Physiological Races and Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae Isolated from Crisphead Lettuce in Japan

2002 ◽  
Vol 68 (4) ◽  
pp. 292-299 ◽  
Author(s):  
Hideki OGISO ◽  
Masashi FUJINAGA ◽  
Hideki SAITO ◽  
Toshiaki TAKEHARA ◽  
Shigeru YAMANAKA
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups ◽  
Physiological Races

Physiological races and vegetative compatibility groups of butterhead lettuce isolates of Fusarium oxysporum f. sp. lactucae in Japan

2004 ◽  
Vol 70 (6) ◽  
pp. 308-313 ◽  
Author(s):  
Norihito Yamauchi ◽  
Jyuichi Shimazu ◽  
Mamoru Satou ◽  
Seizo Horiuchi ◽  
Takashi Shirakawa
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups ◽  
Physiological Races ◽  
Butterhead Lettuce

scholarly journals Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Aoumria Merzoug ◽  
Lakhdar Belabid
Keyword(s):  
Genetic Structure ◽  
Pisum Sativum ◽  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Susceptible Cultivar ◽  
Vegetative Compatibility Groups ◽  
Physiological Races ◽  
Race 1 ◽  
Western Algeria ◽  
Different Parts

Abstract Fusarium oxysporum f. sp. pisi (FOP) is a significant and destructive pathogen of field pea in Algeria. In the present study, 50 isolates of F. oxysporum f. sp. pisi, the causal agent of pea (Pisum sativum) wilt, collected from different parts of western Algeria and representing four races of the pathogen, were analyzed for virulence. The wilt incidence ranged from 6.66 to 88.33% on a highly susceptible cultivar (Little Marvel). Twenty-one isolates belonging to four races of FOP and one nonpathogenic F. oxysporum (FO) isolate were analyzed for vegetative compatibility in order to reveal the genetic structure of the population and to check the reliability of the method for the identification of physiological races of FOP. Obtained results showed that the FOP isolates could be classified into four main vegetative compatibility groups (VCGs) that corresponded to races l, 2A, 2B and 5. The race 6 isolate fell into the race 1 VCG. To our knowledge, this is the first such study in Algeria of its kind.

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

Analysis of Vegetative Compatibility Groups of Fusarium oxysporum from Eruca vesicaria and Diplotaxis tenuifolia

2007 ◽  
Vol 155 (1) ◽  
pp. 61-64 ◽  
Author(s):  
A. Catti ◽  
M. Pasquali ◽  
D. Ghiringhelli ◽  
A. Garibaldi ◽  
M. L. Gullino
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups

Vegetative compatibility groups within Fusarium oxysporum f.sp. lini from Linum usitatissimum (flax) wilt nurseries in western Canada

2001 ◽  
Vol 79 (7) ◽  
pp. 836-843 ◽  
Author(s):  
S I Mpofu ◽  
K Y Rashid
Keyword(s):  
Population Structure ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Linum Usitatissimum ◽  
Vegetative Compatibility ◽  
Western Canada ◽  
Disease Development ◽  
Vegetative Compatibility Groups ◽  
Linum Usitatissimum L ◽  
Compatibility Analysis

Following the discovery of substantial differences in the development and severity of Fusarium wilt in Linum usitatissimum L. (flax) wilt nurseries in western Canada, a study of the population structure of Fusarium oxysporum f.sp. lini (Bolley) Snyd. & Hans using vegetative compatibility analysis was initiated. Vegetative compatibility was determined using nitrate non-utilizing mutants. From a total of 105 isolates, 74 were assigned to 12 vegetative compatibility groups (VCG 0440-04411), 22 were not compatible with any other isolates and 9 did not produce mutants. The populations of F. oxysporum f.sp. lini in Fusarium wilt nurseries in western Canada were significantly different. There was a predominant VCG in each of the nurseries, which was either nonexistent or not common in other nurseries, VCG 0440 in Indian Head, 0441 in Treherne, 0442 in Morden-80, 0443 in Saskatoon, and 0444 in Morden-60. There were only three overlapping VCGs; VCG 0440 was common to Morden-60 and Indian Head and VCGs 0442 and 0444 were common to Morden-60 and Morden-80. The differences in disease development and severity observed previously may be due to the differences in the population structure of the pathogen. These findings have significant implications for the approaches to breeding for Fusarium wilt resistance in flax.Key words: Fusarium oxysporum f.sp. lini, flax, Fusarium wilt, genetic diversity, vegetative compatibility.

Vegetative compatibility groupings in a local population of Fusarium oxysporum

1991 ◽  
Vol 69 (1) ◽  
pp. 168-172 ◽  
Author(s):  
T. R. Gordon ◽  
D. Okamoto
Keyword(s):  
Fusarium Oxysporum ◽  
Key Words ◽  
Local Population ◽  
San Joaquin Valley ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups ◽  
Heterokaryon Formation ◽  
Pathogenicity Tests

One hundred isolates of Fusarium oxysporum collected from soil in the San Joaquin Valley in 1988 were tested for their ability to form intra- and inter-isolate heterokaryons. Five thousand and fifty isolate combinations were tested for vegetative compatibility, using complementary nitrate nonutilizing mutants. Ninety-eight combinations (2%) produced weakly positive reactions, 248 (5%) produced strong reactions, and 4704 combinations (93%) were negative for heterokaryon formation. This result suggests that anastomosis occurs infrequently among isolates in this population. Isolates were assigned to vegetative compatibility groups based on strong reactions. By this procedure 100 isolates were placed in 29 vegetative compatibility groups. At least one isolate from each of the 29 vegetative compatibility groups was tested and found to be vegetatively incompatible with the muskmelon wilt pathogen, F. oxysporum f. sp. melonis, which occurs in the same field. Each of these isolates also was avirulent to muskmelon in greenhouse pathogenicity tests. Forty isolates of F. oxysporum were collected in 1989 and 32 (80%) were compatible with isolates collected in 1988. The remaining eight isolates represented eight different vegetative compatibility groups. Key words: anastomosis, fungi, heterokaryon.

scholarly journals Biological and Molecular Characterization of Fusarium oxysporum f. sp. lycopersici Divides Race 1 Isolates into Separate Virulence Groups

1999 ◽  
Vol 89 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Jurriaan J. Mes ◽  
Emma A. Weststeijn ◽  
Frits Herlaar ◽  
Joep J. M. Lambalk ◽  
Jelle Wijbrandi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Random Amplified Polymorphic Dna ◽  
Vegetative Compatibility ◽  
Avirulence Gene ◽  
Resistance Response ◽  
Vegetative Compatibility Groups ◽  
Small Set ◽  
Race 1 ◽  
Race 2 ◽  
Susceptible Tomato

A collection of race 1 and race 2 isolates of Fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic DNA (RAPD) analysis to establish the identity and genetic diversity of the isolates. Comparison of RAPD profiles revealed two main groups that coincide with vegetative compatibility groups (VCGs). In addition, several single-member VCGs were identified that could not be grouped in one of the two main RAPD clusters. This suggests that F. oxysporum f. sp. lycopersici is a polyphyletic taxon. To assign avirulence genotypes to race 1 isolates, they were tested for their virulence on a small set of tomato lines (Lycopersicon esculentum), including line OT364. This line was selected because it shows resistance to race 2 isolates but, unlike most other race 2-resistant lines, susceptibility to race 1 isolates. To exclude the influence of other components than those related to the race-specific resistance response, we tested the virulence of race 1 isolates on a susceptible tomato that has become race 2 resistant by introduction of an I-2 transgene. The results show that both line OT364 and the transgenic line were significantly affected by four race 1 isolates, but not by seven other race 1 isolates nor by any race 2 isolates. This allowed a subdivision of race 1 isolates based on the presence or absence of an avirulence gene corresponding to the I-2 resistance gene. The data presented here support a gene-for-gene relationship for the interaction between F. oxysporum f. sp. lycopersici and its host tomato.

Analysis of vegetative compatibility groups in nonpathogenic populations of Fusarium oxysporum isolated from symptomless tomato roots

1991 ◽  
Vol 69 (10) ◽  
pp. 2089-2094 ◽  
Author(s):  
Karol S. Elias ◽  
R. W. Schneider ◽  
M. M. Lear
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetable Crops ◽  
Tomato Production ◽  
Vegetative Compatibility Groups ◽  
Horticultural Traits ◽  
Tomato Roots ◽  
Constant State ◽  
Nitrate Metabolism ◽  
Tomato Cultivars

A collection of 471 isolates of Fusarium oxysporum was obtained from symptomless tomato roots of plants grown at eight locations in Louisiana. Isolates that produced nitrate metabolism mutants (317 isolates) were included in an analysis of vegetative compatibility groups. One hundred and eighty-six isolates were assigned to 48 vegetative compatibility groups (2015–2062) containing 2–15 members from one to three different collection sites. Even though each site contained sub-populations of F. oxysporum common to other sites, only about half of the vegetative compatibility groups (21 groups) contained isolates from more than one site. In addition, there were 131 isolates of F. oxysporum that formed single-member vegetative compatibility groups. This diverse population composition is probably the result of several factors, including breeding strategies for resistance to fusarium wilt and tomato production practices in Louisiana. Resistance genes and horticultural traits from several sources (Lycopersicon spp.) have been incorporated into commercial tomato cultivars currently being grown. In addition, the long growing season in Louisiana allows several tomato cultivars containing different genes for resistance to be grown consecutively in the same field in a single year. Furthermore, other vegetable crops may be alternated with the tomato crops. Thus, the selection pressures that influence the composition of the nonpathogenic populations of F. oxysporum are in a constant state of flux. Key words: Fusarium oxysporum, genetic diversity, nonpathogens, vegetative compatibility groups.

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