Pseudomonas syringae pv. solidagae pv. nov., the Causal Agent of Bacterial Leaf Spot of Tall Goldenrod Solidago altissima L.

2001 ◽  
Vol 67 (4) ◽  
pp. 303-308 ◽  
Author(s):  
Mamoru SATO ◽  
Kenji WATANABE ◽  
Yoko SATO
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Causal Agent ◽  
Solidago Altissima ◽  
Bacterial Leaf Spot ◽  
Tall Goldenrod

scholarly journals Multiple resistance to bacterial halo blight and bacterial leaf spot in Coffea spp.*

2019 ◽  
Vol 86 ◽  
Author(s):  
Lucas Mateus Rivero Rodrigues ◽  
Suzete Aparecida Lanza Destéfano ◽  
Irene Maria Gatti de Almeida ◽  
Luís Otávio Saggion Beriam ◽  
Masako Toma Braghini ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Low Cost ◽  
Durable Resistance ◽  
Genetic Resistance ◽  
Causal Agent ◽  
Limiting Factor ◽  
Bacterial Leaf Spot ◽  
Halo Blight ◽  
Moisture Conditions

ABSTRACT Breeding for genetic resistance is an important method of crop disease management, due to the numerous benefits and low cost of establishment. In this study, progenies of 11 Coffea species and 16 wild C. arabica accessions were tested for their response to Pseudomonas syringae pv. garcae, the causal agent of bacterial halo blight, a widespread disease in the main coffee-producing regions of Brazil and considered a limiting factor for cultivation in pathogen-favorable areas; and also to P. syringae pv. tabaci, causal agent of bacterial leaf spot, a highly aggressive disease recently detected in Brazil. Separate experiments for each disease were carried out in a greenhouse, with artificial pathogen inoculations and ideal moisture conditions for disease development. The results showed that C. canephora, C. congensis, C. eugenioides, C. stenophylla, and C. salvatrix progenies, the wild C. arabica accessions Dilla & Alghe and Palido Viridis, and cultivar IPR 102 contain satisfactory levels of simultaneous resistance against bacterial halo blight and bacterial leaf spot. These results are useful in breeding programs for durable resistance to multiple biotic agents, providing new combinations of resistance alleles by hybridization, as well as for phytopathological studies, to identify infraspecific variability of the pathogens.

scholarly journals Pseudomonas syringae pv. spinaciae pv.nov., the Causal Agent of Bacterial Leaf Spot of Spinach in Japan.

1998 ◽  
Vol 64 (4) ◽  
pp. 264-269 ◽  
Author(s):  
Katsumi OZAKI ◽  
Toshihiko KIMURA ◽  
Kunihiko MATSUMOTO
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Causal Agent ◽  
Bacterial Leaf Spot

Pseudomonas syringae pv. alliifistulosi pv. nov., the causal agent of bacterial leaf spot of onions

2018 ◽  
Vol 84 (5) ◽  
pp. 343-358
Author(s):  
Mizue Tsuji ◽  
Yuichi Takikawa
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Causal Agent ◽  
Bacterial Leaf Spot

First report of bacterial leaf spot on Chenopodium quinoa caused by Pseudomonas syringae in Colombia

2021 ◽  
Author(s):  
I. Fonseca-Guerra ◽  
C. Chiquillo ◽  
M. J. Padilla ◽  
M. Benavides-Rozo
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Chenopodium Quinoa ◽  
Bacterial Leaf Spot ◽  
First Report

scholarly journals Epidemiology and management of bacterial leaf spot on watermelon caused byPseudomonas syringae

Plant Disease ◽  
2017 ◽  
Vol 101 (7) ◽  
pp. 1222-1229 ◽  
Author(s):  
E. A. Newberry ◽  
L. Ritchie ◽  
B. Babu ◽  
T. Sanchez ◽  
K. A. Beckham ◽  
...  
Keyword(s):  
Disease Severity ◽  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Negative Relationship ◽  
Disease Outbreaks ◽  
Field Trials ◽  
Multiple Regression Model ◽  
Bacterial Leaf Spot ◽  
Progress Curve ◽  
Cool Conditions

Bacterial leaf spot of watermelon caused by Pseudomonas syringae has been an emerging disease in the southeastern United States in recent years. Disease outbreaks in Florida were widespread from 2013 to 2014 and resulted in foliar blighting at the early stages of the crop and transplant losses. We conducted a series of field trials at two locations over the course of two years to examine the chemical control options that may be effective in management of this disease, and to investigate the environmental conditions conducive for bacterial leaf spot development. Weekly applications of acibenzolar-S-methyl (ASM) foliar, ASM drip, or copper hydroxide mixed with ethylene bis-dithiocarbamate were effective in reducing the standardized area under the disease progress curve (P < 0.05). Pearson’s correlation test demonstrated a negative relationship between the average weekly temperature and disease severity (–0.77, P = 0.0002). When incorporated into a multiple regression model with the square root transformed average weekly rainfall, these two variables accounted for 71% of the variability observed in the weekly disease severity (P < 0.0001). This information should be considered when choosing the planting date for watermelon seedlings as the cool conditions often encountered early in the spring season are conducive for bacterial leaf spot development.

scholarly journals Bacterial Leaf Spot of Celery in California: Etiology, Epidemiology, and Role of Contaminated Seed

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 892-896 ◽  
Author(s):  
E. L. Little ◽  
S. T. Koike ◽  
R. L. Gilbertson
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Field Tests ◽  
Hot Water ◽  
Disease Development ◽  
Bacterial Leaf Spot ◽  
Overhead Irrigation ◽  
Celery Seed ◽  
Salinas Valley

Pseudomonas syringae pv. apii, causal agent of bacterial leaf spot (BLS) of celery, was first identified in California in 1989. By 1991, BLS was apparent in all celery-growing areas of the state. Greenhouse-produced transplants were affected most severely, and disease incidence approached 100% in some greenhouses. In this study, sources of inoculum and factors contributing to disease development were investigated in three Salinas Valley greenhouse operations during the 1991, 1992, and 1993 celery transplant seasons (January to August). Epiphytic P. syringae pv. apii was not detected on celery transplants until April or May of each year. Increased epiphytic populations preceded BLS outbreaks, and high-pressure, overhead irrigation favored bacterial infiltration and disease development. In seed-wash assays, P. syringae pv. apii was recovered from 5 of 24 commercial celery seed lots. In field tests, epiphytic P. syringae pv. apii was found on umbels of inoculated celery plants, and seeds from these plants were heavily contaminated with P. syringae pv. apii. Contaminated seed produced seedlings with large epiphytic P. syringae pv. apii populations. Hot-water treatment (50°C for 25 min) eliminated >99.9% of seed contamination. Based on these results, disease management techniques are proposed.

scholarly journals First Report of Pseudomonas syringae pv. aptata Causing Bacterial Leaf Spot on Sugar Beet in Serbia

Plant Disease ◽  
2015 ◽  
Vol 99 (2) ◽  
pp. 281-281 ◽  
Author(s):  
V. Stojšin ◽  
J. Balaž ◽  
D. Budakov ◽  
Slaviša Stanković ◽  
I. Nikolić ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Leaf Surface ◽  
Negative Control ◽  
Gyrb Gene ◽  
Bacterial Suspension ◽  
Bacterial Strains ◽  
Gene Sequences ◽  
Bacterial Leaf Spot

A severe bacterial leaf spot was observed during June and July 2013 on commercial cultivars of sugar beet (Beta vulgaris var. saccharifera) in the Vojvodina Province of Serbia. Serbia is a major sugar beet production area in southeastern Europe, with 62,895 ha and 3 million tons of sugar beet yield in 2013. A foliar leaf spot observed in 25 commercial sugar beet fields surveyed ranged from 0.1 to 40% severity. Symptoms were characterized as circular or irregular, 5- to 20-mm diameter, white to light brown necrotic spots, each with a dark margin. Diseased leaves were rinsed in sterilized, distilled water (SDW) and dried at room temperature, and leaf sections taken from the margin of necrotic tissue were macerated in SDW. Isolations from 48 symptomatic leaves onto nutrient agar with 5% (w/v) sucrose (NAS) produced bacterial colonies that were whitish, circular, dome-shaped, and Levan-positive. Representative isolates (n = 105) were Gram negative; aerobic; positive for catalase, fluorescence on King's medium B, and tobacco hypersensitivity; and negative for oxidase, potato rot, and arginine dehydrolase. These reactions corresponded to LOPAT group Ia, which includes Pseudomonas syringae pathovars (2). Repetitive extragenic palindromic sequence (rep)-PCR was used for genetic fingerprinting the isolates using the REP, ERIC, and BOX primers. Twenty-five different profiles were obtained among the strains. From each profile group, one representative strain was sequenced for the gyrB gene (1). Four heterogenic groups were observed, and representative gyrB gene sequences of each group were deposited in the NCBI GenBank (Accession Nos. KJ950024 to KJ950027). The sequences were compared with those of pathotype strain P. syringae pv. aptata CFBP 1617 deposited in the PAMDB database; one strain was 100% homologous, and the other three were 99% homologous. To fulfill identification of the Serbian sugar beet isolates, gltA and rpoD partial gene sequences were determined (1), and the sequences were deposited as Accession Nos. KM386838 to KM386841 for gltA and KM386830 to KM38683033 for rpoD. The sequences were 100% homologous with those of pathotype strain CFBP 1617. Pathogenicity of each of four representative bacterial strains was tested on 3-week-old plants of the sugar beet cultivars Marinela, Serenada, and Jasmina (KWS, Belgrade, Serbia) and Lara (NS Seme, Novi Sad, Serbia) by atomizing a bacterial suspension of ~106 CFU/ml of the appropriate isolate onto the abaxial leaf surface of three plants per cultivar until water-soaking of the leaf surface was observed. Three plants of each cultivar atomized similarly with P. syringae pv. aptata CFBP 2473 and SDW served as positive and negative control treatments, respectively. Inoculated plants were kept in a clear plastic box at 80 to 100% RH and 17 ± 1°C and examined for symptom development over 3 weeks. For all test isolates and the control strain, inoculated leaves first developed water-soaked lesions 7 days after inoculation (DAI). By 10 to 14 DAI, lesions were necrotic and infection had spread to the petioles. By 21 DAI, wilting was observed on more than 50% of inoculated plants. Negative control plants were symptomless. Bacteria re-isolated onto NAS from inoculated leaves had the same colony morphology, LOPAT results, and gyrB partial gene sequences as described for the test strains. No bacteria were re-isolated from negative control plants. Based on these tests, the pathogen causing leaf spot on sugar beet in Serbia was identified as P. syringae pv. aptata. References: (1) P. Ferrente and M. Scortichini. Plant Pathol. 59:954, 2010. (2) R. A. Lelliott et al. J. Appl. Bacteriol. 29:470, 1966.

scholarly journals Complete and Draft Genome Sequences of the Cruciferous Pathogens Pseudomonas cannabina pv. alisalensis and Pseudomonas syringae pv. maculicola

2021 ◽  
Vol 10 (17) ◽  
Author(s):  
Takashi Fujikawa ◽  
Yuichi Takikawa ◽  
Yasuhiro Inoue
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Draft Genome ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Genome Sequences ◽  
Bacterial Leaf Spot ◽  
Content Type

ABSTRACT Pseudomonas cannabina pv. alisalensis and Pseudomonas syringae pv. maculicola cause bacterial leaf blight and bacterial leaf spot of crucifers (Brassicaceae). Both pathogens are threats to the cultivation of cruciferous crops. Here, we sequenced two strains of each pathogen, which will contribute to the development of countermeasures for the above diseases.

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