Maize Voc Induction after Infection by the Bacterial Pathogen, Pantoea ananatis, Alters Neighbouring Plant Voc Emissions

Wheat (Triticum aestivum) is one of the most economically important crops in the world. During the routine monitoring of wheat pest, the cereal leaf beetle (CLB, Oulema melanopus, Coleoptera, Chrysomelidae), in the Greater Poland region, it was observed that some leaves wounded by CLB also displayed brownish lesions with clear margins and yellow halo, disease symptoms resembling a bacterial infection. The aim of this study was therefore to investigate those symptoms to establish a causal agent of the disease. The identification based on the results of the Biolog’s Gen III system, 16S rRNA, and gyrB genes sequencing, revealed the presence of eight strains of Pantoea ananatis bacteria. Four strains were derived from wheat leaves (Ta024, Ta027, Ta030, Ta046), and four from the CLB’s oral secretion (OUC1, OUD2, OUF2, and OUG1). They shared the nucleotide identity ranging from 99 to 100% to P. ananatis strains deposited in the GenBank database. Additionally, the multi-locus sequence analysis (MLSA) of concatenated sequences of partial atpD, fusA, gyrB, rplB, and rpoB genes was performed. All P. ananatis strains isolated in Poland, grouped into one cluster supported with high bootstrap value. Pathogenicity tests performed on four varieties of wheat plants have identified P. ananatis strains as a causal agent of wheat disease. To our knowledge, this is the first report of P. ananatis affecting wheat plants.

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Characterization of Pantoea ananatis Isolated from Garlic and Shallot

Jurnal Perlindungan Tanaman Indonesia ◽

10.22146/jpti.27407 ◽

2018 ◽

Vol 21 (2) ◽

pp. 120

Author(s):

Nanik Nurjanah ◽

Tri Joko ◽

Siti Subandiyah

Keyword(s):

Allium Sativum ◽

Bacterial Pathogen ◽

Leaf Blight ◽

Gyrb Gene ◽

Phenotypic Characterization ◽

Pathogenicity Test ◽

Pantoea Ananatis ◽

Gram Negative ◽

Central Java

The new disease on garlic (Allium sativum) and shallot (A. cepa L. aggregatum group) have been found in several production centers of garlic and shallot in Tawangmangu and Temanggung, Central Java. The infected plants showed symptoms of leaf blight accompanied by chlorosis. The objective of this study was to determine the pathogen that causes leaf blight and chlorosis based on the phenotypic characterization and gyrB gene sequences analysis. The research started from the isolation of pathogen, physiological and biochemical test, DNA extraction, and sequence analysis of gyrB using gyrB 01-F and gyrB 02-R primer. The results showed that the isolated bacterial pathogen have a yellow pigment, slimy colonies with regular borders, convex, gram-negative, non-spore, facultative anaerobic, motile, catalase production, indole production, and acid production from D-glucose, D-mannitol, sucrose, and lactose. From the pathogenicity test, it was found that the bacteria produced the typical symptom of leaf blight. Characterization of pathogens based on gyrB gene sequence revealed that the pathogen was placed in the group of Pantoea ananatis. IntisariPenyakit baru pada bawang putih (Allium sativum) dan bawang merah (A. cepa L. aggregatum group) telah ditemukan di beberapa sentra produksi bawang putih dan bawang merah di Tawangmangu dan Temanggung, Jawa Tengah. Tanaman yang terinfeksi menunjukkan gejala hawar daun disertai klorosis. Tujuan penelitian untuk mengetahui karakter patogen berdasarkan fenotipik dan sekuen gen gyrB. Penelitian dimulai dengan isolasi bagian tanaman yang sakit, uji fisiologi dan biokimia, ekstraksi DNA dengan metode CTAB/NaCl dan amplifikasi gen gyrB menggunakan primer gyrB 01-F and gyrB 02-R. Hasil uji menunjukkan koloni berlendir, cembung, pigmen berwarna kuning, gram negative, tidak berspora, aerob fakultatif, motil, produksi katalase, indol, membentuk asam dari D-glukosa, D-monnitol, sukrosa dan laktosa, dan patogenesitas positif. Karakterisasi patogen berdasarkan sekuen gen gyrB, menunjukkan patogen hawar daun berkerabat dekat dengan Pantoea ananatis.

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Exemplar Abstract for Legionella pneumophila Brenner et al. 1979 (Approved Lists 1980) emend. Brenner et al. 1988, Pantoea ananatis corrig. (Serrano 1928) Mergaert et al. 1993 and Pantoea ananas (sic) (Serrano 1928) Mergaert et al. 1993.

The NamesforLife Abstracts ◽

10.1601/ex.20403 ◽

2003 ◽

Author(s):

Charles Thomas Parker ◽

Dorothea Taylor ◽

George M Garrity

Keyword(s):

Legionella Pneumophila ◽

Pantoea Ananatis

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Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

Journal of Clinical and Health Sciences ◽

10.24191/jchs.v2i1.5861 ◽

2016 ◽

Vol 1 (2) ◽

pp. 22 ◽

Cited By ~ 2

Author(s):

Navindra Kumari Palanisamy ◽

Parasakthi Navaratnam ◽

Shamala Devi Sekaran

Keyword(s):

Antibiotic Resistance ◽

Streptococcus Pneumoniae ◽

Resistance Genes ◽

Bacterial Pathogen ◽

Antibiotic Resistance Genes ◽

Pcr Amplification ◽

Penicillin Resistance ◽

Penicillin Binding Proteins ◽

Efflux Mechanism ◽

Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

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