 14 (2) ◽  
pp. 111-119 ◽  
Author(s):  
Michael H. L. Green ◽  
John Donch ◽  
Young S. Chung ◽  
Joseph Greenberg
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Dna Synthesis ◽  
Nalidixic Acid ◽  
Specific Inhibitor ◽  
Ofescherichia Coli ◽  
Inhibition Of Dna Synthesis ◽  
Division Mechanism

The effect of nalidixic acid, a specific inhibitor of DNA synthesis, onEscherichia colistrain B (lon) and its u.v.-sensitive derivatives is examined. Strain B itself is sensitive to nalidixic acid, whereas its u.v.-resistant derivative B/r is resistant.It is shown that in allexr Astrains, in which u.v.-induced filamentation is suppressed, resistance to nalidixic acid is increased. Amongexr Astrains, Bs4 is exceptionally resistant to nalidixic acid. This is because nalidixic acid kills only growing cells and strain Bs4, atryauxotroph, may grow poorly under the conditions used to test nalidixic acid.Theuvrgenes of the HCR strains Bs1, Bs8 and Bs12 do not suppress u.v.-induced filamentation nor do they affect the response to nalidixic acid. Theuvrgene of strain Bs3 is unusual in increasing the tendency to filament and also sensitivity to nalidixic acid.Strains Bs1, Bs3 and Bs8 are all doubly mutated from strain B, the second mutation (notuvr) being responsible for their increased resistance to nalidixic acid as well as partially or completely suppressing filamentation.It is concluded that the cell division mechanism of (lon) strain B is sensitive to inhibition of DNA synthesis. Mutations which suppress the tendency of strain B to filament reduce its sensitivity to inhibition of DNA synthesis.

Growth and cell division ofEscherichia coli 173-25 in the presence of some analogues of diaminopimelic acid

1974 ◽  
Vol 14 (4) ◽  
pp. 283-296 ◽  
Author(s):  
J. Chaloupka ◽  
M. Strnadová ◽  
J. Čáslavská ◽  
K. Vereš
Keyword(s):  
Cell Division ◽  
Ofescherichia Coli ◽  
Diaminopimelic Acid

scholarly journals Regulation of Division and Differentiation of Plant Stem Cells

2018 ◽  
Vol 34 (1) ◽  
pp. 289-310 ◽  
Author(s):  
Edith Pierre-Jerome ◽  
Colleen Drapek ◽  
Philip N. Benfey
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Gene Regulatory Networks ◽  
Regulatory Networks ◽  
Cell Types ◽  
Stem Cell Maintenance ◽  
Cell Position ◽  
Dependent Cell ◽  
Gene Regulatory ◽  
Plant Stem

A major challenge in developmental biology is unraveling the precise regulation of plant stem cell maintenance and the transition to a fully differentiated cell. In this review, we highlight major themes coordinating the acquisition of cell identity and subsequent differentiation in plants. Plant cells are immobile and establish position-dependent cell lineages that rely heavily on external cues. Central players are the hormones auxin and cytokinin, which balance cell division and differentiation during organogenesis. Transcription factors and miRNAs, many of which are mobile in plants, establish gene regulatory networks that communicate cell position and fate. Small peptide signaling also provides positional cues as new cell types emerge from stem cell division and progress through differentiation. These pathways recruit similar players for patterning different organs, emphasizing the modular nature of gene regulatory networks. Finally, we speculate on the outstanding questions in the field and discuss how they may be addressed by emerging technologies.

Inhibition of DNA synthesis and cell division by a cell surface sialoglycopeptide

1989 ◽  
Vol 139 (2) ◽  
pp. 269-274 ◽  
Author(s):  
Heideh Fattaey ◽  
Terry C. Johnson ◽  
Hsin-Hwei Chou
Keyword(s):  
Cell Division ◽  
Cell Surface ◽  
Dna Synthesis ◽  
A Cell ◽  
Inhibition Of Dna Synthesis

scholarly journals Dem Anfang auf der Spur — die Suche nach DNA-Replikationsursprüngen

BIOspektrum ◽  
2021 ◽  
Vol 27 (3) ◽  
pp. 246-249
Author(s):  
Elisabeth Kruse ◽  
Stephan Hamperl
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Genomic Dna ◽  
Genetic Material ◽  
Uniform Method ◽  
Replication Origins ◽  
Daughter Cells ◽  
Origins Of Replication ◽  
Mammalian Genomes ◽  
Comprehensive Manner

AbstractTimely and accurate duplication of DNA prior to cell division is a prerequisite for propagation of the genetic material to both daughter cells. DNA synthesis initiates at discrete sites, termed replication origins, and proceeds in a bidirectional manner until all genomic DNA is replicated. Despite the fundamental nature of these events, a uniform method that identifies origins of replication in a comprehensive manner is still missing. Here, we present currently available and discuss new approaches to map replication origins in mammalian genomes.

Changes of the DNA content of differentiating and adult macronuclei of the ciliate Loxodes magnus (Karyorelictida)

1980 ◽  
Vol 44 (1) ◽  
pp. 375-394
Author(s):  
N.N. Bobyleva ◽  
B.N. Kudrjavtsev ◽  
I.B. Raikov
Keyword(s):  
Cell Division ◽  
Dna Replication ◽  
Hydrochloric Acid ◽  
Dna Synthesis ◽  
Acid Hydrolysis ◽  
Gene Amplification ◽  
Dna Content

The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear Anlagen proved to be the same as that of telophasic micronuclei (2 c). The Anlagen thus differentiate from micronuclei which are still in G1. The quantity of DNA in the macronuclear Anlagen thereafter rises to the 4-c level, simultaneously with DNA replication in the micronuclei which immediately follows mitosis. In non-dividing animals most micronuclei are already in G2. Adult macronuclei here contain on average 1.5 times more DNA than the micronuclei; their DNA content is about 5–6 c (in some individual nuclei, up to 10 c). These data are consistent with autoradiographic evidence indicating a weak DNA synthesis in the macronuclei of Loxodes and make likely the existence of partial DNA replication (e.g. gene amplification) in the macronuclei. The DNA content of adult macronuclei isolated from dividing animals proved to be significantly smaller than that of macronuclei isolated from non-dividing specimens of the same clone. In 3 clones studied, the former value amounted on average to 71–79, 78 and 95% of the latter, respectively. This drop of DNA content cannot be explained by ‘dilution’ of the old macronuclei with newly formed ones. The quantity of DNA in adult macronuclei thus seems to undergo cyclical changes correlated with cytokinesis, despite the fact that, in Loxodes magnus, the macronuclei themselves never divide and are simply segregated at every cell division. The macronuclei of Loxodes can be termed paradiploid or hyperdiploid.

Synchronous DNA synthesis following heat-synchronized cell division in Tetrahymena

1967 ◽  
Vol 48 (1) ◽  
pp. 231-232 ◽  
Author(s):  
K.K. Hjelm ◽  
E. Zeuthen
Keyword(s):  
Cell Division ◽  
Dna Synthesis
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