Dichotomic role of heparanase in a murine model of metabolic syndrome

Results of the study related to polymorphism of ACE gene (rs1799752)‎, integrin αIIbβ3, and CSK gene (rs1378942) influencing development of arterial hypertension in young patients with metabolic syndrome are presented. Hypertension as a component of the metabolic syndrome was detected in 15.0% of young patients. Prevalence of mutant alleles of the studied genes among the examined patients was quite high, so homozygous DD genotype was found in 21.6%, and mutant D allele of the ACE gene in 47.4%. A high risk of hypertension in patients with MS was detected in carriers of the T allele of the CSK (rs1378942) gene – 54.8%, which was most often observed in a combination of polymorphic ACE and CSK gene loci (p = 0.0053).

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Faculty Opinions recommendation of A diet-induced hypercholesterolemic murine model to study atherogenesis without obesity and metabolic syndrome.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1064761.517680 ◽

2007 ◽

Author(s):

Stephen Schwartz

Keyword(s):

Metabolic Syndrome ◽

Murine Model

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The Potential Role of Antioxidants in Metabolic Syndrome

Current Pharmaceutical Design ◽

10.2174/1381612822666151209152352 ◽

2016 ◽

Vol 22 (7) ◽

pp. 859-869 ◽

Cited By ~ 34

Author(s):

Bianca Martins Gregório ◽

Diogo Benchimol De Souza ◽

Fernanda Amorim de Morais Nascimento ◽

Leonardo Matta ◽

Caroline Fernandes-Santos

Keyword(s):

Metabolic Syndrome ◽

Potential Role

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Dietary Carotenoids in Managing Metabolic Syndrome and Role of PPARs in the Process

Current Nutrition & Food Science ◽

10.2174/1573401315666190619111557 ◽

2020 ◽

Vol 16 (6) ◽

pp. 846-853

Author(s):

Raghunandan Purohith ◽

Nagendra P.M. Nagalingaswamy ◽

Nanjunda S. Shivananju

Keyword(s):

Metabolic Syndrome ◽

Dietary Intervention ◽

Bioactive Constituents ◽

Dietary Antioxidants ◽

Predisposing Factor ◽

Beneficial Effects ◽

Pathological Conditions ◽

Established Relationship ◽

Comprehensive Study

Metabolic syndrome is a collective term that denotes disorder in metabolism, symptoms of which include hyperglycemia, hyperlipidemia, hypertension, and endothelial dysfunction. Diet is a major predisposing factor in the development of metabolic syndrome, and dietary intervention is necessary for both prevention and management. The bioactive constituents of food play a key role in this process. Micronutrients such as vitamins, carotenoids, amino acids, flavonoids, minerals, and aromatic pigment molecules found in fruits, vegetables, spices, and condiments are known to have beneficial effects in preventing and managing metabolic syndrome. There exists a well-established relationship between oxidative stress and major pathological conditions such as inflammation, metabolic syndrome, and cancer. Consequently, dietary antioxidants are implicated in the remediation of these complications. The mechanism of action and targets of dietary antioxidants as well as their effects on related pathways are being extensively studied and elucidated in recent times. This review attempts a comprehensive study of the role of dietary carotenoids in alleviating metabolic syndromewith an emphasis on molecular mechanism-in the light of recent advances.

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Understanding the role of oxidative stress in the incidence of metabolic syndrome and obstructive sleep apnea

BMC Endocrine Disorders ◽

10.1186/s12902-021-00735-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Behnam Kargar ◽

Zahra Zamanian ◽

Majid Bagheri Hosseinabadi ◽

Vahid Gharibi ◽

Mohammad Sanyar Moradi ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Obstructive Sleep Apnea ◽

Logistic Regression ◽

Sleep Apnea ◽

International Diabetes Federation ◽

Binary Logistic Regression ◽

Stress Index ◽

Obstructive Sleep

Abstract Background Understanding the causes and risk factors of metabolic syndrome is important for promoting population health. Oxidative stress has been associated with metabolic syndrome, and also obstructive sleep apnea. These are two diseases which have common prognostic characteristics for heart disease. The aim of this study was to examine the role of oxidative stress in the concurrent presence of metabolic syndrome and obstructive sleep apnea in a working population. Methods Participants were 163 artisan bakers in Shahroud, Iran, routinely exposed to significant heat stress and other oxidative stress indicators on a daily basis as part of their work. Using a cross-sectional design, data relevant to determining metabolic syndrome status according to International Diabetes Federation criteria, and the presence of obstructive sleep apnea according to the STOP-Bang score, was collected. Analyses included hierarchical binary logistic regression to yield predictors of the two diseases. Results Hierarchical binary logistic regression showed that oxidative stress – alongside obesity, no regular exercise, and smoking – was an independent predictor of metabolic syndrome, but not obstructive sleep apnea. Participants who were obese were 28 times more likely to have metabolic syndrome (OR 28.59, 95% CI 4.91–63.02) and 44 times more likely to have obstructive sleep apnea (OR 44.48, 95% CI 4.91–403.28). Participants meeting metabolic syndrome criteria had significantly higher levels of malondialdehyde (p < 0.05) than those who did not. No difference in oxidative stress index levels were found according to obstructive sleep apnea status. Conclusions Our findings suggest that oxidative stress contributes to the onset of metabolic syndrome, and that obstructive sleep apnea is involved in oxidative stress. Whilst obesity, exercise, and smoking remain important targets for reducing the incidence of metabolic syndrome and obstructive sleep apnea, policies to control risks of prolonged exposure to oxidative stress are also relevant in occupations where such environmental conditions exist.

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Exacerbation of AMD Phenotype in Lasered CNV Murine Model by Dysbiotic Oral Pathogens

Antioxidants ◽

10.3390/antiox10020309 ◽

2021 ◽

Vol 10 (2) ◽

pp. 309

Author(s):

Pachiappan Arjunan ◽

Radhika Swaminathan ◽

Jessie Yuan ◽

Mohamed Elashiry ◽

Amany Tawfik ◽

...

Keyword(s):

Murine Model ◽

Retinal Thickness ◽

Age Related Macular Degeneration ◽

Periodontal Pathogen ◽

Fundus Photography ◽

Mouse Retina ◽

Rrna Gene ◽

Age Related ◽

Periodontal Infection

Emerging evidence underscores an association between age-related macular degeneration (AMD) and periodontal disease (PD), yet the biological basis of this linkage and the specific role of oral dysbiosis caused by PD in AMD pathophysiology remains unclear. Furthermore, a simple reproducible model that emulates characteristics of both AMD and PD has been lacking. Hence, we established a novel AMD+PD murine model to decipher the potential role of oral infection (ligature-enhanced) with the keystone periodontal pathogen Porphyromonas gingivalis, in the progression of neovasculogenesis in a laser-induced choroidal-neovascularization (Li-CNV) mouse retina. By a combination of fundus photography, optical coherence tomography, and fluorescein angiography, we documented inflammatory drusen-like lesions, reduced retinal thickness, and increased vascular leakage in AMD+PD mice retinae. H&E further confirmed a significant reduction of retinal thickness and subretinal drusen-like deposits. Immunofluorescence microscopy revealed significant induction of choroidal/retinal vasculogenesis in AMD+PD mice. qPCR identified increased expression of oxidative-stress, angiogenesis, pro-inflammatory mediators, whereas antioxidants and anti-inflammatory genes in AMD+PD mice retinae were notably decreased. Through qPCR, we detected Pg and its fimbrial 16s-RrNA gene expression in the AMD+PD mice retinae. To sum-up, this is the first in vivo study signifying a role of periodontal infection in augmentation of AMD phenotype, with the aid of a pioneering AMD+PD murine model established in our laboratory.

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