Prevalence of Four Virulence Genes in Campylobacter jejuni Determined by PCR and Sequence Analysis

2005 ◽  
Vol 9 (4) ◽  
pp. 211-215 ◽  
Author(s):  
Vasilios Kordinas ◽  
Chryssoula Nicolaou ◽  
Anastassios Ioannidis ◽  
Eleni Papavasileiou ◽  
Nicolaos John Legakis ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Campylobacter Jejuni ◽  
Virulence Genes

scholarly journals Detection of Campylobacter jejuni, Campylobacter coli, and virulence genes in poultry products marketed in Northeastern Brazil

2021 ◽  
Vol 10 (10) ◽  
pp. e542101019224
Author(s):  
Felipe Pereira de Melo ◽  
Priscila Oliveira da Silva ◽  
Saruanna Millena dos Santos Clemente ◽  
Renata Pimentel Bandeira de Melo ◽  
José Givanildo da Silva ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Virulence Genes ◽  
Northeastern Brazil ◽  
Campylobacter Coli ◽  
Public Markets ◽  
Poultry Products ◽  
Public Market

In this study, we evaluated the prevalence of Campylobacter jejuni, Campylobcater coli, and virulence genes in fresh, chilled, and frozen chicken carcasses with livers and gizzards sold in public markets and supermarkets. Of the 90 samples analyzed, C. jejuni was the most prevalent, with 28.8% of positive samples, whereas C. coli was positive in 15.6% of samples. In public market samples, C. coli had a higher prevalence than C. jejuni, with 16.7% positive samples detected, whereas in supermarket samples, C. jejuni was more prevalent (36.7% positivity). C. jejuni was detected in all forms of commercialized carcasses; however, there was a higher prevalence (43.3%) in chilled samples than C. coli, which was not detected in frozen samples but showed a higher prevalence (16.7%) in fresh samples. Both species were detected in different poultry products, with C. jejuni being more prevalent (53.3%) in liver samples. C. coli showed a higher prevalence in samples of meat pieces (10%). The presence of five virulence genes related to adherence (Peb1, JlpA, CadF, and CapA) and invasion (CiaB) was also observed in both species.

scholarly journals A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection

2007 ◽  
Vol 75 (4) ◽  
pp. 1994-2003 ◽  
Author(s):  
Robert O. Watson ◽  
Veronica Novik ◽  
Dirk Hofreuter ◽  
María Lara-Tejero ◽  
Jorge E. Galán
Keyword(s):  
Campylobacter Jejuni ◽  
Virulence Genes ◽  
Potential Role ◽  
Bacterial Pathogen ◽  
Adaptor Protein ◽  
Myeloid Differentiation ◽  
Deficient Mouse ◽  
Immunocompetent Mice ◽  
Myeloid Differentiation Factor ◽  
Deficient Mice

ABSTRACT Campylobacter jejuni is a major worldwide cause of enteric illnesses. Adult immunocompetent mice are not susceptible to C. jejuni infection. However, we show here that mice deficient in the adaptor protein myeloid differentiation factor 88 (MyD88), which is required for signaling through most Toll-like receptors, can be stably colonized by C. jejuni but not by isogenic derivatives carrying mutations in known virulence genes. We also found that Nramp1 deficiency increases the mouse susceptibility to C. jejuni infection when administered systemically. These results indicate that MyD88-deficient mice could be a useful model to study C. jejuni colonization and reveal a potential role for Nramp1 in the control of this bacterial pathogen.

scholarly journals Prevalence of virulence genes in strains of Campylobacter jejuni isolated from human, bovine and broiler

2013 ◽  
Vol 44 (4) ◽  
pp. 1223-1229 ◽  
Author(s):  
Gisela González-Hein ◽  
Bernardo Huaracán ◽  
Patricia García ◽  
Guillermo Figueroa
Keyword(s):  
Campylobacter Jejuni ◽  
Virulence Genes

scholarly journals Point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of Campylobacter jejuni

2016 ◽  
Vol 113 (38) ◽  
pp. 10690-10695 ◽  
Author(s):  
Zuowei Wu ◽  
Balamurugan Periaswamy ◽  
Orhan Sahin ◽  
Michael Yaeger ◽  
Paul Plummer ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Campylobacter Jejuni ◽  
Outer Membrane Protein ◽  
Animal Health ◽  
Major Outer Membrane Protein ◽  
Whole Genome Sequence ◽  
Bacterial Strains ◽  
Zoonotic Pathogen

Infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. Association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. Campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. C. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of C. jejuni recently emerged, which is able to translocate across the intestinal tract, causing systemic infection and abortion in pregnant animals. The genetic basis responsible for this hypervirulence is unknown. Here, we developed a strategy, termed “directed genome evolution,” by using hybridization between abortifacient and nonabortifacient strains followed by selection in an animal disease model and whole-genome sequence analysis. This strategy successfully identified SNPs in porA, encoding the major outer membrane protein, are responsible for the hypervirulence. Defined mutagenesis verified that these mutations were both necessary and sufficient for causing abortion. Furthermore, sequence analysis identified porA as the gene with the top genome-wide signal of adaptive evolution using Fu’s Fs, a population genetic metric for recent population size changes, which is consistent with the recent expansion of clone “sheep abortion.” These results identify a key virulence factor in Campylobacter and a potential target for the control of this zoonotic pathogen. Furthermore, this study provides general, unbiased experimental and computational approaches that are broadly applicable for efficient elucidation of disease-causing mutations in bacterial pathogens.

Virulence comparison of human and poultry Campylobacter jejuni isolates in a mouse model

2017 ◽  
Vol 5 (10) ◽  
Author(s):  
Darinka Vučković ◽  
Maja Šikić Pogačar ◽  
Peter Raspor ◽  
Maja Abram ◽  
Sonja Smole Možina ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Mouse Liver ◽  
Virulence Genes ◽  
Cytokine Production ◽  
Host Responses ◽  
Human Origin ◽  
Tnf Α ◽  
Food Isolate ◽  
Ifn Γ ◽  
Food Model

 ABSTRACTObjective: Research into Campylobacter jejuni pathogenesis and host responses to C. jejuni infection is needed in the fight against human campylobacteriosis.Methods: We established intravenous infections of BALB/c mice with either a C. jejuni food isolate or C. jejuni of human origin. Further we include PCR to demonstrate the presence and stability of the putative virulence genes cadF, virbB11, cdtB, cdtC, ceuE in C. jejuni isolates and we examined cytokine production of IL-6, IL-12, TNF-α, IFN-γ, IL-10 in the livers of these infected mice.Results: We confirm here the presence of the cadF, cdtB, cdtC and ceuE genes in a food and a clinical C. jejuni isolate, with no sequence changes after the C. jejuni sub-culturing in a food model and when recovered from mouse liver after infection. Both of these C. jejuni isolates persisted in the mouse livers and activated comparable cytokine patterns for IL-12, TNF-α, IFN-γ and IL-10, with down-regulation of IL-6.Conclusions: These data show the comparability of these C. jejuni food and clinical isolates in terms of the prevalence and stability of their putative virulence genes and the outcome of disease during systemic murine campylobacteriosis.

scholarly journals Prevalence of Virulence Genes and Cytolethal Distending Toxin Production in Campylobacter jejuni Isolates from Diarrheal Patients in Bangladesh

2008 ◽  
Vol 46 (4) ◽  
pp. 1485-1488 ◽  
Author(s):  
K. A. Talukder ◽  
M. Aslam ◽  
Z. Islam ◽  
I. J. Azmi ◽  
D. K. Dutta ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Virulence Genes ◽  
Toxin Production ◽  
Cytolethal Distending Toxin

Pathogenesis in lambs and sequence analysis of putative virulence genes of Brazilian orf virus isolates

2014 ◽  
Vol 174 (1-2) ◽  
pp. 69-77 ◽  
Author(s):  
Mathias Martins ◽  
Juliana F. Cargnelutti ◽  
Rudi Weiblen ◽  
Eduardo F. Flores
Keyword(s):  
Sequence Analysis ◽  
Virulence Genes ◽  
Orf Virus ◽  
Virus Isolates
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