Effect of root tip amputation on spiral oscillations of the growing hypocotyl with radicle of the pea (Pisum sativum L.)

Bisphenol A (BPA) is a global transpiring pollutant and an endocrine disruptor present in the environment which has a substantial harmful effect on plants. In the present study, its effects on seed germination, radicle length and cytogenetic alterations were investigated in Pisum sativum L root tip cells. Pisum sativum L seeds were germinated after treating with various concentrations of BPA (2 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, 20 mg/L and 25 mg/L) at 24±1°C for 72 hours and the cytogenetic variations were assessed. The investigation showed that BPA reduced the percentage of seed germination, mitotic index, radicle length (at higher concentrations) and instigated a rise in chromosomal anomalies in a dose-related manner. In total, there is an enhanced occurrence of c-mitosis, stickiness, bridges, fragments and laggards in the BPA treated root tip cells of Pisum sativum L seeds.

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Altered growth and root tip morphology in Pisum sativum L. in response to altered expression of a gene expressed in border cells

Plant and Soil ◽

10.1007/s11104-013-1995-3 ◽

2013 ◽

Vol 377 (1-2) ◽

pp. 179-187 ◽

Cited By ~ 1

Author(s):

Fushi Wen ◽

Lindy A. Brigham ◽

Gilberto Curlango-Rivera ◽

Zhongguo Xiong ◽

Martha C. Hawes

Keyword(s):

Pisum Sativum ◽

Root Tip ◽

Border Cells ◽

Altered Expression ◽

Pisum Sativum L

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EFFECTS OF 60COBALT IONIZING IRRADIATION ON GROWTH AND POLYSOME CONTENT IN PISUM SATIVUM L. `ALASKA'

HortScience ◽

10.21273/hortsci.27.12.1261d ◽

1992 ◽

Vol 27 (12) ◽

pp. 1261d-1261

Author(s):

Jody A. Goodrich

Keyword(s):

Protein Synthesis ◽

Pisum Sativum ◽

Plant Cells ◽

Root Tip ◽

Nuclear Accidents ◽

Ionizing Irradiation ◽

Pisum Sativum L ◽

Living Organisms ◽

Injurious Effects

This research tested the hypothesis that 60Co ionizing irradiation degrades polysomes to monosomes, a process that reduces growth of Pisum sativum seedlings. Dry and imbibed seeds and 5-day-old seedlings were exposed to 1.8, 3.6, 7.2, 14.4, or 28.8 krad of 60Co irradiation. Immediately after irradiation treatments, dry and imbibed seeds were planted, and later seedlings were harvested and analyzed. Five 1-cm root-tip samples from 5-day-old seedlings were crushed and layered onto 15% to 60% sucrose gradients and centrifuged for 55 min. The samples were processed through an ISCO ultraviolet chart maker. The monosome and polysome weights were read and analyzed. The monosome content was greater in the irradiated 5-day-old seedlings than in seedlings from dry and imbibed seeds. The growth of 5-day-old irradiated seedlings and seedlings from imbibed seeds was less than that of seedlings from dry seeds. The reduced growth of the irradiated seedlings suggests damage to the polysomes. When protein synthesis in plant cells is altered, perhaps through RNA decoding mechanisms, growth may be partially or completely arrested. Using sensitive plants to establish the injurious effects of ionizing irradiation on living organisms can educate and alert society to the detrimental effects of overexposure to irradiation such as that caused by nuclear accidents.

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