Transformation of japonica rice withRHL gene and salt tolerance of the transgenic rice plant

2002 ◽  
Vol 47 (12) ◽  
pp. 998-1002 ◽  
Author(s):  
Rongtian Li ◽  
Zhongming Zhang ◽  
Qifa Zhang
2020 ◽  
Vol 8 (2) ◽  
pp. 131-140
Author(s):  
So Young Kim ◽  
Hyemin Lim ◽  
Min Kang ◽  
Kyong Mi Jun ◽  
Seung Uk Ji ◽  
...  

Incorporated of CsNitr1-L-transgene in rice plant was showed to active and transport NO2 - from cytosol into chloroplast. With a PCR method screening of CsNitr1-L gene were confirmed which the CsNitr1-L-transgene have amplification band, while the plant were not contained CsNitr1-L gene, there are no amplification band. Some strain were obtained and got a seeds. Almost CsNitr1-L-transgene was raising in all parameter test, such as plant height, tillers number, respectively. The accumulation of NO2 - in the leaves of CsNitr1-L-transgene was lower compared with wild type. CsNitr1-L is a cucumber NO2 - -transporter gene that located in chloroplast membrane have been transformed into japonica rice cv. Nipponbare. A series experiments was carried out to understand the expression- CsNitr1-L-transgenic rice. The seeds were cultivated to observe the activity of CsNitr1-L-transgene with medium containing NO3 - or NO2 - as inducer. Agronomical test, like tillers number and plant height were observed and compare with non CsNitr1-L- transgenic rice. While the NO2 - content in leaves were analyzed with capillary electrophoresis method


2010 ◽  
Vol 51 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Naoki Wada ◽  
Shin'ichiro Kajiyama ◽  
Joyce A. Cartagena ◽  
Linyen Lin ◽  
Yukio Akiyama ◽  
...  

2000 ◽  
Vol 3 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Wataru Takahashi ◽  
Takiko Shimada ◽  
Junjo Matsushita ◽  
Osamu Tanaka

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