Development of target-site resistance (TSR) in Alopecurus myosuroides in Germany between 2004 and 2012

2013 ◽  
Vol 120 (4) ◽  
pp. 179-187 ◽  
Author(s):  
Maria Rosenhauer ◽  
Bernhard Jaser ◽  
Friedrich G. Felsenstein ◽  
Jan Petersen
Keyword(s):  
Target Site ◽  
Alopecurus Myosuroides ◽  
Target Site Resistance

scholarly journals First Detection and Characterization of Cross- and Multiple Resistance to Acetyl-CoA Carboxylase (ACCase)- and Acetolactate Synthase (ALS)-Inhibiting Herbicides in Black-Grass (Alopecurus myosuroides) and Italian Ryegrass (Lolium multiflorum) Populations from Ireland

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1272
Author(s):  
Vijaya Bhaskar Alwarnaidu Vijayarajan ◽  
Patrick D Forristal ◽  
Sarah K Cook ◽  
David Schilder ◽  
Jimmy Staples ◽  
...  
Keyword(s):  
Lolium Multiflorum ◽  
Acetolactate Synthase ◽  
Target Site ◽  
Italian Ryegrass ◽  
Integrated Weed Management ◽  
Multiple Resistance ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Alopecurus Myosuroides ◽  
Target Site Resistance

Understanding the resistance spectrum and underlying genetic mechanisms is critical for managing herbicide-resistant populations. In this study, resistance to acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors was investigated in four suspected resistant populations of Alopecurus myosuroides (ALOMY-001 to ALOMY-004) and Lolium multiflorum (LOLMU-001 to LOLMU-004), collected from cereal production fields in Ireland. Glasshouse assays with three ALOMY-active herbicides [propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron (ALS)] or five LOLMU-active herbicides [pinoxaden, propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron, pyroxsulam (ALS)], and target-site resistance mechanism studies, based on pyrosequencing, were carried out in each of those populations. For A. myosuroides, Ile-1781-Leu ACCase mutation contributed to propaquizafop and cycloxydim resistance (shoot dry weight GR50 resistance factor (RF) = 7.5–35.5) in all ALOMY populations, and the independent Pro-197-Thr or Pro-197-Ser ALS mutation contributed to mesosulfuron + iodosulfuron resistance (RF = 3.6–6.6), in ALOMY-002 to ALOMY-004. Most of the analyzed plants for these mutations were homo/heterozygous combinations or only heterozygous. For L. multiflorum, phenotypic resistance to mesosulfuron + iodosulfuron (RF = 11.9–14.6) and pyroxsulam (RF = 2.3–3.1) was noted in all LOLMU populations, but the Pro-197-Gln or Pro-197-Leu ALS mutation (mostly in homozygous status) was identified in LOLMU-001, LOLMU-002 and LOLMU-004 only. Additionally, despite no known ACCase mutations in any LOLMU populations, LOLMU-002 survived pinoxaden and propaquizafop application (RF = 3.4 or 1.3), and LOLMU-003 survived pinoxaden (RF = 2.3), suggesting the possibility of non-target-site resistance mechanisms for ACCase and/or ALS resistance in these populations. Different resistance levels, as evidenced by a reduction in growth as dose increased above field rates in ALOMY and LOLMU, were due to variations in mutation rate and the level of heterozygosity, resulting in an overall resistance rating of low to moderate. This is the first study confirming cross- and multiple resistance to ACCase- and ALS-inhibiting herbicides, highlighting that resistance monitoring in A. myosuroides and L. multiflorum in Ireland is critical, and the adoption of integrated weed management strategies (chemical and non-chemical/cultural strategies) is essential.

scholarly journals Molecular Basis of Blackgrass (Alopecurus Myosuroides HUDS.) Resistance to Sulfonylurea Herbicides

2011 ◽  
Vol 51 (2) ◽  
pp. 130-133 ◽  
Author(s):  
Michał Krysiak ◽  
Stanisław Gawroński ◽  
Roman Kierzek ◽  
Kazimierz Adamczewski
Keyword(s):  
Molecular Analysis ◽  
Molecular Basis ◽  
Acetolactate Synthase ◽  
Target Site ◽  
Sulfonylurea Herbicides ◽  
Resistant Plant ◽  
Alopecurus Myosuroides ◽  
Mechanism Of Resistance ◽  
Herbicide Treatment ◽  
Target Site Resistance

Molecular Basis of Blackgrass (Alopecurus MyosuroidesHUDS.) Resistance to Sulfonylurea HerbicidesBlackgrass biotype resistant to the mesosulfuron + iodosulfuron mixture has been found in Poland and was investigated in this study. Seedlings that survived double-dosed herbicide treatment were submitted to molecular analysis in order to explain the mechanism of resistance. Domains A and B of the acetolactate synthase gene were amplified by PCR and then sequenced. Biotypes which were both resistant and susceptible to mesosulfuron+iodosulfuron were analyzed. The comparison of the obtained sequences was made on a nucleotide and aminoacid level. The comparison revealed a substitution of proline codon to histidine codon in position 197 in each resistant plant. Mutation Pro197His is the basis of the target site resistance ofA. myosuroidesto sulfonylureas. There were no other mutations inalsgene of the biotype that might modify the level of resistance to these herbicides.

A pollen test to detect ACCase target-site resistance within Alopecurus myosuroides populations

Weed Research ◽  
2008 ◽  
Vol 40 (2) ◽  
pp. 151-162 ◽  
Author(s):  
Letouzé ◽  
Gasquez
Keyword(s):  
Target Site ◽  
Alopecurus Myosuroides ◽  
Target Site Resistance

Characterisation of target-site resistance to ACCase-inhibiting herbicides in the weedAlopecurus myosuroides (black-grass)

2003 ◽  
Vol 59 (2) ◽  
pp. 190-201 ◽  
Author(s):  
Stephen R Moss ◽  
Kay M Cocker ◽  
Amanda C Brown ◽  
Linda Hall ◽  
Linda M Field
Keyword(s):  
Target Site ◽  
Target Site Resistance

Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields

2021 ◽  
Author(s):  
Norazua Zakaria ◽  
Rabiatuladawiyah Ruzmi ◽  
Salmah Moosa ◽  
Norhayu Asib ◽  
Dzarifah Zulperi ◽  
...  
Keyword(s):  
Rice Fields ◽  
Target Site ◽  
Invasive Weed ◽  
Target Site Resistance

The target-site based resistance mechanism of Alopecurus myosuroides Huds. to pyroxsulam

2021 ◽  
pp. 105707
Author(s):  
Zhaofeng Huang ◽  
Xingtao Lu ◽  
Shouhui Wei ◽  
Chaoxian Zhang ◽  
Cuilan Jiang ◽  
...  
Keyword(s):  
Resistance Mechanism ◽  
Target Site ◽  
Alopecurus Myosuroides

scholarly journals Pollen Expression of Herbicide Target Site Resistance Genes in Annual Ryegrass (Lolium rigidum)

1993 ◽  
Vol 102 (3) ◽  
pp. 1037-1041 ◽  
Author(s):  
J. Richter ◽  
S. B. Powles
Keyword(s):  
Resistance Genes ◽  
Target Site ◽  
Annual Ryegrass ◽  
Lolium Rigidum ◽  
Target Site Resistance

Controlling Herbicide-Resistant Annual Bluegrass (Poa annua) Phenotypes with Methiozolin

2017 ◽  
Vol 31 (3) ◽  
pp. 470-476 ◽  
Author(s):  
James T. Brosnan ◽  
Jose J. Vargas ◽  
Gregory K. Breeden ◽  
Sarah L. Boggess ◽  
Margaret A. Staton ◽  
...  
Keyword(s):  
Acetolactate Synthase ◽  
Target Site ◽  
Multiple Resistance ◽  
Herbicide Resistant ◽  
Annual Bluegrass ◽  
Microtubule Inhibitors ◽  
Effective Option ◽  
Target Site Resistance ◽  
Susceptible Control ◽  
Als Inhibitors

Methiozolin is an isoxazoline herbicide being investigated for selective POST annual bluegrass control in managed turfgrass. Research was conducted to evaluate methiozolin efficacy for controlling two annual bluegrass phenotypes with target-site resistance to photosystem II (PSII) or enolpyruvylshikimate-3-phosphate synthase (EPSPS)-inhibiting herbicides (i.e., glyphosate), as well as phenotypes with multiple resistance to microtubule and EPSPS or PSII and acetolactate synthase (ALS)-inhibiting herbicides. All resistant phenotypes were established in glasshouse culture along with a known herbicide-susceptible control and treated with methiozolin at 0, 125, 250, 500, 1000, 2000, 4000, or 8000 g ai ha−1. Methiozolin effectively controlled annual bluegrass with target-site resistance to inhibitors of EPSPS, PSII, as well as multiple resistance to EPSPS and microtubule inhibitors. Methiozolin rates required to reduce aboveground biomass of these resistant phenotypes 50% (GR50 values) were not significantly different from the susceptible control, ranging from 159 to 421 g ha−1. A phenotype with target-site resistance to PSII and ALS inhibitors was less sensitive to methiozolin (GR50=862 g ha−1) than a susceptible phenotype (GR50=423 g ha−1). Our findings indicate that methiozolin is an effective option for controlling select annual bluegrass phenotypes with target-site resistance to several herbicides.

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