The mutagenic effect of ultraviolet radiation and nitrous acid onMycobacterium phlei

1966 ◽  
Vol 11 (3) ◽  
pp. 163-168 ◽  
Author(s):  
Miluše Radochová ◽  
J. Koníček ◽  
I. Málek
Keyword(s):  
Ultraviolet Radiation ◽  
Nitrous Acid ◽  
Mutagenic Effect

Mutagenic effect of ultraviolet radiation on the non-acid-fast strain ofMycobacterium phlei

1968 ◽  
Vol 13 (4) ◽  
pp. 282-287 ◽  
Author(s):  
J. Koníček ◽  
I. Málek
Keyword(s):  
Ultraviolet Radiation ◽  
Mutagenic Effect ◽  
Strain Ofmycobacterium

COCHLIOBOLUS SATIVUS: III. EFFECT OF ULTRAVIOLET RADIATION

1960 ◽  
Vol 38 (3) ◽  
pp. 275-282 ◽  
Author(s):  
R. D. Tinline ◽  
J. F. Stauffer ◽  
J. G. Dickson
Keyword(s):  
Ultraviolet Radiation ◽  
Ultraviolet Light ◽  
Aerial Mycelium ◽  
Mutagenic Effect ◽  
Cochliobolus Sativus ◽  
Highly Pathogenic ◽  
Wheat Seedlings ◽  
Rate Of Growth ◽  
High Doses ◽  
Sporulation Rate

The lethal and mutagenic effect of ultraviolet light on two isolates of Cochliobolus sativus was studied. Conidia of a dark-spored strain were considerably more resistant to high doses of radiation than those of a white-spored strain. Ultraviolet radiation increased the frequency of mutation in the fungus. Mutants were recovered that differed from their parents in degree of sporulation, rate of growth, pigmentation, amount of aerial mycelium, topography and consistency of colony, and pathogenicity to wheat seedlings. Tetrads from crosses between weakly and highly pathogenic strains indicated that pathogenicity was not a clearly segregating character.

scholarly journals CROSS-RESISTANCE RELATIONSHIPS IN ESCHERICHIA COLI BETWEEN ULTRAVIOLET RADIATION AND NITROUS ACID

1964 ◽  
Vol 87 (5) ◽  
pp. 1094-1099 ◽  
Author(s):  
Antonio Zampieri ◽  
Joseph Greenberg
Keyword(s):  
Escherichia Coli ◽  
Ultraviolet Radiation ◽  
Nitrous Acid ◽  
Cross Resistance

Studies on the inactivating and mutagenic effect of nitrous acid and hydroxylamine on viruses

1961 ◽  
Vol 58 ◽  
pp. 1005-1010 ◽  
Author(s):  
Heinz Schuster ◽  
Walter Vielmetter
Keyword(s):  
Nitrous Acid ◽  
Mutagenic Effect

Possible interpretation of the mutagenic effect of ultraviolet radiation

1964 ◽  
Vol 6 (2) ◽  
pp. 201-207 ◽  
Author(s):  
J. Ladik
Keyword(s):  
Ultraviolet Radiation ◽  
Mutagenic Effect

Applications of Photoelectron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 506-507
Author(s):  
William J. Baxter
Keyword(s):  
Electron Microscopy ◽  
Thermal Decomposition ◽  
Chemical Composition ◽  
Ultraviolet Radiation ◽  
Thin Layer ◽  
Edge Effects ◽  
Electron Optics ◽  
Surface Layers ◽  
Crystalline Orientation ◽  
Fluorescent Screen

In this form of electron microscopy, photoelectrons emitted from a metal by ultraviolet radiation are accelerated and imaged onto a fluorescent screen by conventional electron optics. image contrast is determined by spatial variations in the intensity of the photoemission. The dominant source of contrast is due to changes in the photoelectric work function, between surfaces of different crystalline orientation, or different chemical composition. Topographical variations produce a relatively weak contrast due to shadowing and edge effects.Since the photoelectrons originate from the surface layers (e.g. ∼5-10 nm for metals), photoelectron microscopy is surface sensitive. Thus to see the microstructure of a metal the thin layer (∼3 nm) of surface oxide must be removed, either by ion bombardment or by thermal decomposition in the vacuum of the microscope.

Deformation of Yeast Plasma Membrane by Ethidium Bromide

1988 ◽  
Vol 46 ◽  
pp. 254-255
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Thin Section ◽  
Ethidium Bromide ◽  
Freeze Fracture ◽  
Mutagenic Effect ◽  
Yeast Cells ◽  
Hydrophobic Region ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

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