Irreversible impact of early thermal conditions: an integrative study of developmental plasticity linked to mobility in a butterfly species

Within populations, phenotypic plasticity may allow adaptive phenotypic variation in response to selection generated by environmental heterogeneity. For instance, in multivoltine species, seasonal changes between and within generations may trigger morphological and physiological variation enhancing fitness under different environmental conditions. These seasonal changes may irreversibly affect adult phenotypes when experienced during development. Yet, the irreversible effects of developmental plasticity on adult morphology have rarely been linked to life-history traits even though they may affect different fitness components such as reproduction, mobility and self-maintenance. To address this issue, we raised larvae of Pieris napi butterflies under warm or cool conditions to subsequently compare adult performance in terms of reproduction performance (as assessed through fecundity), displacement capacity (as assessed through flight propensity and endurance) and self-maintenance (as assessed through the measurement of oxidative markers). As expected in ectotherms, individuals developed faster under warm conditions and were smaller than individuals developing under cool conditions. They also had more slender wings and showed a higher wing surface ratio. These morphological differences were associated with changes in the reproductive and flight performances of adults, as individuals developing under warm conditions laid fewer eggs and flew larger distances. Accordingly, the examination of their oxidative status suggested that individuals developing under warm conditions invested more strongly into self-maintenance than individuals developing under cool conditions (possibly at the expense of reproduction). Overall, our results indicate that developmental conditions have long-term consequences on several adult traits in butterflies. This plasticity likely acts on life history strategies for each generation to keep pace with seasonal variations and may facilitate acclimation processes in the context of climate change.

Disentangling environmental drivers of circadian metabolism in desert-adapted mice

ABSTRACT Metabolism is a complex phenotype shaped by natural environmental rhythms, as well as behavioral, morphological and physiological adaptations. Metabolism has been historically studied under constant environmental conditions, but new methods of continuous metabolic phenotyping now offer a window into organismal responses to dynamic environments, and enable identification of abiotic controls and the timing of physiological responses relative to environmental change. We used indirect calorimetry to characterize metabolic phenotypes of the desert-adapted cactus mouse (Peromyscus eremicus) in response to variable environmental conditions that mimic their native environment versus those recorded under constant warm and constant cool conditions, with a constant photoperiod and full access to resources. We found significant sexual dimorphism, with males being more prone to dehydration than females. Under circadian environmental variation, most metabolic shifts occurred prior to physical environmental change and the timing was disrupted under both constant treatments. The ratio of CO2 produced to O2 consumed (the respiratory quotient) reached greater than 1.0 only during the light phase under diurnally variable conditions, a pattern that strongly suggests that lipogenesis contributes to the production of energy and endogenous water. Our results are consistent with historical descriptions of circadian torpor in this species (torpid by day, active by night), but reject the hypothesis that torpor is initiated by food restriction or negative water balance.

Lysathia CTs, LTs, DDM

The CTLs, LLTs and DDM were determined for <i>Lysathia </i>n. sp. in South Africa. Results indicated that the species can tolerate exposure to sub-zero temperatures before death, complete multiple generations per year at sites around South Africa and that it develops quickly in cool conditions. All of these data explain why the agent was so successful at controlling its target weed throughout South Africa and suggest that it could be an agent to consider for release in other cold invaded sites on the American and European continents.

Lysathia CTs, LTs, DDM

The CTLs, LLTs and DDM were determined for <i>Lysathia </i>n. sp. in South Africa. Results indicated that the species can tolerate exposure to sub-zero temperatures before death, complete multiple generations per year at sites around South Africa and that it develops quickly in cool conditions. All of these data explain why the agent was so successful at controlling its target weed throughout South Africa and suggest that it could be an agent to consider for release in other cold invaded sites on the American and European continents.

Potentially Bioaccessible Phenolic and Antioxidant Potential of Fresh and Stored Lentil Sprouts—Effect of Lactobacillus plantarum 299v Enrichment

The phenolic and antioxidant potential of potentially bioaccessible fractions of lentil sprouts was studied. Sprouts were cocultivated with a probiotic to obtain a new functional product and further stored in cool conditions. The fraction obtained after buffer extraction and gastric digestion had higher content of phenolics compared to the control (by 20% and 46%, respectively); however, a 9% decrease was observed in samples obtained after gastrointestinal digestion. After gastrointestinal digestion, the highest content of phenolics (278 µg/g d.w.) was determined in the fresh control sprouts. Compounds neutralizing ABTS and hydroxyl radicals, chelating metal ions, and exhibiting strong reducing power were effectively released after gastrointestinal digestion (e.g., the values of the gastrointestinal digestibility index for chelating power and ability to quench hydroxyl radicals significantly exceeded 1 in all studied samples). It was proved that the enrichment of sprouts with a probiotic and further storage significantly improved the antioxidant potential; compared to the fresh control sprouts, an increase by 45% and 10% was determined after the gastric and gastrointestinal digestion, respectively. Lentil sprouts enriched with L. plantarum 299v may be a new functional product characterized by the high antioxidant capacity of the potentially bioaccessible fraction.

The effect of low-temperature annealing on discordance of U–Pb zircon ages

Discordant U–Pb data of zircon are commonly attributed to Pb loss from domains with variable degree of radiation damage that resulted from α-decay of U and Th, which often complicates the correct age interpretation of the sample. Here we present U–Pb zircon data from 23 samples of ca. 1.7–1.9 Ga granitoid rocks in and around the Siljan impact structure in central Sweden. Our results show that zircon from rocks within the structure that form an uplifted central plateau lost significantly less radiogenic Pb compared to zircon grains in rocks outside the plateau. We hypothesize that zircon in rocks within the central plateau remained crystalline through continuous annealing of crystal structure damages induced from decay of U and Th until uplifted to the surface by the impact event ca. 380 Ma ago. In contrast, zircon grains distal to the impact have accumulated radiation damage at shallow and cool conditions since at least 1.26 Ga, making them vulnerable to fluid-induced Pb-loss. Our data are consistent with studies on alpha recoil and fission tracks, showing that annealing in zircon occurs at temperatures as low as 200–250 °C. Zircon grains from these samples are texturally simple, i.e., neither xenocrysts nor metamorphic overgrowths have been observed. Therefore, the lower intercepts obtained from regression of variably discordant zircon data are more likely recording the age of fluid-assisted Pb-loss from radiation-damaged zircon at shallow levels rather than linked to regional magmatic or tectonic events.

Absolute Dating of the Virje and Hlebine Sites

In this chapter the results of AMS radiocarbon dating of 26 charcoal samples from four sites are discussed. The primary aim was to explore the scope of (dis)continuity of temporally and functionally interconnected types of sites (settlement and iron production workshop). A Bayesian model was created based on the acquired data from excavated sites with three sequences in the model determined. The results show great accordance with the archaeologically based data, with some exceptions that are most probably a result of modelling strategy used. Contemporaneity of all four sites is mostly attested with a possible and very short temporal hiatus around AD 600. Occupation of workshop and residential areas at that time or slightly after may have been linked to the globally attested environmental change during the Bond 1 event when dry and cool conditions prevailed and which could have had an impact on bog iron formation processes.

Limiting Rise in Heat Load With an Ice Vest During Elite Female Rugby Sevens Warm-Ups

Purpose: To determine the effect of wearing a phase-change cooling vest in elite female rugby sevens athletes during (1) a simulated match-day warm-up in hot conditions prior to a training session and (2) a prematch warm-up during a tournament in cool conditions. Methods: This study consisted of 2 randomized independent group designs (separated by 16 d) where athletes completed the same 23- to 25-minute match-day warm-up (1) in hot conditions (range = 28.0°C to 35.1°C wet bulb globe temperature [WBGT]) prior to training and (2) in cool conditions (range = 18.8°C to 20.1°C WBGT) prior to a World Rugby Women’s Sevens Series match. In both conditions, athletes were randomly assigned to wearing either (1) the standardized training/playing ensemble (synthetic rugby shorts and training tee/jersey) or (2) the standardized training/playing ensemble plus a commercial phase-change athletic cooling vest. Group-wise differences in core temperature rise from baseline, global positioning system–measured external locomotive output, and perceptual thermal load were compared. Results: Core temperature rise during a match warm-up was lower in the hot condition only (−0.65°C [95% confidence interval = −1.22°C to −0.08°C], [95% confidence interval = .00 to .51], P = .028). No differences in various external-load variables were observed. Conclusions: Phase-change cooling vests can be worn by athletes prior to, and during, a prematch warm-up in hot conditions to limit excess core temperature rise without adverse effects on thermal perceptions or external locomotion output.

Exercise and Heat Stress: Inflammation and the Iron Regulatory Response

This study determined the impact of heat stress on postexercise inflammation and hepcidin levels. Twelve moderately trained males completed three, 60-min treadmill running sessions under different conditions: (a) COOL, 18 °C with speed maintained at 80% maximum heart rate; (b) HOTHR, 35 °C with speed maintained at 80% maximum heart rate; and (c) HOTPACE, 35 °C completed at the average running speed from the COOL trial. Venous blood samples were collected pre-, post-, and 3-hr postexercise and analyzed for serum ferritin, interleukin-6 (IL-6), and hepcidin concentrations. Average HR was highest during HOTPACE compared with HOTHR and COOL (p < .001). Running speed was slowest in HOTHR compared with COOL and HOTPACE (p < .001). The postexercise increase in IL-6 was greatest during HOTPACE (295%; p = .003). No differences in the IL-6 response immediately postexercise between COOL (115%) and HOTHR (116%) were evident (p = .992). No differences in hepcidin concentrations between the three trials were evident at 3 hr postexercise (p = .407). Findings from this study suggest the IL-6 response to exercise is greatest in hot compared with cool conditions when the absolute running speed was matched. No differences in IL-6 between hot and cool conditions were evident when HR was matched, suggesting the increased physiological strain induced from training at higher intensities in hot environments, rather than the heat per se, is likely responsible for this elevated response. Environmental temperature had no impact on hepcidin levels, indicating that exercising in hot conditions is unlikely to further impact transient alterations in iron regulation, beyond that expected in temperate conditions.

Evaluation of essential oil isolated from dry coriander seeds and recycling of the plant waste under different storage conditions

Background This study focused on the comparison of the essential oil percentage and constituents obtained from the dry seeds and the waste of coriander plant in order to reach the best ways to store the essential oil. Results The chemical composition of coriander oil was analyzed by gas chromatography–mass spectrometry (GC–MS). The analysis of coriander essential oil showed that linalool was the main constituent of dry seed oil under all conditions. It recorded 59.6, 59.28 and 47.69% of the treatments of the oil at zero time, stored in cool conditions and stored at room temperature, respectively. Concerning oil constituents of coriander waste (the remained herb after collecting seeds), the results showed that trans-anethole was the major oil constituent, followed by linalool compound. The quality of the stored oil in the refrigerator after harvest was better than the stored oil from seeds or waste under room temperature. Conclusion The changes have been observed in the chemical composition of coriander oil extracted from seed and waste subjected to different storage conditions. The waste of coriander can be considered a new source of essential oil.