Impact of Bisphenol A on seed germination, radicle length and cytogenetic alterations in Pisum sativum L

Bisphenol A (BPA) is a global transpiring pollutant and an endocrine disruptor present in the environment which has a substantial harmful effect on plants. In the present study, its effects on seed germination, radicle length and cytogenetic alterations were investigated in Pisum sativum L root tip cells. Pisum sativum L seeds were germinated after treating with various concentrations of BPA (2 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, 20 mg/L and 25 mg/L) at 24±1°C for 72 hours and the cytogenetic variations were assessed. The investigation showed that BPA reduced the percentage of seed germination, mitotic index, radicle length (at higher concentrations) and instigated a rise in chromosomal anomalies in a dose-related manner. In total, there is an enhanced occurrence of c-mitosis, stickiness, bridges, fragments and laggards in the BPA treated root tip cells of Pisum sativum L seeds.

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Impact of Engineered Si Nanoparticles on Seed Germination, Vigour Index and Genotoxicity Assessment via DNA Damage of Root Tip Cells in Lens culinaris

Journal of Plant Biochemistry & Physiology ◽

10.4172/2329-9029.1000218 ◽

2018 ◽

Vol 06 (03) ◽

Cited By ~ 1

Author(s):

Zeba Khan

Keyword(s):

Dna Damage ◽

Seed Germination ◽

Lens Culinaris ◽

Root Tip ◽

Si Nanoparticles ◽

Vigour Index ◽

Tip Cells ◽

Root Tip Cells

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Cytological changes induced by clethodim in Pisum sativum plant

Bangladesh Journal of Botany ◽

10.3329/bjb.v49i2.49318 ◽

2020 ◽

Vol 49 (2) ◽

pp. 367-374

Author(s):

Sazada Siddiqui ◽

Sulaiman Alrumman

Keyword(s):

Seed Germination ◽

Pisum Sativum ◽

Mitotic Index ◽

Cytotoxic Effect ◽

Chromosomal Anomalies ◽

Dependent Manner ◽

Cytological Changes ◽

Dose Dependent ◽

Radicle Length ◽

Mitotic Cells

Effects of clethodim treatment on mitotic cells of P. sativum were studied in the present investigation. Pisum sativum seeds are treated with different concentrations of clethodim varying from 0.01, 0.02, 0.03 and 0.04% for 1 and 2 hrs and their effect on seed germination, radicle length, mitotic index and chromosomal anomalies were investigated. The outcomes reveal that treatment of clethodim on P. sativum seeds diminishes seed germination, radicle length, and mitotic index in dose-dependent manner. Also, in clethodim treated seeds which are time and concentration dependent, an escalation in the proportion of abnormal mitotic phases was observed. C-mitosis, fragments, precocious separation, stickiness and bridges were the most frequently observed deviations. The results revealed that frequently used herbicide clethodim has a substantial cytotoxic effect on P. sativum.

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The arrangement of Pisum chromosomes in interphase

Genome ◽

10.1139/g88-119 ◽

1988 ◽

Vol 30 (5) ◽

pp. 717-722 ◽

Cited By ~ 4

Author(s):

Gisela Wolff ◽

Hans-Dietrich Quednau

Keyword(s):

Pisum Sativum ◽

Root Tip ◽

Homologous Chromosomes ◽

Multiple Tests ◽

Nucleolar Organizing Region ◽

Tests Of Significance ◽

Chromosome Position ◽

Somatic Pairing ◽

Tip Cells ◽

Root Tip Cells

Root tip cells of Pisum sativum were analyzed for the position of two chromosomes (4 and 7) that carry the nucleolar organizing region. Because of a characteristic heterochromatic pattern of these chromosomes, they can be identified in interphase. The positions of these regions were measured in relation to the center of the nucleus and to one another. The values were statistically evaluated by means of multiple tests of significance. The results discussed show clearly that in P. sativum cells the distribution of the nucleolar organizing chromosomes is not random and that the chromosomes analysed occupy special sites. There was no evidence that homologous chromosomes are more adjacent than heterologous ones, nor that morphologically similar chromosomes are neighbours. The present investigations have also shown that the position of the chromosomes is altered if the structure of the chromosomes is changed by reciprocal translocations.Key words: chromosome position, somatic pairing, translocation, heterochromatin, C-banding, multiple tests of significance.

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The effects of nano-TiO2 on seed germination, development and mitosis of root tip cells of Vicia narbonensis L. and Zea mays L

Journal of Nanoparticle Research ◽

10.1007/s11051-010-0135-8 ◽

2010 ◽

Vol 13 (6) ◽

pp. 2443-2449 ◽

Cited By ~ 172

Author(s):

Monica Ruffini Castiglione ◽

Lucia Giorgetti ◽

Chiara Geri ◽

Roberto Cremonini

Keyword(s):

Zea Mays ◽

Seed Germination ◽

Zea Mays L ◽

Root Tip ◽

Nano Tio2 ◽

Vicia Narbonensis ◽

Tip Cells ◽

Root Tip Cells

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Structural Evidence of Programmed Cell Death Induction by Tungsten in Root Tip Cells of Pisum sativum

Plants ◽

10.3390/plants8030062 ◽

2019 ◽

Vol 8 (3) ◽

pp. 62 ◽

Cited By ~ 2

Author(s):

Ioannis-Dimosthenis Adamakis ◽

Eleftherios Eleftheriou

Keyword(s):

Cell Death ◽

Pisum Sativum ◽

Programmed Cell Death ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Root Tip ◽

Confocal Laser ◽

Golgi Bodies ◽

Tip Cells ◽

Root Tip Cells

Previous studies have shown that excess tungsten (W), a rare heavy metal, is toxic to plant cells and may induce a kind of programmed cell death (PCD). In the present study we used transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) to investigate the subcellular malformations caused by W, supplied as 200 mg/L sodium tungstate (Na2WO4) for 12 or 24 h, in root tip cells of Pisum sativum (pea), The objective was to provide additional evidence in support of the notion of PCD induction and the presumed involvement of reactive oxygen species (ROS). It is shown ultrastructurally that W inhibited seedling growth, deranged root tip morphology, induced the collapse and deformation of vacuoles, degraded Golgi bodies, increased the incidence of multivesicular and multilamellar bodies, and caused the detachment of the plasma membrane from the cell walls. Plastids and mitochondria were also affected. By TEM, the endoplasmic reticulum appeared in aggregations of straight, curved or concentric cisternae, frequently enclosing cytoplasmic organelles, while by CLSM it appeared in bright ring-like aggregations and was severely disrupted in mitotic cells. However, no evidence of ROS increase was obtained. Overall, these findings support the view of a W-induced vacuolar destructive PCD without ROS enhancement.

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