Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system

1993 ◽  
Vol 64 (1) ◽  
pp. 137-144 ◽  
Author(s):  
Ruth Knuechel ◽  
Markus Burgau ◽  
Josef Rueschoff ◽  
Ferdinand Hofstaedter
2015 ◽  
Vol 6 (1) ◽  
pp. 265-270
Author(s):  
Kai Zhang ◽  
Chuanfu Li ◽  
Ying Liu ◽  
Li Li ◽  
Xiangshui Meng ◽  
...  

AbstractThe purpose of this study was to analyze if there is a significant correlation between the results of diffusionweighted imaging (DWI) and the expression of proliferating cell nuclear antigen (PCNA) in astrocytomas. The DWI scans of 19 different-grade astrocytomas were obtained on a 3 T magnetic resonance scanner. The average regional apparent diffusion coefficients (ADC) were measured. The positive expression of PCNA was determined immunohistochemically by using streptavidin-peroxidase complex staining, and was quantified by calculating its calibrated opacity density (COD) using an image analysis system. The average regional ADC and PCNA COD of low grade and high grade astrocytomas were compared. Correlations between regional ADC and PCNA COD were analyzed. The average regional ADC of high grade astrocytomas was significantly (t = 10.169, P = 0.000) less (0.687 ± 0.225 x 10-3 mm2/s) than that of low grade astrocytomas (1.572 ± 0.333 x 10-3 mm2/s). The PCNA COD (0.343 ± 0.052) of high grade astrocytomas was significantly (t=-7.858, P=0.000) greater than that (0.194 ± 0.012) of low grade astrocytomas. There were strong negative correlations between regional ADC and PCNA COD (r = -0.801, P = 0.000). The results demonstrated that DWI is helpful in evaluating cell proliferation and preoperatively grading astrocytomas by measuring regional ADC.


1994 ◽  
Vol 42 (3) ◽  
pp. 321 ◽  
Author(s):  
E Mapfumo

The magnitude of error in estimating axial resistances was assessed using the diameters of xylem vessels of different shapes, measured using four different methods. Statistical analyses showed that the diameter measurements obtained using the different methods, were significantly different at the 0.001 level of significance. Using the Tukey test, the diameters obtained using the 'four axes' method were not significantly different from the actual diameters obtained using the Chromatic Color Image Analysis System. By using the 'Lewis' method for measuring the xylem diameter, axial resistance was overestimated by a factor of 1.6. Thus, the error caused by the method of measurement may partly explain why, in some studies, the estimated axial resistances have always been different from the measured resistances. The 'four axes' method is the simplest and most reliable and gives results very close to the actual diameters, compared with the other three methods.


1989 ◽  
Author(s):  
R. J. Madachy ◽  
L. Chotivichit ◽  
H. K. Huang ◽  
E. E. Johnson

1994 ◽  
Vol 37 (2) ◽  
pp. 303-313 ◽  
Author(s):  
Sandra L. Ettema ◽  
David P. Kuehn

This study was designed to re-examine the same soft palates studied qualitatively in a previous investigation (Kuehn and Kahane, 1990), using a true-color image analysis system. The quantified measures of the areas of specific tissue types are reported. The results indicate that: (a) tendinous tissue is prominent anteriorly and comprises about 10% of total tissue in that region, (b) the relative proportion of glandular and connective tissue is fairly uniform across the length of the soft palate, averaging 22% and 36% respectively, (c) muscle tissue shows a pattern of increasing then decreasing amount from anterior to posterior with a maximum proportion of 23% in the midportion of the soft palate, (d) adipose tissue comprises 22% of total tissue area in the anterior segment and 17% of total tissue area in the posterior segment of the soft palate, (e) other tissue, primarily epithelium and vascular tissue, was found to be fairly constant anteriorly and increased in relative proportion to almost 30% of the total tissue area at the uvular base. The functional implications of these findings are discussed.


1990 ◽  
Vol 61 (11) ◽  
pp. 984-989
Author(s):  
Keigo KUCHIDA ◽  
Kuniji YAMAKI ◽  
Toshihiro YAMAGISHI ◽  
Yutaka MIZUMA ◽  
Kouzou SATOH

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