In vitro studies on uptake, storage and disappearance of norepinephrine in spleen of white leghorn chicken

1989 ◽  
Vol 98 (1) ◽  
pp. 35-39
Author(s):  
Chandana Choudhury ◽  
Sushma Rishi ◽  
B D Garg
Keyword(s):  
In Vitro Studies ◽  
White Leghorn ◽  
White Leghorn Chicken

The mitotic pattern of the embryonic epidermis of chick during scale morphogenesis

Development ◽  
1969 ◽  
Vol 21 (2) ◽  
pp. 235-242
Author(s):  
Takesi Yohro
Keyword(s):  
Chick Embryo ◽  
Experimental Studies ◽  
Gross Anatomy ◽  
Epidermal Cells ◽  
Mitotic Count ◽  
Proliferation Rate ◽  
In Vitro Studies ◽  
White Leghorn ◽  
Developmental Problems

The epidermis of the chick embryo has been widely used for in vitro studies of many developmental problems (Matoltsy, 1960; Billingham & Silvers, 1963). The present attempt to determine the proliferation rate of chick embryonic epidermal cells was expected to provide a base for experimental studies, but a preliminary mitotic count revealed that the number of mitoses varied greatly in different areas. This suggested accumulation of mitoses in some restricted parts of the epidermis, and so a mapping experiment was carried out to determine the distribution of mitoses in this material. The characteristic mitotic pattern which was discovered is described and discussed. About 300 White Leghorn embryos were used: 20 for study of the gross anatomy of scales, 200 for Colcemid treatment and 80 for [3H] thymidine treatment.

Molecular and functional characterization of Toll-like receptor 5 (TLR5) in Aseel and White Leghorn chicken

2017 ◽  
Author(s):  
T. R. Kannaki ◽  
M. R. Reddy ◽  
K. S. Raja ◽  
Ravindra . ◽  
R. N. Chatterjee
Keyword(s):  
Functional Characterization ◽  
Adaptive Immune Response ◽  
Heart Tissue ◽  
Transcript Expression ◽  
White Leghorn ◽  
White Leghorn Chicken ◽  
Significant Difference ◽  
Toll Like Receptor 5 ◽  
Molecular Patterns

Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) which detect pathogen-associated molecular patterns (PAMPs) and initiate the innate as well as adaptive immune response. In the present study TLR5 gene from Aseel and White Leghorn was amplified and sequenced by primer walking method. The sequence analysis revealed that Aseel TLR5 shared 97-98% and 98-99% homology with other chicken breeds at nucleotide and amino acid levels, respectively. Further, the TLR5 mRNA expression levels were quantified in different tissues of day-old Aseel and WL chicks by real time PCR assay. TLR5 mRNA expressions were significantly higher in liver, spleen and intestine of Aseel than White Leghorn chicken (P less than 0.01). However, in bone marrow significantly higher expression was observed in WL than Aseel chicken (P less than 0.01) and no significant difference in transcript expression was found in muscle, bursa and heart tissue. In vitro stimulation of PBMCs of Aseel and WL with recombinant flagellin resulted in significantly higher levels of proinflammatory cytokine IL-1â gene expression in Aseel birds than WL. Polymorphisms in ligand binding region, higher transcript expression in tissues at the site of microbial entry and higher pro-inflammatory response to flagellin stimulation in Aseel chicken better immune competence in this native chicken breed.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation
Sign in / Sign up

Export Citation Format

Share Document