Effect of Ofloxacin on Corneal Epithelial Wound Healing Evaluated by In Vitro and In Vivo Methods

Murine alternatively activated macrophages can exert anti-inflammatory effects. We sought to determine if IL-4–treated human macrophages [i.e., hM(IL4)] would promote epithelial wound repair and can serve as a cell transfer treatment for inflammatory bowel disease (IBD). Blood monocytes from healthy volunteers and patients with active and inactive IBD were converted to hM(IL4)s. IL-4 treatment of blood-derived macrophages from healthy volunteers and patients with inactive IBD resulted in a characteristic CD206+CCL18+CD14low/− phenotype (RNA-seq revealed IL-4 affected expression of 996 genes). Conditioned media from freshly generated or cryopreserved hM(IL4)s promoted epithelial wound healing in part by TGF, and reduced cytokine-driven loss of epithelial barrier function in vitro. Systemic delivery of hM(IL4) to dinitrobenzene sulphonic acid (DNBS)–treated Rag1−/− mice significantly reduced disease. These findings from in vitro and in vivo analyses provide proof-of-concept support for the development of autologous M(IL4) transfer as a cellular immunotherapy for IBD.

Download Full-text

Fibronectin Enhancement of Corneal Epithelial Wound Healing of Rabbits In Vivo

Archives of Ophthalmology ◽

10.1001/archopht.1984.01040030369040 ◽

1984 ◽

Vol 102 (3) ◽

pp. 455-456 ◽

Cited By ~ 76

Author(s):

T. Nishida ◽

S. Nakagawa ◽

C. Nishibayashi ◽

H. Tanaka ◽

R. Manabe

Keyword(s):

Wound Healing ◽

Corneal Epithelial ◽

Epithelial Wound Healing

Download Full-text

Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects

International Journal of Molecular Sciences ◽

10.3390/ijms20071655 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1655 ◽

Cited By ~ 9

Author(s):

Carlota Suárez-Barrio ◽

Jaime Etxebarria ◽

Raquel Hernáez-Moya ◽

Marina del Val-Alonso ◽

Maddalen Rodriguez-Astigarraga ◽

...

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Synergistic Effects ◽

Sodium Hyaluronate ◽

In Vitro Proliferation ◽

Corneal Epithelial ◽

Equivalent Efficacy ◽

And Migration

The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, ß4 integrin, ZO-1, α-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.

Download Full-text