Flow sorting of wheat chromosome arms from the ditelosomic line 7BL

2006 ◽  
Vol 24 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Dong-Wei Guo ◽  
You-Zhi Ma ◽  
Lian-Cheng Li ◽  
Yao-Feng Chen
Keyword(s):  
Wheat Chromosome ◽  
Flow Sorting ◽  
Ditelosomic Line

Mantle origin and flow sorting of megacryst-xenolith inclusions in mafic dikes of Black Canyon, Arizona

1994 ◽  
Author(s):  
J.E. Nielson ◽  
J.K. Nakata
Keyword(s):  
Mantle Origin ◽  
Flow Sorting

Linkage Relationships among Genes on Wheat Chromosome 5A That Condition Resistance to Hessian Fly

Crop Science ◽  
1995 ◽  
Vol 35 (6) ◽  
pp. 1603-1607 ◽  
Author(s):  
H. W. Ohm ◽  
H. C. Sharma ◽  
F. L. Patterson ◽  
R. H. Ratcliffe ◽  
M. Obanni
Keyword(s):  
Wheat Chromosome ◽  
Hessian Fly ◽  
Chromosome 5A ◽  
Linkage Relationships

scholarly journals A wheat chromosome 5AL region confers seedling resistance to both tan spot and Septoria nodorum blotch in two mapping populations

2019 ◽  
Vol 7 (6) ◽  
pp. 809-818 ◽  
Author(s):  
Wenjing Hu ◽  
Xinyao He ◽  
Susanne Dreisigacker ◽  
Carolina P. Sansaloni ◽  
Philomin Juliana ◽  
...  
Keyword(s):  
Wheat Chromosome ◽  
Tan Spot ◽  
Septoria Nodorum ◽  
Seedling Resistance ◽  
Septoria Nodorum Blotch ◽  
Mapping Populations

scholarly journals A Duplicated Copy of the Meiotic Gene ZIP4 Preserves up to 50% Pollen Viability and Grain Number in Polyploid Wheat

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 290
Author(s):  
Abdul Kader Alabdullah ◽  
Graham Moore ◽  
Azahara C. Martín
Keyword(s):  
Pollen Viability ◽  
Pollen Grains ◽  
Wheat Chromosome ◽  
Grain Number ◽  
Meiotic Gene ◽  
Chromosome 5B ◽  
Mutant Pollen ◽  
Female Gametogenesis ◽  
Chromosome 3B

Although most flowering plants are polyploid, little is known of how the meiotic process evolves after polyploidisation to stabilise and preserve fertility. On wheat polyploidisation, the major meiotic gene ZIP4 on chromosome 3B duplicated onto 5B and diverged (TaZIP4-B2). TaZIP4-B2 was recently shown to promote homologous pairing, synapsis and crossover, and suppress homoeologous crossover. We therefore suspected that these meiotic stabilising effects could be important for preserving wheat fertility. A CRISPR Tazip4-B2 mutant was exploited to assess the contribution of the 5B duplicated ZIP4 copy in maintaining pollen viability and grain setting. Analysis demonstrated abnormalities in 56% of meiocytes in the Tazip4-B2 mutant, with micronuclei in 50% of tetrads, reduced size in 48% of pollen grains and a near 50% reduction in grain number. Further studies showed that most of the reduced grain number occurred when Tazip4-B2 mutant plants were pollinated with the less viable Tazip4-B2 mutant pollen rather than with wild type pollen, suggesting that the stabilising effect of TaZIP4-B2 on meiosis has a greater consequence in subsequent male, rather than female gametogenesis. These studies reveal the extraordinary value of the wheat chromosome 5B TaZIP4-B2 duplication to agriculture and human nutrition. Future studies should further investigate the role of TaZIP4-B2 on female fertility and assess whether different TaZIP4-B2 alleles exhibit variable effects on meiotic stabilisation and/or resistance to temperature change.

scholarly journals Localization of Male-Specifically Expressed MROS Genes of Silene latifolia by PCR on Flow-Sorted Sex Chromosomes and Autosomes

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1269-1277
Author(s):  
Eduard Kejnovský ◽  
Jan Vrána ◽  
Sachihiro Matsunaga ◽  
Přemysl Souček ◽  
Jiří Široký ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Silene Latifolia ◽  
Homology Search ◽  
X Chromosomes ◽  
Dioecious Plants ◽  
Flow Sorting ◽  
Heteromorphic Sex Chromosomes ◽  
Copy Gene ◽  
Melandrium Album ◽  
Pcr Products

Abstract The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY in males, that are larger than the autosomes and enable their separation by flow sorting. The group of MROS genes, the first male-specifically expressed genes in dioecious plants, was recently identified in S. latifolia. To localize the MROS genes, we used the flow-sorted X chromosomes and autosomes as a template for PCR with internal primers. Our results indicate that the MROS3 gene is located in at least two copies tandemly arranged on the X chromosome with additional copy(ies) on the autosome(s), while MROS1, MROS2, and MROS4 are exclusively autosomal. The specificity of PCR products was checked by digestion with a restriction enzyme or reamplification using nested primers. Homology search of databases has shown the presence of five MROS3 homologues in A. thaliana, four of them arranged in two tandems, each consisting of two copies. We conclude that MROS3 is a low-copy gene family, connected with the proper pollen development, which is present not only in dioecious but also in other dicot plant species.

Incidence of chromosome aberrations in mammalian sperm stained with Hoechst 33342 and UV-laser irradiated during flow sorting

1987 ◽  
Vol 182 (5) ◽  
pp. 265-274 ◽  
Author(s):  
B.L. Libbus ◽  
S.D. Perreault ◽  
L.A. Johnson ◽  
D. Pinkel
Keyword(s):  
Chromosome Aberrations ◽  
Uv Laser ◽  
Hoechst 33342 ◽  
Mammalian Sperm ◽  
Flow Sorting

Flow sorting and culture of protoplasts: Conditions for high-frequency recovery, growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana

1984 ◽  
Vol 3 (4) ◽  
pp. 151-155 ◽  
Author(s):  
D. W. Galbraith ◽  
C. L. Afonso ◽  
K. R. Harkins
Keyword(s):  
High Frequency ◽  
Suspension Cultures ◽  
Flow Sorting

Layered Granitic Rocks at Chebucto Head, Halifax County, Nova Scotia

1975 ◽  
Vol 12 (3) ◽  
pp. 456-463 ◽  
Author(s):  
T. E. Smith
Keyword(s):  
Grain Size ◽  
Nova Scotia ◽  
Granitic Rocks ◽  
Textural Characteristics ◽  
Rock Types ◽  
Flow Sorting

Structural, mineralogical and textural characteristics of some layered granitic rocks are described. The layers result from the segregation of minerals into bands and lenses of contrasting color and grain size. They were formed near the roof of the pluton prior to final crystallization by flow sorting during episodic shearing generated during intrusion. Comparison of the mineralogy and textures of the layered rocks with those of the main rock types of the pluton shows that the differentiation of the pluton as a whole took place after the formation of the layers by interaction of felsic components concentrated in residual liquids with earlier formed crystals.

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