Evaluation of astrocytoma cell proliferation using diffusion-weighted imaging: correlation with expression of proliferating cell nuclear antigen

AbstractThe purpose of this study was to analyze if there is a significant correlation between the results of diffusionweighted imaging (DWI) and the expression of proliferating cell nuclear antigen (PCNA) in astrocytomas. The DWI scans of 19 different-grade astrocytomas were obtained on a 3 T magnetic resonance scanner. The average regional apparent diffusion coefficients (ADC) were measured. The positive expression of PCNA was determined immunohistochemically by using streptavidin-peroxidase complex staining, and was quantified by calculating its calibrated opacity density (COD) using an image analysis system. The average regional ADC and PCNA COD of low grade and high grade astrocytomas were compared. Correlations between regional ADC and PCNA COD were analyzed. The average regional ADC of high grade astrocytomas was significantly (t = 10.169, P = 0.000) less (0.687 ± 0.225 x 10-3 mm2/s) than that of low grade astrocytomas (1.572 ± 0.333 x 10-3 mm2/s). The PCNA COD (0.343 ± 0.052) of high grade astrocytomas was significantly (t=-7.858, P=0.000) greater than that (0.194 ± 0.012) of low grade astrocytomas. There were strong negative correlations between regional ADC and PCNA COD (r = -0.801, P = 0.000). The results demonstrated that DWI is helpful in evaluating cell proliferation and preoperatively grading astrocytomas by measuring regional ADC.

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Proliferating cell nuclear antigen assessed by a computer-assisted image analysis system in patients with chronic viral hepatitis and cirrhosis

Digestive and Liver Disease ◽

10.1016/s1590-8658(02)80193-1 ◽

2002 ◽

Vol 34 (3) ◽

pp. 197-203 ◽

Cited By ~ 10

Author(s):

M.F Donato ◽

E. Arosio ◽

V. Monti ◽

P. Fasani ◽

D. Prati ◽

...

Keyword(s):

Image Analysis ◽

Viral Hepatitis ◽

Proliferating Cell Nuclear Antigen ◽

Chronic Viral Hepatitis ◽

Nuclear Antigen ◽

Computer Assisted ◽

Image Analysis System ◽

Computer Assisted Image ◽

Analysis System ◽

Proliferating Cell

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Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system

Virchows Archiv B Cell Pathology Including Molecular Pathology ◽

10.1007/bf02915106 ◽

1993 ◽

Vol 64 (1) ◽

pp. 137-144 ◽

Cited By ~ 7

Author(s):

Ruth Knuechel ◽

Markus Burgau ◽

Josef Rueschoff ◽

Ferdinand Hofstaedter

Keyword(s):

Proliferating Cell Nuclear Antigen ◽

Color Image ◽

Nuclear Antigen ◽

Image Analysis System ◽

Color Image Analysis ◽

True Color Image ◽

Analysis System ◽

True Color ◽

Proliferating Cell ◽

Normal Urothelium

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Proliferating cell nuclear antigen expression in mucoepidermoid carcinoma of salivary glands

Sao Paulo Medical Journal ◽

10.1590/s1516-31802000000300004 ◽

2000 ◽

Vol 118 (3) ◽

pp. 69-74 ◽

Cited By ~ 17

Author(s):

Weder Pereira Cardoso ◽

Odilon Victor Porto Denardin ◽

Abrão Rapoport ◽

Vera Cavalcanti Araújo ◽

Marcos Brasilino Carvalho

Keyword(s):

Cell Proliferation ◽

Salivary Glands ◽

Proliferating Cell Nuclear Antigen ◽

Mucoepidermoid Carcinoma ◽

Antigen Expression ◽

Sao Paulo ◽

Nuclear Antigen ◽

High Grade ◽

Grade Of Malignancy ◽

Proliferating Cell

CONTEXT: Among the cytological and morphological properties of mucoepidermoid carcinoma, one of the most important criteria for measuring its biological behavior and aggressiveness is cell proliferation. In this way, immunohistochemical markers of cell proliferation have been found to be useful in tumor classification and have formed part of the prognostic and therapeutic studies of these pathologies. OBJECTIVE: To analyze 11 cases of mucoepidermoid carcinoma (MEC) using the proliferation activity marker (PCNA) and to determine its relationship to the grade of malignancy of these tumors. DESIGN: Correlation study. SETTING: Head and Neck Surgery Service of Heliópolis Hospital, São Paulo, Brazil. SAMPLE: Slides of 11 cases of primary mucoepidermoid carcinomas of salivary glands were prepared according to routine techniques employed in the Oral Pathology Department of the Dentistry Faculty of São Paulo University, Brazil. They were fixed in a 10% formaldehyde solution and stained with hematoxylin and eosin. After this preparation the tumors were classified as low, intermediate and high grade of malignancy, according to the criteria established by Seifert & Sobin and Auclair, Goode & Ellis. The slides were sent for immunohistochemical processing to evaluate the positivity of proliferating cell nuclear antigen using the streptavidin biotin technique. MAIN MEASUREMENT: The correlation between proliferating cell nuclear antigen expression and the histological malignancy grade in mucoepidermoid carcinoma of salivary glands. RESULTS: there were 4 cases (36%) of low grade, 4 cases (36%) of intermediate grade and 3 cases (27%) of high grade of malignancy. After a comparative study between histological features and immunohistochemical analysis, significant differences were observed (P < 0.01) for low, intermediate and high grades: 16.04%, 26.98% and 56.98% of proliferating cell nuclear antigen expression in mucoepidermoid carcinoma , respectively. CONCLUSION: The proliferating cell nuclear antigen expression increases with the grade of malignancy in mucoepidermoid carcinoma of salivary glands.

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